The Use of Mouse Class II MHC Peptides to Produce Site-Specific Monoclonal Antibodies

  • Christopher J. Krco
  • Thomas G. Beito
  • Roger G. Little
  • Jay Zeller
  • Daniel M. McCormick
  • Chella S. David

Abstract

We have attempted to produce site-specific monoclonal antibodies against defined regions on mouse class II (Ia) molecules. These studies were designed to produce monoclonal antibodies against defined sequences of Ia molecules which may have applications in the design of novel experimental , functional and therapeutic investigations into the biochemical role of Ia molecules in immunity and disease states (Rothbard and Taylor , 1988) . We describe here our initial results using peptides derived from the polymorphic regions 43–61 of the alpha chain subunit and polymorphic regions 57–74/78 of beta chain subunit of I-A molecules from three alleles .

Keywords

Mouse Class Individual Seron Subunit Peptide Automate Peptide Synthesizer Mayo Medical School 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Krco, C.J. , Gorzynski, T.J. and Beito, T. (1985) Characterization of a monoclonal antibody that recognizes a determinant unique to human haemoglobin β-chain. J. Immunogenetics 12:197–211.CrossRefGoogle Scholar
  2. McCormick, D.J . and Atassi, M.Z. (1985) Antigenic structure of human hemoglobin: Delineation of the antigenic sites (Site 2) within region 4165 of the alpha chain by immunochemistry of synthetic peptides. J . Protein Chemistry 4: 171–184.CrossRefGoogle Scholar
  3. Rothbard, J .B. and Taylor, W.R. (1988) A sequence pattern common to the T cell epitopes. EMBO Journal 7: 93–100.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Christopher J. Krco
    • 1
  • Thomas G. Beito
    • 1
  • Roger G. Little
    • 1
  • Jay Zeller
    • 1
  • Daniel M. McCormick
    • 1
  • Chella S. David
    • 1
  1. 1.Departments of Immunology and BiochemistryMayo Medical School, Mayo ClinicRochesterUSA

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