Application of 1H NMR Urinalysis to the Examination of Nephrotoxic Lesions Induced by Mercuric Chloride, Hexachloro-1, 3-Butadiene, and Propyleneimine
In recent years proton nuclear magnetic resonance (1H NMR) spectroscopic analysis of urine has been shown to provide sensitive indications of renal damage induced in rats by mercuric chloride (1) and p-aminophenol (2,3). Notable among these is the excretion of considerable quantities of glucose and lactic acid following p-aminophenol (2,3). Both of these parameters were later quantified employing conventional UV methods and found to be dose-related. Mercuric chloride has also been reported to produce a considerable lacticaciduria in fasted Sprague-Dawley rats (1). Prior to these studies, the appearance of lacticaciduria following nephrotoxic insult had not been reported.
KeywordsCorn Depression Urea Lactate Citrate
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- 1.J.K. Nicholson, J.A. Timbrell and P.J. Sadler, Proton NMR Spectra of urine as indicators of renal damage. Mercury-induced nephrotoxicity in rats, Mol. Pharamacol., 27:644–651 (1985).Google Scholar
- 2.K.P.R. Gartland, J.K. Nicholson, J.A. Timbrell and F.W. Bonner, Detection and assessment of p-aminophenol-induced nephrotoxic lesions by Proton NMR spectroscopy, Human Tox. 5:122–123 (1986).Google Scholar