Effect of Latamoxef [Moxalactam] on Tobramycin Binding to Kidney Brush Border Membranes in Rats.
Latamoxef (LMOX) [moxalactam] has previously been shown to protect rat kidneys from tobramycin (TOB) -induced nephrotoxicity (1,2), and the mechanism for the protection may be involved in the suppression of intrarenal TOB accumulation by combination with LMOX. Therefore, to clarify the mechanism by which LMOX suppresses the intrarenal TOB accumulation in vivo, we have hypothesised that positively charged TOB may be able to interact with negatively charged LMOX by ionic binding in vivo and the interaction may inhibit the binding of TOB to acidic phospholipids forming brush border membranes (BBMs). The inhibition of TOB binding to BBMs may contribute to the decrease in the intrarenal TOB accumulation. Recently, we have demonstrated that TOB directly interacts with LMOX in vitro, (3) and the interaction is associated with the suppression of intrarenal TOB accumulation by combination with LMOX in vivo.
KeywordsBasolateral Membrane Brush Border Membrane Ammonium Molybdate Aminoglycoside Antibiotic Adenosine Triphosphatase
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