Tubulin Structure and Nucleotide Binding

  • Eva-Maria Mandelkow
  • Klaus Linse
  • Eckhard Mandelkow


In this report we summarize our recent studies to identify the GTP binding site of tubulin, the subunit of microtubules, using the method of direct photoaffinity labeling. We compare the results with predictions derived from a comparison of tubulin with other nucleotide binding proteins. The binding site of the base (peptide 63–77) is consistent with that of other GTP binding proteins; however, its position relative to other presumptive sites (e.g. the phosphate binding loop) differs from the consensus pattern of G-proteins. This means that tubulin represents a distinct class of nucleotide binding proteins.


Tryptic Peptide Consensus Pattern Base Binding Presumptive Site Subtilisin Type 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Eva-Maria Mandelkow
    • 1
  • Klaus Linse
    • 1
  • Eckhard Mandelkow
    • 1
  1. 1.Max-Planck-Unit for Structural Molecular BiologyHamburg 52Germany

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