Characterization of Elongation Factor Tu from Bacillus Subtilis Modified by Affinity Labelling

  • Jiří Jonák
  • Karel Karas
  • Ivan Rychlík

Abstract

The ability of aminoacyl-tRNA to complex with elongation factor Tu is essential since the ternary complex EF-Tu.GTP.aminoacyl-tRNA is the way aminoacyl-tRNA is carried to the A site of mRNA-programmed ribosomes. During the reaction, GTP in the complex is hydrolysed to GDP, and a stable form of EF-Tu.GDP, which has a low affinity for aminoacyl-tRNA, is released from the ribosome (Kaziro, 1978). The role of GTP might be described as that of an effector which controls the function of the protein binding site for aminoacyl-tRNA.

Keywords

Amino Acid Composition Elongation Factor Protein Binding Site Relative Molecular Mass Cysteic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Jiří Jonák
    • 1
  • Karel Karas
    • 1
  • Ivan Rychlík
    • 1
  1. 1.Institute of Molecular GeneticsCzechoslovak Acad. Sci.Prague 6Czechoslovakia

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