Red Blood Cell Negative Charges as an Index of the Glomerular Polyanion in Chronic Cadmium Poisoning
Fixed negative charges on the glomerular capillary wall (glomerular polyanion) provide an electrostatic barrier to the filtration of anionic macromolecules such as albumin (1). Loss of glomerular polyanion in clinical or experimental renal diseases is associated with an enhanced excretion of anionic molecules. Evidence has been presented by recent studies (2, 3) that the negative charges of the red blood cells (RBCs) membrane, as determined by the binding of the cationic dye Alcian blue (AB) might reflect the charges of the glomerular polyanion. Applying this AB binding test to RBCs from cadmium (Cd)-exposed workers and to both RBCs and isolated glomeruli from Cd-treated rats, we demonstrate here that, at an early stage of the intoxication, Cd can decrease negative charges of RBCs membrane and that this phenomenon reflects a concomitant loss of the charge selectivity of the glomerular basement membrane.
KeywordsAlcian Blue Glomerular Basement Membrane Alcian Blue Glomerular Capillary Wall Charge Selectivity
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