Structure and Function of G Proteins from Mammalian and Yeast Cells

  • Yoshito Kaziro


A superfamily of GTP binding proteins consists of several families including (1) translational factors, (2) signal transducing G proteins, (3) protooncogenic ras proteins, and (4) other low-molecular-weight GTP binding proteins such as rho, sec (sec4, YPTl, etc.) and arf families. There are also other GTP-binding proteins such as tubulins and a group of metabolic GTP-binding proteins including succinate thiokinase and phosphoenolpyruvate carboxykinase, but they will not be discussed here.


Adenylate Cyclase Cholera Toxin Pertussis Toxin Polypeptide Chain Elongation Succinate Thiokinase 
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  1. Barbacid, M., 1987, ras genes. Annu. Rev. Biochem., 56:779–827.PubMedCrossRefGoogle Scholar
  2. Bray, P., Carter, A., Simons, C., Guo, V., Puckett, C., Kamholz, J., Spiegel, A. , and Nirenberg, M., 1986, Human cDNA clones for four species of Gas signal transduction protein. Proc. Natl. Acad. Sci. USA, 83:8893–8897.PubMedCrossRefGoogle Scholar
  3. Broek, D., Samiy, N., Fasano, O., Fujiyama, A., Tamanoi, F., Northup, J., and Wigler, M., 1985, Differential activation of yeast adenylate cyclase by wild-type and mutant RAS proteins. Cell, 41:763–769.PubMedCrossRefGoogle Scholar
  4. DeFeo-Jones, D., Scolnick, E. M. , Koller, R., and Dhar, R., 1983, ras-Related gene sequences identified and isolated from S. cerevisiae. Nature (London), 306:707–709.CrossRefGoogle Scholar
  5. Dietzel, D., and Kurjan, J., 1987, The yeast SCG1 gene: a Ga-like protein implicated in the a- and a-factor response pathway. Cell, 50:1001–1010.PubMedCrossRefGoogle Scholar
  6. Fong, H. K. W., Yoshimoto, K. K., Eversole-Cire, P., and Simon, M. I., 1988, Identification of a GTP-binding protein a subunit that lacks an apparent ADP-ribosylation site for pertussis toxin. Proc. Natl. Acad. Sci. USA, 85:3066–3070.PubMedCrossRefGoogle Scholar
  7. Fukui, Y., and Kaziro, Y., 1985, Molecular cloning and sequence analysis of a ras gene from Schizosaccharomyces pombe. EMBO J., 4:687–691.PubMedGoogle Scholar
  8. Gilman, A. G., 1987, G proteins: transducers of receptor-generated signals. Annu. Rev. Biochem., 56:615–649.PubMedCrossRefGoogle Scholar
  9. Hurley, J. B., Simon, M. I., Teplow, D. B., Robishaw, J. D., and Gilman, A. G., 1984, Homologies between signal transducing G proteins and ras gene products. Science, 226:860–862.PubMedCrossRefGoogle Scholar
  10. Itoh, H., Katada, T., Ui, M., Kawasaki, H., Suzuki, K., and Kaziro, Y., 1988a, Identification of three pertussis toxin substrates (41, 40 and 39 kDa proteins) in mammalian brain. FEBS Lett., 230:85–89.PubMedCrossRefGoogle Scholar
  11. Itoh, H., Kozasa, T., Nagata, S., Nakamura, S., Katada, T., Ui, M., Iwai, S., Ohtsuka, E., Kawasaki, H., Suzuki, K., and Kaziro, Y., 1986, Molecular cloning and sequence determination of cDNAs for a subunit of the guanine nucleotide-binding proteins Gs, Gi, and Go from rat brain. Proc. Natl. Acad. Sci. USA, 83:3776–3780.PubMedCrossRefGoogle Scholar
  12. Itoh, H., Toyama, R., Kozasa, T., Tsukamoto, T., Matsuoka, M., and Kaziro, Y., 1988b, Presence of three distinct molecular species of Gi protein a subunit. J. Biol. Chem., 263:6656–6664.PubMedGoogle Scholar
