Abstract
Most of the studies on signaltransduction and the role of G-proteins therein have been done on mammalian cells. This brings about several disadvantages, such as laborious growth conditions non-homogeneous material and the use of highly specialized cells. The use of tumor cell lines brings the risk of using cells in which especially the signaltransduction pathways are somehow impaired. Using micro-organisms can overcome many of these disadvantages. In our studies we use the cellular slime mold Dictyostelium discoideum which is an easy to grow haploid organism with a simple life cycle. The fact that it is haploid makes it easier to perform both classical as well as molecular genetics. Dictyostelium discoideum is a soil living organism which feeds on bacteria during its vegetative stage. Upon food deprivation it enters a social stage in which cells aggregate into multicellular structures in response to excreted cAMP. Subsequently differentiation to two different cell types (i.e. spores and stalk cells) takes place (Schaap 1986) . In the aggregative stage cAMP elicits several cellular responses which are thought to be mediated by two types of cAMP surface receptors, the A sites which exist in a low (AL) and a high (AH) affinity form and the B sites of which three kinetic forms can be distinguished. A fast dissociating form (BF) a slower dissociating form (Bs) and a very slowly dissociating form (Bss) (Van Haastert, et al. 1986) .
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Literature Cited
Coukell, M. B., S. Lappano, and A. M. Cameron. 1983. Isolation and characterization of cAMP unresponsive (frigid) aggregation deficient mutants of Dictyostelium discoideum. Dev.Genet. 3:283–297
Devreotes, P. N. 1983. Cyclic nucleotides and cell-cell communication in Dictyostelium discoideum. Adv.Cyclic Nucleotide Res. 15:55–96.
Europe-Finner, G. N. and P. C. Newell. 1987. GTP-analogues stimulate inositol trisphosphate formation transiently in Dictyostelium. J.Cell Sci. 87:513–518.
Franz, C. E. 1980. Phenotype analysis of aggregation mutants of Dictyostelium discoideum. PhD Thesis, University of Chicago, Chicago.
Gilman, A. G. 1987. G-proteins: transducers of receptor generated signals. Ann.Rev.Biochem. 56:615–649.
Janssens, P. M. W. and P. J. M. Van Haastert. 1987. Molecular basis of transmembrane signal transduction in Dictyostelium discoideum. Microbiol.Rev. 51:396–418.
Kesbeke, F., B. E. Snaar-Jagalska, and P. J. M. Van Haastert. 1988. Signal-transduction in Dictyostelium fgd A mutants with a defective interaction between surface cAMP receptor and a GTP binding regulatory protein. J.Cell.Biol. 107:521–528
Leichtling, B. H., D. S. Coffman, E. S. Yaeger, H. V. Rickenberg, W. al-Jumaliy, and B. E. Haley. 1981. Occurence of the adenylate cyclase “G-protein” in membranes of Dictyostelium discoideum. Biochem.Biophys.Res.Comm. 102:1187–1195.
Levitzki, A. 1988. Signal transduction in hormone dependent adenylate cyclase. Cell.Biophys. 12:133–143.
Mattera, R., J. Codina, R. D. Sekura, and L. Birnbaumer. 1986. The interaction of nucleotides with pertussis toxin. J.Biol.Chem. 261:11173–11179.
Mumby, S. M., R. A. Kahn, D. R. Manning, and A. G. Gilman. 1986. Antisera of designed specificity for subunits of guanine nucleotide binding regulatory proteins. Proc.Natl.Acad.Sci.USA 83:265–269.
Schaap, P. 1986. Regulation of size and pattern in the cellular slime molds. Diff. 33:1–16.
Snaar-Jagalska, B. E. and P. J. M. Van Haastert. 1988. Pertussis toxin inhibits cAMP-induced desensitization of adenylate cyclase in Dictyostelium discoideum submitted.
Snaar-Jagalska, B. E. and P. J. M. Van Haastert. 1988. Dictyostelium discoideum mutant synag 7 with altered adenylate cyclase interaction. J,Cell.Sci. in press.
Snaar-Jagalska, B. E., R. J. W. De Wit, and P. J. M. Van Haastert. 1988a. Pertussis toxin inhibits cAMP surface receptor stimulated binding of 35S GTPγS to Dictyostelium discoideum membranes. FEBS Lett. 232:148–152.
Snaar-Jagalska, B. E., K. H. Jakobs, and P. J. M. Van Haastert. 1988b. Agonist stimulated high affinity GTP-ase in Dictyostelium discoideum. FEBS Lett. 236:139–144.
Snaar-Jagalska, B. E., F. Kesbeke, M. Pupillo, and P. J. M. Van Haastert. 1988c. Immunological detection of G-protein alpha subunits in Dictyostelium discoideum. Biochim.Biophys.Rec.Comm. in press.
Van Haastert, P. J. M., R. J. W. De Wit, P. M. W. Janssens, F. Kesbeke, and J. DeGoede. 1986. G-protein mediated interconversions of cell surface cAMP receptors and their involvement in excitation and desensitization of guanylate cyclase in Dictyostelium discoideum. J.Biol.Chem. 261:6904–6911.
Van Haastert, P. J. M., L. C. Penning, and F. Kesbeke. 1988. Dictyostelium mutants with malfunctioning G-proteins reveal cross talk of signal transduction pathways, submitted.
Van Haastert, P. J. M., B. E. Snaar-jagalska, and P. M. W. Janssens. 1987. The regulation of adenylate cyclase by guanine nucleotides in Dictyostelium discoideum membranes. Eur.J.Biochem. 162:251–258
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Springer Science+Business Media New York
About this chapter
Cite this chapter
Bominaar, A.A., Snaar-Jagalska, B.E., Kesbeke, F., Haastert, P.J.M.V. (1989). Signal-Transducing G-Proteins in Dictyostelium Discoideum . In: Bosch, L., Kraal, B., Parmeggiani, A. (eds) The Guanine — Nucleotide Binding Proteins. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-2037-2_36
Download citation
DOI: https://doi.org/10.1007/978-1-4757-2037-2_36
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-2039-6
Online ISBN: 978-1-4757-2037-2
eBook Packages: Springer Book Archive