Nephrotoxicity pp 439-445 | Cite as

Celiptium Induced Lipid Peroxidation and Toxicity in Rat Renal Cortex

  • G. Raguenez-Viotte
  • C. Dadoun
  • A. M. Van den Bossche
  • J. P. Fillastre

Abstract

The antitumour drug Celiptium (N2-methyl-9-hydroxyellipticinium) is an ellipticine derivative, effective in experimental tumours (1) and in man. Celiptium is metabolized in liver and excreted by the biliary tract (60%) and urine (30%). However, a renal metabolism occurs in both rats and humans treated with Celiptium, cysteine and N-acetylcysteine conjugates appear in the urine (2). Moreover, it was shown that isolated rat kidney cells metabolize Celiptium into the same conjugates which were found in rat or human urines whereas these compounds were not detected in bile (3).

Keywords

Fatty Acid Composition Fatty Acid Methyl Ester Neutral Lipid Renal Cortex Total Phospholipid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    C. Paoletti, J.B. Le Pecq, N. Dat Xuong, P. Juret, H. Garnier, J.L. Amiel, J. Rouesse, Antitumor activity, pharmacology and toxicity of ellipticines, ellipticinium and 9-hydroxy-derivatives: preliminary clinical trials of 2-methyl-9-hydroxy-ellipticinium (NSC-264–137). Recent Results Canc. Res., 74:107 (1980)CrossRefGoogle Scholar
  2. 2.
    B. Monsarrat, M. Maftouh, G. Meunier, B. Dugue, J. Bernadou, J.P. Armand, C. Picard-Fraire, B. Meunier, C. Paoletti, Human and rat urinary metabolites of the hydroxy methylellipticinium. Identification of cysteine conjugates supporting the bioxidative alkylation hypothesis. Biochem. Pharmacol., 32:3887 (1983)PubMedCrossRefGoogle Scholar
  3. 3.
    M. Maftouh, Y. Amiar, C. Picard-Fraire. Metabolism of the antitumor drug N2-methyl-9-hydroxyellipticinium acetate in isolated rat kidney cells. Biochem. Pharmacol., 34:427 (1985)CrossRefGoogle Scholar
  4. 4.
    P. Juret, A. Tanguy, A. Girard. L’ acitate d’ hydroxy-9-mithyl-2-ellipticinium. Etude toxicologique et thirapeutique chaz 100 cancereux. Nouv. Presse Med. 8:1494 (1978)Google Scholar
  5. 5.
    G. Raguinez-Viotte, C. Dadoun, P. Buchet, T. Ducastelle, J.P. Fillastre. Renal toxicity of the antitumor drug N2-methyl-9-hydroxy-ellipticinium acetate in the Wistar rat. Submitted Arch Toxicology. Google Scholar
  6. 6.
    O.B. Bayliss-High, Lipids In: Theory and practice of histological techniques, Bancroft, edited by J. D. Stevens, Melbourne-New York. p.217 (1982)Google Scholar
  7. 7.
    O. H. Lowry, N.J. Rosebrough, A.L. Farr, R.J. Randall, Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265 (1951)PubMedGoogle Scholar
  8. 8.
    E.G. Bligh and W.J. Dyer. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37:911 (1959)PubMedCrossRefGoogle Scholar
  9. 9.
    J.A. Buege and S.D. Aust, Microsomal lipid peroxidation. Methods Enzymol. 52:302 (1978)PubMedCrossRefGoogle Scholar
  10. 10.
    H. Ohkawa, N. Ohishi, K. Yagi, Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95:351 (1979)PubMedCrossRefGoogle Scholar
  11. 11.
    V.P. Skipski, R.F. Peterson, M. Barclay, Quantitative analysis of phospholipids by thin layer chromatography. Biochem. J. 90:374 (1964)PubMedGoogle Scholar
  12. 12.
    G.R. Bartlett, Phosphorus assay in column chromatography. J. Biol. Chem. 234:466 (1959)PubMedGoogle Scholar
  13. 13.
    W.R. Morrison and L.M. Smith, Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron trifluoride-methanol. J. Lipid Res. 5:600 (1964)PubMedGoogle Scholar
  14. 14.
    A.L. Tappel, Lipid peroxidation damage to cell components. Fed. Proc. 32:1870 (1973)PubMedGoogle Scholar
  15. 15.
    H. Kappus, Lipid peroxidation: mechanisms, analysis, enzymology and biological relevance. In: “Oxidative stress” edited by H. Sies, Academic Press. p. 273 (1985)Google Scholar
  16. 16.
    C. Auclair, K. Hyland and C. Paoletti. Autooxidation of the antitumor drug 9-hydroxyellipticine and its derivatives. J. Med. Chem. 26:1438 (1983)PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • G. Raguenez-Viotte
    • 1
  • C. Dadoun
    • 1
  • A. M. Van den Bossche
    • 1
  • J. P. Fillastre
    • 1
  1. 1.INSERM U-295Universite de RouenSaint Etienne du RouvrayFrance

Personalised recommendations