Detection, Identification and Quantitative Analysis of Drugs by 1H NMR
The application of high-resolution proton NMR spectroscopy to the measurement of drugs and their metabolites in biological fluids is of increasing interest to analytical chemists and toxicologists. Much of the pioneering work in this area has used very high magnetic field strengths (400 and 500 MHz). This article concerns the use of a 250 MHz NMR spectrophotometer in the detection and quantification of metabolites of the drug oxpentifylline in freeze-dried, reconstituted urine. The values obtained were within 10% of those from a specific HPLC method. The criteria necessary to obtain successful results using this technique are briefly discussed.
KeywordsChemical Noise High Magnetic Field Strength Aromatic Ring Proton Ring Proton Signal Internal Chemical Shift
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