  13. Jones, D. T., and Reed, R. R., 1987, Molecular cloning of five GTP-binding protein cDNA species from rat olfactory neuroepithelium. J. Biol. Chem., 262:14241–14249.PubMedGoogle Scholar
  14. Jurnak, F., 1985, Structure of the GDP domain of EF-Tu and location of the amino acids homologous to ras oncogene proteins. Science, 230:32–36.PubMedCrossRefGoogle Scholar
  15. Kahn, R. A., and Gilman, A. G., 1984, ADP-Ribosylation of Gs promoters the dissociation of its a and ß subunits. J. Biol. Chem., 259:6235–6240.PubMedGoogle Scholar
  16. Kaziro, Y., 1978, The role of guanosine 5’-triphosphate in polypeptide chain elongation. Biochim. Biophys. Acta, 505:95–127.PubMedCrossRefGoogle Scholar
  17. Kaziro, Y, Itoh, H., Kozasa, T., Toyama, R., Tsukamoto, T., Matsuoka, M., Nakafuku, M., Obara, T., Takagi, T., and Hernandez, R., 1988, Structure of the genes coding for G protein a subunits from mammalian and yeast cells. Cold Spring Harbor Symposia for Quantitative Biology, 53. (in press)Google Scholar
  18. Kozasa, T., Itoh, H., Tsukamoto, T., and Kaziro, Y., 1988, Isolation and characterization of human Gsa gene. Proc. Natl. Acad. Sci. USA, 85:2081–2085.PubMedCrossRefGoogle Scholar
  19. Lerea, C. L., Somers, D. E., Hurley, J. B., Klock, I. B., and Bunt-Milam, A. H., 1986, Identification of specific transducin a subunits in retinal rod and cone photoreceptors. Science, 324:77–80.CrossRefGoogle Scholar
  20. Lochrie, M. A., Hurley, J. B., and Simon, M. I., 1985, Sequence of the alpha subunit of photoreceptor G protein: homologies between transducin, ras, and elongation factors. Science, 228:96–99.PubMedCrossRefGoogle Scholar
  21. Masters, S. B., Stroud, R. M., and Bourne, H. R., 1986, Family of G protein a chains: amphipathic analysis and predicted structure of functional domains. Protein Eng., 1:47–54.PubMedCrossRefGoogle Scholar
  22. Matsuoka, M., Itoh, H., Kozasa, T., and Kaziro, Y., 1988, Sequence analysis of cDNA and genomic DNA for a putative pertussis toxin-insensitive guanine nucleotide-binding regulatory protein a subunit. Proc. Natl. Acad. Sci. USA, 85:5384–5388.PubMedCrossRefGoogle Scholar
  23. Mattera, R. , Codina, J., Crozat, A., Kidd, V., Woo, S. L. C., and Birnbaumer, L., 1986, Identification by molecular cloning of two forms of the ot-subunit of the human liver stimulatory (Gs) regulatory component of adenylate cyclase. FEBS Lett., 206:36–41.PubMedCrossRefGoogle Scholar
  24. Medynsky, D. C., Sullivan, K., Smith, D., Van Dop, C., Chang, F.-H., Fung, B. K.-K., Seeburg, P. H., and Bourne, H. R., 1985, Amino acid sequence of the α subunit of transducin deduced from the cDNA sequence. Proc. Natl. Acad. Sci. USA, 82:4311–4315CrossRefGoogle Scholar
  25. Miyajima, I., Nakafuku, M. , Nakayama, N., Brenner, C., Miyajima, A., Kaibuchi, K., Arai, K., Kaziro, Y., and Matsumoto, K., 1987, Cell, 50:1011–1019.PubMedCrossRefGoogle Scholar
  26. Nakafuku, M., Itoh, H., Nakamura S., and Kaziro, Y., 1987, Occurence in Saccharomyces cerevisiae of a gene homologous to the cDNA coding for the a subunit of mammalian G proteins. Proc. Natl. Acad. Sci. USA, 84:2140–2144.PubMedCrossRefGoogle Scholar
