The GTP-Binding Domain Revisited

  • Thomas E. Dever
  • William C. Merrick


Recently we explored the similarity of several GTP-binding domains using principals developed from newly reported structural features of EF-Tu (1–4). In this study, it was concluded that many GTP-binding proteins contain the following consensus sequence elements (GXXXXGK, DXXG, and NKXD) and that these elements were spaced in one of two patterns: the distance between the first and second consensus elements was either 40 to 80 amino acids or about 150 amino acids while the second and third consensus elements were usually 40 to 80 amino acids apart. An updated presentation of GTP-binding proteins which conform to this general scheme is given in Tables 1, 2, and 3 although additional sequences of the three major families have not been included (EF-Tu and EF-1a; RAS, YP2, and rho; G proteins). Each of these individual families will be the subject of more extensive study in other chapters in this book. In addition, there will be no discussion of a or ß tubulin, both GTP-binding proteins, as neither of these proteins contains the three consensus elements.


Consensus Element Consensus Sequence Element 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Dever, T.E., Glynias, M.J., and Merrick, W.C. (1987) Proc. Natl. Acad. Sci. USA 84, 1814–1818.PubMedCrossRefGoogle Scholar
  2. 2.
    la Cour, T.F.M., Nyborg, J., Thirup, S. and Clark, B.F.C. (1985) EMBO J. 4, 2385–2388.PubMedGoogle Scholar
  3. 3.
    McCormick, F., Clark, B.F.C., la Cour, T.F.M., Kjeldgaard, M., Norskov-Lauritsen, L. and Nyborg, J. (1985) Science 230, 78–82.PubMedCrossRefGoogle Scholar
  4. 4.
    Jurnak, F. (1985) Science 230, 32–36.PubMedCrossRefGoogle Scholar
  5. 5.
    Arai, K., Clark, B.F.C., Duffy, L., Jones, M.D., Kaziro, Y., Laursen, R.A., L’Italien, J., Miller, D.L., Nagarkatti, S., Nakamura, S., Nielsen, K.M., Petersen, T.E., Takahashi, K. and Wade, M. (1980) Proc. Natl. Acad. Sci. USA 77, 1326–1330.PubMedCrossRefGoogle Scholar
  6. 6.
    Montandon, P. and Stutz, E. (1983) Nucleic Acids Res. 11, 5877–5892.PubMedCrossRefGoogle Scholar
  7. 7.
    Nagata, S., Tsunetsugu-Yokota, Y., Naito, A. and Kaziro, Y. (1983) Proc. Natl. Acad. Sci. USA 80, 6192–6196.PubMedCrossRefGoogle Scholar
  8. 8.
    Nagata, S., Nagashima, K., Tsunetsugu-Yokota, Y., Fujimura, K., Miyazaki, M. and Kaziro, Y. (1984) EMBO J. 3, 1825–1830.PubMedGoogle Scholar
  9. 9.
    Cottrelle, P., Thiele, D., Price, V.L., Memet, S., Micouin, J.Y., Marck, C, Buhler, J.M., Sentenac, A. and Fromagoet, P. (1985) J. Biol. Chem. 260, 3090–3096.PubMedGoogle Scholar
  10. 10.
    Van Hemert, F.J., Amons, R., Pluijms, W.J.M., Van Ormondt, H. and Moller, W. (1984) EMBO J. 3, 1109–1113.PubMedGoogle Scholar
  11. 11.
    Brands, J.H.G.M., Maassen, J.A., Van Hemert, F.J., Amons, R. and Moller, W. (1986) Eur. J. Biochem. 155, 167–171.PubMedCrossRefGoogle Scholar
  12. 12.
    Ovchinnikov, Y.A., Alakhov, Y.B., Bundulis, Y.P., Bundule, M.A., Dovgas, N.V., Kozlov, V.P., Motuz, L.P. and Vinokurov, L.M. (1982) FEBS Lett. 139, 130–135.PubMedCrossRefGoogle Scholar
  13. 13.
