Vaccine-Containing Biodegradable Microspheres Specifically Enter the Gut-Associated Lymphoid Tissue Following Oral Administration and Induce a Disseminated Mucosal Immune Response

  • John H. Eldridge
  • Jonathan A. Meulbroek
  • Jay K. Staas
  • Thomas R. Tice
  • Richard M. Gilley

Abstract

Biodegradable and biocompatible microspheres have been investigated for their usefulness as a vaccine delivery system for both parenteral and enteral immunization. Microspheres composed of poly (DL-lactide-co-glycolide) which contained a toxoid vaccine of Staphylococcal enterotoxin B were found to strongly potentiate the circulating anti-toxin antibody response following intraperitoneal injection. Following oral administration, microspheres < 10 µm in diameter were specifically taken up into the Peyer’ s patches of the gutassociated lymphoid tissue, where those > 5 µm remained fixed for an extended period. Microspheres < 5 µm were disseminated within macrophages to the mesenteric lymph nodes, blood circulation and spleen. Oral immunization with enterotoxoidcontaining microspheres induced circulating toxin-specific antibodies and a concurrent secretory IgA anti-toxin response in saliva, gut wash fluids and bronchial-alveolar wash (BAW) fluids. In contrast, soluble enterotoxoid was completely ineffective as an oral immunogen.

Keywords

Mesenteric Lymph Node Staphylococcal Enterotoxin Secretory Component Oral Immunization Biodegradable Microsphere 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • John H. Eldridge
    • 1
  • Jonathan A. Meulbroek
    • 1
  • Jay K. Staas
    • 2
  • Thomas R. Tice
    • 2
  • Richard M. Gilley
    • 2
  1. 1.Department of MicrobiologyUniversity of Alabama at BirminghamBirminghamUSA
  2. 2.Controlled Release DivisionSouthern Research InstituteBirminghamUSA

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