  27. Nakafuku, M., Obara, T., Kaibuchi, K., Miyajima, I., Miyajima, A., Itoh, H., Nakamura, S., Arai, K., Matsumoto, K., and Kaziro, Y., 1988, Isolation of a second yeast Saccharomyces cerevisiae gene (GPA2) coding for guanine nucleotide-binding regulatory protein: studies on its structure and possible functions. Proc. Natl. Acad. Sci. USA, 85:1374–1378.PubMedCrossRefGoogle Scholar
  28. Northup, J. K., Sternweise, P. C., Smigel, M. D., Shleifer, L. S., Ross, E. M., and Gilman, A. G., 1980, Purification of the regulatory component of adenylate cyclase. Proc. Natl. Acad. Sci. USA, 77:6516–6520.PubMedCrossRefGoogle Scholar
  29. Powers, S., Kataoka, T., Fasano, O., Goldfarb, M., Strathern, J., Broach, J., and Wigler, M., 1984, Genes in S. cerevisiae encoding proteins with domains homologous to the mammalian ras proteins. Cell, 36:607–612.PubMedCrossRefGoogle Scholar
  30. Robishaw, J. D., Russell, D. W., Harris, B. A., Smigel, M. D., and Gilman, A. G., 1986a, Deduced primary structure of the a subunit of the GTP-binding stimulatory protein of adenylate cyclase. Proc. Natl. Acad. Sci. USA, 83:1251–1255.PubMedCrossRefGoogle Scholar
  31. Robishaw, J. D., Smigel, M. D., and Gilman, A. G., 1986b, Molecular basis for two forms of the G protein that stimulates adenylate cyclase. J. Biol. Chem., 261:9587–9590.PubMedGoogle Scholar
  32. Sewell, J. L., and Kahn, R. A., 1988, Sequences of the bovine and yeast ADP-ribosylation factor and comparison to other GTP-binding proteins. Proc. Natl. Acad. Sci. USA, 85:4620–4624.PubMedCrossRefGoogle Scholar
  33. Stryer, L., and Bourne, H. R., 1986, G proteins: a family of signal transducers. Annu. Rev. Cell Biol., 2:391.PubMedCrossRefGoogle Scholar
  34. Sullivan, K. A., Miller, R. T., Masters, S. B., Beiderman, B., Heideman, W., and Bourne, H. R., 1987, Identification of receptor contact site involved in receptor — G protein coupling. Nature (London), 330:758–760.CrossRefGoogle Scholar
  35. Tanabe, T., Nukada, T., Nishikawa, Y., Sugimoto, K., Suzuki, H., Takahashi, H., Noda, M., Haga, T., Ichiyama, A., Kangawa, K., Minamino, N., Matsuo, H., and Numa, S., 1985, Primary structure of the a-subunit of transducin and its relationship to ras proteins. Nature (London), 315:242–245.CrossRefGoogle Scholar
  36. Toda, T., Uno, I., Ishikawa, T., Powers, S., Kataoka, T., Broek, D., Cameron, S., Broach, J., Matsumoto K., and Wigler, M., 1985, In yeast, RAS proteins are controlling elements of adenylate cyclase. Cell, 40:27–36.PubMedCrossRefGoogle Scholar
  37. Van Dop, C., Tsubokawa, M. , Bourne, H. R., and Ramachandran, J., 1984, Amino acid sequences of retinal transducin at the site ADP-ribosylated by cholera toxin. J. Biol. Chem., 259:696–698.PubMedGoogle Scholar
  38. Yatsunami, K., and Khorana, H. G., 1985, GTPase of bovine rod outer segments: the amino acid sequence of the a subunit as derived from the cDNA sequence. Proc. Natl. Acad. Sci. USA, 82:4316–4320.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Yoshito Kaziro
    • 1
  1. 1.Institute of Medical ScienceUniversity of TokyoTokyo 108Japan

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