Tubulin Structure and Nucleotide Binding
In this report we summarize our recent studies to identify the GTP binding site of tubulin, the subunit of microtubules, using the method of direct photoaffinity labeling. We compare the results with predictions derived from a comparison of tubulin with other nucleotide binding proteins. The binding site of the base (peptide 63–77) is consistent with that of other GTP binding proteins; however, its position relative to other presumptive sites (e.g. the phosphate binding loop) differs from the consensus pattern of G-proteins. This means that tubulin represents a distinct class of nucleotide binding proteins.
KeywordsTryptic Peptide Consensus Pattern Base Binding Presumptive Site Subtilisin Type
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