Abstract
This chapter is intended for a later stage in purification efforts, when a method has been established or published method has been tried but there may be some dissatisfaction with the overall procedure. The objectives of a scheme for the purification of an enzyme or other protein may be any or all of the following:
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1.
High overall recovery of activity
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2.
High degree of purity of the end product
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3.
Reproducibility
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(i)
within the laboratory
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(ii)
if scaled up or scaled down
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(iii)
in other laboratories
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(i)
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4.
Economical use of reagents and simple apparatus
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5.
Convenience in terms of working hours.
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© 1987 Springer Science+Business Media New York
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Scopes, R.K. (1987). Optimization of Procedures; Recombinant Protein Techniques. In: Protein Purification. Springer Advanced Texts in Chemistry. Springer, New York, NY. https://doi.org/10.1007/978-1-4757-1957-4_7
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DOI: https://doi.org/10.1007/978-1-4757-1957-4_7
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4757-1959-8
Online ISBN: 978-1-4757-1957-4
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