Mutations at the RAS2 Locus that, in a ras1- Background, Impair the Growth of Yeast on Nonfermentable Carbon Sources
The proteins encoded by the RAS1 and RAS2 genes (collectively denominated RAS) are important for progression of S. cerevisiae through the cell cycle. In fact, yeast cells in which the function of the RAS2 protein is constitutively activated by a point mutation cannot properly arrest in Gl (Toda et al., 1985). On the other hand, cells with a disrupted RAS1 gene and with a temperature-sensitive RAS2 gene product are found prevalently as unbudded, G1-arrested cells upon temperature shift-up (De Vendittis et al., 1986).
KeywordsYeast Strain Adenylate Cyclase Adenylate Cyclase Activity RAS2 Protein Complete Synthetic Medium
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