Filamentous Fusion Phage Cloning Vectors for the Study of Epitopes and Design of Vaccines

  • Stephen F. Parmley
  • George P. Smith


Foreign sequences can be inserted within the minor coat protein, pIII, of filamentous phage, creating a fusion protein that is incorporated into the virion; the virions, which we call “fusion phage,” display the foreign amino acids encoded in the insert on their surface. Phage bearing specific antigenic determinants from a target gene can be purified in infectious form from a vast excess of phage bearing other determinants by affinity to antibody directed against the gene product. Fusion phage can be used as a source of antigen as a carrier-hapten conjugate for obtaining immunological reagents in rabbits, and for B epitope mapping. By generating a fusion phage library expressing virtually all possible short, amino acid sequences, it may be possible to study epitopes on immunologically important proteins without the use of synthetic peptides and without ever having cloned the genes.


Phage Particle Filamentous Phage Infectious Form Foreign Sequence Immunological Reagent 
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  1. Armstrong, J., Perham, R.N., and Walker, J.E. (1981), Domain structure of bacteriophage fd adsorption protein, FEBS Lett. 135, 167–172.PubMedCrossRefGoogle Scholar
  2. Crissman, J.W. and Smith, G.P. (1984), Gene-III protein of filamentous phages: evidence for a carboxyl-terminal domain with a role in morphogenesis, Virology 132, 445–455.PubMedCrossRefGoogle Scholar
  3. de la Cruz, V.F., Lal, A.A., and McCutchan, T.F. (1988), Immunogenicity and epitope mapping of foreign sequences via genetically engineered filamentous phage, J. Biol. Chem. 263, 4318–4322.PubMedGoogle Scholar
  4. Geysen, H.M., Rodda, S.M., and Mason, T.J. (1986) , A priori delineation of a peptide which mimicks a discontinuous antigenic determinant, Mol. Immunol. 23, 709–715.PubMedCrossRefGoogle Scholar
  5. Parmley, S.F. and Smith, G.P. (1988), Antibody-selectable filamentous fd phage vectors: affinity purification of target genes, Gene 73, 305–318.PubMedCrossRefGoogle Scholar
  6. Pratt, D., Tzagoloff, H., and Beaudoin, J. (1969), Conditional lethal mutants of the small filamentous coliphage M13. II. two genes for coat proteins, Virology 39, 42–53.PubMedCrossRefGoogle Scholar
  7. Smith, G.P. (1985), Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface, Science 228, 1315–1317.PubMedCrossRefGoogle Scholar
  8. Sternberg, N. and Hamilton, D. (1981), Bacteriophage P1 site-specific recombination. I. recombination between loxP sites, J. Mol. Biol. 150, 467–486.PubMedCrossRefGoogle Scholar
  9. Sternberg, N., Sauer, B., Hoess, R., and Abremski, K. (1986), Bacteriophage P1 cre gene and its regulatory region, J. Mol. Biol. 187, 197–212.PubMedCrossRefGoogle Scholar
  10. Van Cleave, V. H., Naeve, C. W., and Metzger, D.W. (1988), Do antibodies recognize amino acid side chains of protein antigens independently of the carbon backbone?, J. Exp. Med. 167, 1841–1848.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Stephen F. Parmley
    • 1
  • George P. Smith
    • 2
  1. 1.Department of Immunology and Infectious Diseases Research InstitutePalo Alto Medical FoundationPalo AltoUSA
  2. 2.Division of Biological Sciences, Tucker HallUniversity of Missouri ColumbiaUSA

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