Repair and Recombination of DNA Molecules

  • Edward A. Birge
Part of the Springer Series in Microbiology book series (SSMIC)


Every organism has mechanisms for maintaining the integrity of its nucleic acid (i.e., for repairing any damage). Nevertheless, most organisms, including even bacteria and viruses, exhibit some sort of genetic exchange. The two processes may seem antithetical, as recombination, the movement of genetic information from one molecule of nucleic acid to another, implies that the nucleic acid undergoes some kind of structural alteration. However, as is discussed in this chapter, many of the steps involved in completion of the recombination process are the same as those involved in repair, and recombination can be looked on as a process in which tree potential for damage to the nucleic acid is outweighed by the potential benefit to be derived from the new genetic information.


RecA Protein Strand Exchange Integration Host Factor LexA Protein Branch Migration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Claverys, J-P., Lacks, S.A. (1986). Heteroduplex DNA base mismatch repair in bacteria. Microbiological Reviews 50: 133–165.PubMedGoogle Scholar
  2. Derbyshire, K.M., Grindley, N.D.F. (1986). Replicative and conservative transposition in bacteria. Cell 47: 325–327.PubMedCrossRefGoogle Scholar
  3. Friedberg, E.C. (1987). The molecular biology of nucleotide excision repair of DNA: recent progress, pp. 1–23. In: Collins, A., Johnson, R.T., Boyle, J.M. (eds.) Molecular Biology of DNA Repair. Cambridge: Company of Biologists Limited.Google Scholar
  4. Gellert, M., Nash, H. (1987). Communication between segments of DNA during site-specific recombination. Nature 325: 401–414.PubMedCrossRefGoogle Scholar
  5. Kleckner, N. (1983). Transposon Tn10, pp. 261–298. In: Shapiro, J.A. (ed.) Mobile Genetic Elements. New York: Academic Press.Google Scholar
  6. Kowalczykowski, S.C. (1987). Mechanistic aspects of the DNA strand exchange activity of E. coli RecA protein. Trends in Biochemical Sciences 12: 141–145.CrossRefGoogle Scholar
  7. Lupski, J.R. (1987). Molecular mechanisms for transposition of drug-resistance genes and other movable genetic elements. Reviews of Infectious Diseases 9: 357368.Google Scholar
  8. Mizuuchi, K., Craigie, R. (1986). Mechanism of bacteriophage Mu transposition. Annual Review of Genetics 20: 385–429.PubMedCrossRefGoogle Scholar
  9. Radding, C.M. (1986). Homologous pairing & strand exchange mediated by RecA nucleoprotein filaments & networks, pp. 77–94. In: Gershowitz, H., Rucknagel, D.L., Tashian, R.E. (eds.) Evolutionary Perspectives and the New Genetics. New York: Liss.Google Scholar
  10. Radman, M., Wagner, R. (1986). Mismatch repair in Escherichia coli. Annual Review of Genetics 20: 523–538.PubMedCrossRefGoogle Scholar
  11. Amundsen, S.K., Taylor, A.F., Chaudhury, A.M., Smith, G.R. (1986). recD: the gene for an essential third subunit of exonuclease V. Proceedings of the National Academy of Sciences of the United States of America 83: 5558–5562.Google Scholar
  12. Barbè, J., Villaverde, A., Cairo, J., Guerrero, R. (1986). ATP hydrolysis during SOS induction in Escherichia coli. Journal of Bacteriology 167: 1055–1057.PubMedGoogle Scholar
  13. Bender, J., Kleckner, N. (1986). Genetic evidence that Tn10 transposes by a nonreplicative mechanism. Cell 45: 801–815.PubMedCrossRefGoogle Scholar
  14. Blanco, M., Herrera, G., Aleixandre, V. (1986). Different efficiency of UmuDC and MucAB proteins in UV light induced mutagenesis in Escherichia coli. Molecular and General Genetics 205: 234–239.PubMedCrossRefGoogle Scholar
