The Enzyme Purification Laboratory

  • Robert K. Scopes
Part of the Springer Advanced Texts in Chemistry book series (SATC)

Abstract

This chapter introduces the basic methods and equipment used in enzyme purification. It is entirely possible to purify enzymes using relatively simple equipment; the bare essentials might be considered a centrifuge and a UV spectrophotometer. Of course, access to a wider range of equipment and automated systems can make life much easier. But more elaborate equipment and procedures have their cost. It will be stressed many times in this book that delays during isolation of any enzyme must be avoided. Any procedure that relies on using equipment that is slow, unreliable, or sometimes unavailable is dangerous. By relying only on relatively simple and common apparatus, delays can usually be avoided. The message is clear: always use the simplest of procedures first, and use specialized methods only when all else has failed.

Keywords

Ammonium Sulfate Protein Solution Dialysis Tubing Enzyme Purification Barium Chloride 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • Robert K. Scopes
    • 1
  1. 1.Department of BiochemistryLa Trobe UniversityBundooraAustralia

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