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The Use of Radioimmunoassays for Cryptic Regions of Peptide Precursors, in the Study of Biosynthetic Mechanisms

  • R. Dimaline
  • H. Desmond
  • A.-C. Jonsson
  • S. Pauwels
  • H. Raybould
  • A. Varro
  • L. Vowles
  • G. J. Dockray
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 16 A)

Abstract

Elucidation of gene sequences encoding peptide hormones and neurotransmitters has allowed prediction of the primary amino acid sequences of all the peptides that are likely to be produced by biosynthetic processing of the precursors for these substances. Thus, in addition to the known biologically active sequences, the precursors demonstrably contain sequences of peptides (‘cryptic peptides’) whose identity and function are so far unknown. We have raised Ab’s to all the peptides expected to arise during biosynthesis of progastrin and proVIP, and to the C-terminal flanking peptide of CCK. All the Ab’s were produced by immunizing rabbits with small fragments or analogues of the precursor sequences, coupled to carrier protein in such a way as to leave the appropriate sequence free to promote Ab formation. We have used these Ab’s. in RIA to identify and characterize some novel peptides produced during gastrin and VIP biosynthesis, and to identify the cellular origins of the peptides by immunohistochemistry. The results have allowed elucidation of biosynthetic pathways and identification of cell-specific processing patterns. For progastrin at least, pathological changes in biosynthetic mechanisms have been identified.

Keywords

Basic Residue Biosynthetic Mechanism Synthetic Fragment Cryptic Region HPLC Elution Profile 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

Ab

antibody

CCK

cholecystokinin

VIP

vasoactive intestinal peptide

RIA

radioimmunoassay

PHI

peptide having N-terminal His and C-terminal Iln

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Copyright information

© Springer Science+Business Media New York 1986

Authors and Affiliations

  • R. Dimaline
    • 1
  • H. Desmond
    • 1
  • A.-C. Jonsson
    • 1
  • S. Pauwels
    • 1
  • H. Raybould
    • 1
  • A. Varro
    • 1
  • L. Vowles
    • 1
  • G. J. Dockray
    • 1
  1. 1.MRC Secretory Control Group, Physiological LaboratoryUniversity of LiverpoolLiverpoolUK

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