Red Blood Cell Negative Charges as an Index of the Glomerular Polyanion in Chronic Cadmium Poisoning

  • Alfred Bernard
  • Ali Ouled Amor
  • Harry Roels
  • Robert Lauwerys

Abstract

Fixed negative charges on the glomerular capillary wall (glomerular polyanion) provide an electrostatic barrier to the filtration of anionic macromolecules such as albumin (1). Loss of glomerular polyanion in clinical or experimental renal diseases is associated with an enhanced excretion of anionic molecules. Evidence has been presented by recent studies (2, 3) that the negative charges of the red blood cells (RBCs) membrane, as determined by the binding of the cationic dye Alcian blue (AB) might reflect the charges of the glomerular polyanion. Applying this AB binding test to RBCs from cadmium (Cd)-exposed workers and to both RBCs and isolated glomeruli from Cd-treated rats, we demonstrate here that, at an early stage of the intoxication, Cd can decrease negative charges of RBCs membrane and that this phenomenon reflects a concomitant loss of the charge selectivity of the glomerular basement membrane.

Keywords

Alcian Blue Glomerular Basement Membrane Alcian Blue Glomerular Capillary Wall Charge Selectivity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Alfred Bernard
    • 1
  • Ali Ouled Amor
    • 1
  • Harry Roels
    • 1
  • Robert Lauwerys
    • 1
  1. 1.Unite de Toxicologie Industrielle et Medecine du TravailUniversite Catholique de LouvainBrusselsBelgium

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