Photodiode Array HPLC Detectors in Metabolic Profiling and other Analytical Screening Techniques

  • Rokus A. de Zeeuw
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 16 A)


A new generation of UV-visible spectrophotometers has appeared in which a compound’s absorption spectrum is depicted in a fraction of a second by a multichannel photodiode array system. These detectors can record a series of spectra of very low intensity and store the data in their own memory and/or in external devices such as magnetic tapes or discs. These properties give diode array detectors (DAD’s) great potential as on-line detectors in HPLC.

We have evaluated two commercially available DAD’s for use in drug metabolite profiling and systematic toxicological analysis. The combined HPLC-DAD system proved to be very useful, comparable in potential to a GC-MS or LC-MS combination yet much cheaper. However, limitations became apparent, particularly in the area of data generation, storage and retrieval. Some of these may be inherent in the technique; others may be obviated when the technique is further developed.


Single Wavelength Minor Metabolite Human Bile Peak Apex Systematic Toxicological Analysis 
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  1. 1.
    Overzet, F., Ghijsen, R.T., Drenth, B.F.H. & de Zeeuw, R.A. (1982) J. Chromatog.240, 190–195.CrossRefGoogle Scholar
  2. 2.
    Overzet, F., Rurak, A., van der Voet, H., Drenth, B.F.H., Ghijsen, R.T. & de Zeeuw, R.A. (1983) J. Chromatog.267, 329–345.CrossRefGoogle Scholar
  3. 3.
    Overzet, F. & de Zeeuw, R.A. (1984) J. Pharm. Biomed. Anal.1, 3–17.CrossRefGoogle Scholar
  4. 4.
    Ghijsen, R.T., Drenth, B.F.H., Overzet, F. & de Zeeuw, R.A. (1982) J. High Resolu. Chromatog. Chromatog. Comm. 5, 192–198.CrossRefGoogle Scholar
  5. 5.
    Drouwen, A.C.J.H. (1985) Ph.D. Thesis, Delft, The Netherlands, pp. 71–86.Google Scholar
  6. 6.
    Daldrup, T., Susanto, F., Michalke, P. (1981) Z. Anal. Chem. 308, 413–427.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1986

Authors and Affiliations

  • Rokus A. de Zeeuw
    • 1
  1. 1.Department of Analytical Chemistry and ToxicologyState UniversityGroningenThe Netherlands

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