Abstract
During pregnancy the syncytiotrophoblast layer of the human placenta elaborates both protein and steroid hormones (Gaspard et al., 1980; Simpson and MacDonald, 1981; Winkel et al., 1980). The mitotically inactive but biochemically functional syncytiotrophoblasts are formed in vivo by the fusion of the underlying mitotically active, mature, mononucleated cytotrophoblast cells (Boyd and Hamilton, 1967; Enders, 1965; Pierce and Midgley, 1963; Wislocki and Dempsey, 1955; Wynn, 1972).
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References
Boyd, J.D., and Hamilton,W.J. (1967) Development and structure of the human placenta from the end of the third month of gestation. J. Obstet. Gynecol. of Brit. Commonwealth 74, 161–226.
Carr, M.C. (1972) Study into the mechanisms of damage of human placental syncytiotrophoblast. ii. Histologic comparison of plasma-induced damage and specific enzyme-induced damage. Gynecol. Invest. 3, 203–214.
Carter, J.E. (1964) Morphologic evidence of syncytial formation from the cytotrophoblastic cells. Obstet. Gynecol. 23, 647–656.
Contractor, S.F. (1979) Receptors in cultured human trophoblast cells. In Protein Transmission Through Living Membranes, Hemmings, W.A. (ed.), Elsevier/North Holland Biomedical Press, Amsterdam, pp 3–75.
Contractor, S.F., Routledge, A. and Sooranna, S.R. (1984) Identification and estimation of cell types in mixed primary cell cultures of early and term human placenta. Placenta 5, 41–54.
Cotte, C., Easty, G.C., Neville, A.M., and Monaghan, P. (1980) Preparation of highly purified cytotrophoblast from human placenta with subsequent modulation to form syncytiotrophoblast in monolayer culture. In Vitro 16, 639–646.
Deal, C.L., and Guyda, H.J. (1983) Insulin receptors of human term placental cells and choriocarcinoma (JEG-3) cells: characteristics and regulation. Endocrinol. 112, 1512–1523.
de Ikonicoff, L.K. and Cedard, L. (1973) Localization of human chorionic gonadotropic and somatomammotropic hormones by the peroxidase immunohistoenzymologic method in villi and amniotic epithelium of human placentas (from six weeks to term). Am. J. Obstet. Gynecol. 116 1124–1132.
Enders, A.C. (1965) Formation of syncytium from cytotrophoblast in the human placenta. Obstet. Gynecol. 25, 378–386.
Foldes, J.J., and Schwartz, J. (1972) Some new aspects in human trophoblast cultures. Experientia 28,1468–1470.
Fox, H., and Kharkongor, F.N. (1970a) Morphology and enzyme histochemistry of cells derived from placental villi in tissue culture. J. Path. 101 267–276.
Fox, H.,and Kharkongor, F.N. (1970b) Immunofluorescent localization of chorionic gonadotrophin in the placenta and in tissue cultures of human trophoblast. J. Path. 101 277–282.
Gaspard, U.J., Hustin, J., Reuter, A.M., Lambotte, R., and Franchimont, P. (1980) Immunofluorescent localization of placental lactogen, chorionic gonadotropin and its alpha and beta subunits in organ cultures of human placenta. Placenta 1, 135–144.
Huot, R.I., Foidart, J.M. and Stromberg, K. (1979) Effects of culture conditions on the synthesis of human chorionic gonadotropin by placental organ cultures. In Vitro 15, 497–502.
Kaspi, T. and Nebel, L. (1974) Isolation of syncytiotrophoblast from human term placentas. Obstet. and Gynaecol. 43, 549–557.
Kawata, M., Parnes, J.R., and Herzenberg, L.A. (1984) Transcriptional control of HLA- A,B,C antigen in human placental cytotrophoblast isolated using trophoblast-and HLA-specific monoclonal antibodies and the fluorescence-activated cell sorter. J. Exp. Med. 160, 633–651.