  15. Chow, S.A., Honigberg, S.M., Bainton, R.J., Radding, C.M. (1986). Patterns of nuclease protection during strand exchange. recA protein forms heteroduplex DNA by binding to strands of the same polarity. The Journal of Biological Chemistry 261: 6961–6971.PubMedGoogle Scholar
  16. Ennis, D.G., Amundsen, S.K., Smith, G.R. (1987). Genetic functions promoting homologous recombination in Escherichia coli: a study of inversions in phage X. Genetics 115: 11–24.PubMedGoogle Scholar
  17. Fazakerley, G.V., Quignard, E., Woisard, A., Guschlbauer, W., van der Marel, G.A., van Boom, J.H., Jones, M., Radman, M. (1986). Structures of mismatched base pairs in DNA and their recognition by the Escherichia coli mismatch repair system. EMBO Journal 5: 3697–3703.PubMedGoogle Scholar
  18. Hatfull, G.F., Noble, S.M., Grindley, N.D.F. (1987). The gamma-delta resolvase induces an unusual DNA structure at the recombinational crossover point. Cell 49: 103–110.PubMedCrossRefGoogle Scholar
  19. Konforti, B.B., Davis, R.W. (1987). 3’ Homologous free ends are required for stable joint molecule formation by the RecA and single-stranded binding proteins of Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 84: 690–694.Google Scholar
  20. Lahue, R.S., Su, S-S., Modrich, P. (1987). Requirements for d(GATC) sequences in Escherichia coli mutHLS mismatch correction. Proceedings of the National Academy of Sciences of the United States of America 84: 1482–1486.PubMedCrossRefGoogle Scholar
  21. Lu, C., Scheuermann, R.H., Echols, H. (1986). Capacity of RecA protein to bind preferentially to UV lesions and inhibit the editing subunit (r) of DNA polymerase III: a possible mechanism for SOS-induced targeted mutagenesis. Proceedings of the National Academy of Sciences of the United States of America 83: 619623.Google Scholar
  22. Maxwell, A., Craigie, R., Mizuuchi, K. (1987). B protein of bacteriophage Mu is an ATPase that preferentially stimulates intermolecular DNA strand transfer. Proceedings of the National Academy of Sciences of the United States of America 84: 699–703.PubMedCrossRefGoogle Scholar
  23. Sargentini, N.J., Smith, K.C. (1986). Quantitation of the involvement of the recA, recB, recC, recF, recJ, recN, lexA, radA, radB, uvrD, and umuC genes in the repair of x-ray-induced DNA double-strand breaks in Escherichia coli. Radiation Research 107: 58–72.PubMedCrossRefGoogle Scholar
  24. Shen, M.M., Raleigh, E.A., Kleckner, N. (1987). Physical analysis of Tn10- and IS10-promoted transpositions and rearrangements. Genetics 116:359–369. Surette, M.G., Buch, S.J., Chaconas, G. (1987). Transpososomes: stable protein-DNA complexes involved in the in vitro transposition of bacteriophage Mu DNA. Cell 49: 253–262.Google Scholar
  25. Tessman, E.S., Tessman, I., Peterson, P.K., Forestal, J.D. (1986). Roles of RecA protease and recombinase activities of Escherichia coli in spontaneous and UV-induced mutagenesis and in Weigle repair. Journal of Bacteriology 168: 1159 1164.Google Scholar
  26. Thompson, J.F., Waechter-Brulla, D., Gumport, R.I., Gardner, J.F., Moitoso de Vargas, L., Landy, A. (1986). Mutations in an integration host factor-binding site: effect on lambda site-specific recombination and regulatory implications. Journal of Bacteriology 168: 1343–1351.PubMedGoogle Scholar
  27. Wang, T.V., Smith, K.C. (1986). recA (Srf) suppression of recF deficiency in the postreplication repair of UV-irradiated Escherichia coli K-12. Journal of Bacteriology 168: 940–946.Google Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • Edward A. Birge
    • 1
  1. 1.Department of MicrobiologyArizona State UniversityTempeUSA

Personalised recommendations