    Kohno, K., Uchida, T., Ohkubo, H., Nakanishi, S., Nakanishi, T., Fukui, T., Ohtsuka, E., Ikehara, M. and Okada, Y. (1986) Proc. Natl. Acad. Sci. USA 83, 4978–4982.PubMedCrossRefGoogle Scholar
  14. 14.
    March P.E. and Inouye, M. (1985) Proc. Natl. Acad. Sci. USA 82, 7500–7504.PubMedCrossRefGoogle Scholar
  15. 15.
    Sacerdot, C., Dessen, P., Hershey, J.W.B., Plumbridge, J.A. and Grunberg-Manago, M. (1984) Proc. Natl. Acad. Sci. USA 81, 7787–7791.PubMedCrossRefGoogle Scholar
  16. 16.
    DeFoe-Jones, D., Scolnick, E.M., Koller, R. and Dhar, R. (1983) Nature 306, 707–709.CrossRefGoogle Scholar
  17. 17.
    Powers, S., Kataoka, T., Fasano, O., Goldfarb, M., Strathern, J., Broach, J. and Wigier, M. (1984) Cell 36, 607–612.PubMedCrossRefGoogle Scholar
  18. 18.
    Dahr, R., Nieto, A., Koller, R., DeFoe-Jones, D. and Scolnick, E. M. (1984) Nucleic Acids Res. 12, 3611–3618.CrossRefGoogle Scholar
  19. 19.
    Gallwitz, D., Donath, C. and Sander, C. (1983) Nature 30, 704–707.CrossRefGoogle Scholar
  20. 20.
    Capon, D.J., Chen, E.Y., Levinson, A.D., Seeburg, P.H. and Goeddel, D.V. (1983) Nature 302, 33–37.PubMedCrossRefGoogle Scholar
  21. 21.
    Taparowsky, E., Shimizu, K., Goldfarb, M. and Wigier, M. (1983) Cell 34, 581–586.PubMedCrossRefGoogle Scholar
  22. 22.
    Shimizu, K., Birnbaum, D., Ruley, M., Fasano, O., Suard, Y., Edlund, L., Taparowsky, E., Goldfarb, M. and Wigier, M. (1983) Nature 304, 497–500.PubMedCrossRefGoogle Scholar
  23. 23.
    Rasheed, S., Norman, G.L. and Heidecker, G. (1983) Science 221, 155–157.PubMedCrossRefGoogle Scholar
  24. 24.
    Reddy, E.P., Lipman, D., Andersen, P.R., Tronick, S.R. and Aaronson, S.A. (1985) J. Virol. 53, 984–987.PubMedGoogle Scholar
  25. 25.
    Dhar, R., Ellis, R.W., Shih, T.Y., Oroszlan, S., Shapiro, B., Maizel, J., Lowy, D. and Scolnick, E. (1982) Science 217, 934–937.PubMedCrossRefGoogle Scholar
  26. 26.
    Tsuchida, N., Ryder, T. and Ohtsubo, E. (1982) Science 217, 937–939.PubMedCrossRefGoogle Scholar
  27. 27.
    Madaule, P. and Axel, R. (1985) Cell 41, 31–40.PubMedCrossRefGoogle Scholar
  28. 28.
    Cook, J.S., Weldon, S.L., Garcia-Ruiz, J.P., Hod Y. and Hanson, R.W. (1986) Proc. Natl. Acad. Sci. USA 83, 7583–7587.PubMedCrossRefGoogle Scholar
  29. 29.
    Weldon, S. L. & Hanson, R.W., personal communication.Google Scholar
  30. 30.
    Beale, L.G., Chrapkiewicz, N.B., Scoble, H.A., Metz, R.J., Quick, D.G., Noble, R.L, Donelson, J.E., Biemann, K. and Granner, D.K. (1985) J. Biol. Chem. 260, 10748–10760.PubMedGoogle Scholar
  31. 31.
