The Use of Mouse Class II MHC Peptides to Produce Site-Specific Monoclonal Antibodies

Abstract

We have attempted to produce site-specific monoclonal antibodies against defined regions on mouse class II (Ia) molecules. These studies were designed to produce monoclonal antibodies against defined sequences of Ia molecules which may have applications in the design of novel experimental , functional and therapeutic investigations into the biochemical role of Ia molecules in immunity and disease states (Rothbard and Taylor , 1988) . We describe here our initial results using peptides derived from the polymorphic regions 43–61 of the alpha chain subunit and polymorphic regions 57–74/78 of beta chain subunit of I-A molecules from three alleles .