Kliman, H.J., Nestler, J.E., Sermasi, E., Sanger, J.M. and Strauss, J.F. III (1986) Purification, characterization and in vitro differentiation of cytotrophoblasts from human term placentae. Endocrinol. 118, 1567–1582.
Kurman, R.J., Main, C.S., and Chen, H.C. (1984) Intermediate trophoblast: a distinctive form of trophoblast with specific morphological, biochemical and functional features. Placenta 5, 349–370.
Lobo, J.O., Bellino, F.L., and Bankert, L. (1985) Estrogen synthetase activity in human term placental cells in monolayer culture. Endocrinol. 116, 889–895.
Loke, Y.W. (1983) Human trophoblast in culture. In Biology of Trophoblast, Loke, Y.W. and Whyte, A. (eds.), Elsevier Science Publishers, Amsterdam, pp 663701.
Loke, Y.W., and Borland, R. (1970) Immunofluorescent localization of chorionic gonadotropin in monolayer cultures of human trophoblast cells. Nature 228, 561–562.
Loke, Y.W., Wilson, D.V. and Borland, R. (1972) Localization of human chorionic gonadotropin in monolayer cultures of trophoblast cells by mixed agglutination. Am. J. Obstet. Gynecol. 113, 875–879.
Martinez, F., Cheung, S.W., Crane, J.P. and Arias, F. (1983) Use of trophoblast cells in tissue culture for fetal chromosomal studies. Am. J. Obstet. Gynecal. 147, 542–547.
Nelson, D.M., Meister, R.K., Ortman-Nabi, J., Sparks, S. and Stevens, V.C. (1986) Differentiation and secretory activities of cultured human placental cytotrophoblasts. Placenta 7, 1–6.
Pierce, G.B. and Midgley, A.R. (1963) The origin and function of human syncytiotrophoblastic giant cells. Am. J. Pathol. 43, 153–172.
Simpson, E.R. and MacDonald, P.C. (1981) Endocrine physiology of the placenta. Ann. Rev. Physiol. 43, 163–188.
Stromberg, K., Azizkhan, J.C. and Speeg, K.V., Jr. (1978) Isolation of functional human trophoblast cells and their partial characterization in primary culture. In Vitro 14, 631–638.
Jogee, M., Myatt, L., Moore, P. and Elder, M.G. (1983) Prostacyclin production by human placental cells in short term culture. Placenta 4, 219–229.
Thiede, H.A. (1960) Studies of the human trophoblast in tissue culture. I. Cultural methods and histochemical staining. Am. J. Obstet. Gynecol. 79, 636–647.
Thiede, H.A., and Choate, J.W. (1963) Chorionic gonadotropin localization in the human placenta by immunofluorescent staining. II. Demonstration of hCG in the trophoblast and the amnion epithelium of immature and mature placentas. Obstet. Gynecol. 22, 433–443.
Winkel, C.A., Snyder, J.M., MacDonald, P.C. and Simpson, E.R. (1980) Regulation of cholesterol and progesterone synthesis in human placental cells in culture by serum lipoproteins. Endocrinol. 106,1054–1060.
Wislocki, G.B. and Dempsey, E.W. (1955) Electron microscopy of the human placenta. Anat. Rec. 123,133–149.
Wynn, R.M. (1972) Cytotrophoblastic specializations: An ultrastructual study of the human placenta. Am. J. Obstet. Gynecol. 114, 339–353.
Zeitler, P., Markoff, E. and Handwerger, S. (1983) Characterization of the synthesis and release of human placental lactogen and human chorionic gonadotropin by an enriched population of dispersed placental cells. J. Clin. Endocrinol. Metab. 57, 812–818.
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Lobo, J.O., Bellino, F.L., Siegel, A. (1987). Characterization of Isolated Cells in Primary Culture from Human term Placenta by Electron Microscopy and Immunohistochemistry. In: Miller, R.K., Thiede, H.A. (eds) Cellular Biology and Pharmacology of the Placenta. Trophoblast Research. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-1936-9_31
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DOI: https://doi.org/10.1007/978-1-4757-1936-9_31
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