    Gundelfinger, E.D., Hermans-Borgmeyer, I., Grenningloh, G. and Zopf, D. (1987) Nuc. Acids Res. 15, 6745.CrossRefGoogle Scholar
  32. 32.
    Tomasselli, A.G., Frank, R. and Schiltz, E. (1986) FEBS Lett. 202, 303–308.PubMedCrossRefGoogle Scholar
  33. 33.
    Seliger, L.S., Zheng, K. and Shatkin, A.J. (1987) J. Biol. Chem. 262, 16289–16293.PubMedGoogle Scholar
  34. 34.
    Yatsunami, K. and Khorana, H.G. (1985) Proc. Natl. Acad. Sci. USA 82, 4316–4320.PubMedCrossRefGoogle Scholar
  35. 35.
    Tanabe, T., Nukada, T., Nishikawa, Y., Sugimoto, K., Suzuki, H., Takahashi, H., Noda, M., Haga, T., Ichiyama, A., Kangawa, K., Minammo, N., Matsuo, H. and Numa, S. (1985) Nature 315, 242–245.PubMedCrossRefGoogle Scholar
  36. 36.
    Robishaw, J.D., Russell, D.W., Harris, B.A., Smigel, M.D. and Gilman, A.G. (1986) Proc. Natl. Acad. Sci. USA 83, 1251–1255.PubMedCrossRefGoogle Scholar
  37. 37.
    Itoh, H., Kozasa, T., Nagata, S., Nakamura, S., Katada, T., Ui, M., Iwai, S., Ohtsuka, E., Kawasaki, H., Suzuki, K. and Kaziro, Y. (1986) Proc. Natl. Acad. Sci. USA 83, 3776–3780.PubMedCrossRefGoogle Scholar
  38. 38.
    Fry, D.C., Kuby, S.A. and Mildvan, A.S. (1986) Proc. Natl. Acad. Sci. U.S.A. 83, 907–911.PubMedCrossRefGoogle Scholar
  39. 39.
    Moller, W. and Amons, R. (1985) FEBS Lett. 186, 1–7.PubMedCrossRefGoogle Scholar
  40. 40.
    Carroll, A.R., Rowlands, D.J. and Clarke, B.E. (1984) Nucleic Acids Res. 12, 2461–2472.PubMedCrossRefGoogle Scholar
  41. 41.
    Kikuchi, Y., Shimatake, H. and Kikuchi, A. (1988) EMBO J. 7, 1175–1182.PubMedGoogle Scholar
  42. 42.
    Sanchez-Pescador, R., Brown, J.T., Roberts, M. and Urdea, M.S. (1988) Nuc. Acids Res. 16, 1216–1217.CrossRefGoogle Scholar
  43. 43.
    Sanchez-Pescador, R., Brown, J.T., Roberts, M. and Urdea, M.S. (1988) Nuc. Acids Res. 16, 1218.CrossRefGoogle Scholar
  44. 44.
    Hwang, Y. and Miller, D.L (1987) J. Biol. Chem. 262, 13081–13085.PubMedGoogle Scholar
  45. 45.
    T. Nowak, personal communication.Google Scholar
  46. 46.
    A. Shatkin, personal communication.Google Scholar
  47. 47.
    Heil, A., Muller, G., Noda, L.H., Pinder, T., Schirmer, R.H., Schirmer, I. and Von Zabern, I. (1974) Eur. J. Biochem. 43, 131–144.PubMedCrossRefGoogle Scholar
  48. 48.
    Pai, E.F., Sachsenheimer, W., Schirmer, R.H., and Schulz, G.E. (1977) J. Mol. Biol. 114, 37–45.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Thomas E. Dever
    • 1
  • William C. Merrick
    • 1
  1. 1.Department of Biochemistry, School of MedicineCase Western Reserve UniversityClevelandUSA

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