Nephrotoxicity pp 659-662 | Cite as

The Isolated Perfused Rat Kidney: Filtering and Non-Filtering Models in the Assessment of Altered Renal Vascular Resistance in Nephrotoxicity

  • Peter Ratcliffe
  • Zoltan Endre
  • Lynn Nicholls
  • John Tange
  • John Ledingham

Abstract

A reduction in renal blood flow arising from increased vascular resistance is observed in most models of nephrotoxic acute renal failure, and may exacerbate renal damage by compounding ischaemic with non-ischaemic injury (1). These changes in vascular resistance may arise from a direct vascular effect of the toxin or be secondary to tubular injury. We have used the isolated perfused rat kidney to address this issue in a model of mercuric chloride-induced acute renal failure. In the filtering isolated perfused kidney, a controlled exposure to a nephrotoxin can be made in a manner comparable to in vivo. To create a non-filtering preparation, the oncotic pressure of the perfusate was raised to stop glomerular filtration and hence luminal exposure to the nephrotoxin, whilst vascular and peritubular exposure were unchanged.

Keywords

Mercuric Chloride Urine Flow Rate Renal Vascular Resistance Oncotic Pressure Inulin Clearance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    T. H. Hostetter, B.M. Wilkes, and B.M. Brenner, Renal circulatory and nephron function in experimental acute renal failure, Chapter 4, In: “Acute Renal Failure”, B.M. Brenner and J.M. Lazarus, ed., Publisher, W.R. Saunders (1983).Google Scholar
  2. 2.
    F.H. Epstein, J.T. Brosnan, J.D. Tange, and B.D. Ross, Improved function with amino acids in the isolated perfused kidney, Am. J. Physiol. 243:E284–F292 (1982).Google Scholar
  3. 3.
    Z.H. Endre, L.G. Nicholls, P.J. Ratcliffe, and J.G.G. Ledingham, Prevention and reversal of mercuric chloride-induced increases in renal vascular resistance by Captopril (this Symposium).Google Scholar

Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Peter Ratcliffe
    • 1
  • Zoltan Endre
    • 1
  • Lynn Nicholls
    • 1
  • John Tange
    • 1
  • John Ledingham
    • 1
  1. 1.Nuffield Department of Clinical MedicineJohn Radcliffe HospitalOxfordUK

Personalised recommendations