Characterization of Elongation Factor Tu from Bacillus Subtilis Modified by Affinity Labelling
The ability of aminoacyl-tRNA to complex with elongation factor Tu is essential since the ternary complex EF-Tu.GTP.aminoacyl-tRNA is the way aminoacyl-tRNA is carried to the A site of mRNA-programmed ribosomes. During the reaction, GTP in the complex is hydrolysed to GDP, and a stable form of EF-Tu.GDP, which has a low affinity for aminoacyl-tRNA, is released from the ribosome (Kaziro, 1978). The role of GTP might be described as that of an effector which controls the function of the protein binding site for aminoacyl-tRNA.
KeywordsAmino Acid Composition Elongation Factor Protein Binding Site Relative Molecular Mass Cysteic Acid
Unable to display preview. Download preview PDF.