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Determination of Benzodiazepines: The Present-Day Scene

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BIOACTIVE ANALYTES, Including CNS Drugs, Peptides, and Enantiomers

Part of the book series: Methodological Surveys in Biochemistry and Analysis ((MSBA,volume 16 A))

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Abstract

Methods that have been used for determining benzodiazepines in biological fluids include GC, HPLC, direct DPP, RIA and RRA methods*, of which GC, HPLC and RRA have proved the most valuable in clinical research on benzodiazepines. For low concentrations GC is particularly suitable, with ECB and possibly a SCOT column and a solids injector. Some hydroxylated benzodiazepines have to be derivatized. For thermally unstable compounds such as these, HPLC-UV is advantageous, although less sensitive than GC-ECD. HPLC with fluorescence detection is feasible but requires derivatization. HPLC with EC detection has so far been unpromising. HPLC could be useful for drug enantiomers.

RRA’s can detect both parent drug and pharmacologically active metabolites, preferably with assay on the sample direct. Especial attention is needed to receptor quality (brain-membrane preparation).

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Authors and Affiliations

  1. Center for Bio-Pharmaceutical Sciences, Division of Pharmacology, University of Leiden, P.O. Box 9503, 2300 RA, Leiden, The Netherlands

    M. Danhof, J. Dingemanse & D. D. Breimer

Authors
  1. M. Danhof
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  2. J. Dingemanse
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  3. D. D. Breimer
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Editor information

Editors and Affiliations

  1. Guildford Academic Associates, Guildford, UK

    Eric Reid

  2. ICI Pharmaceuticals Division, Alderley Park, UK

    Bryan Scales  & Ian D. Wilson  & 

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© 1986 Springer Science+Business Media New York

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Danhof, M., Dingemanse, J., Breimer, D.D. (1986). Determination of Benzodiazepines: The Present-Day Scene. In: Reid, E., Scales, B., Wilson, I.D. (eds) BIOACTIVE ANALYTES, Including CNS Drugs, Peptides, and Enantiomers. Methodological Surveys in Biochemistry and Analysis, vol 16 A. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-1892-8_12

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  • DOI: https://doi.org/10.1007/978-1-4757-1892-8_12

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-1894-2

  • Online ISBN: 978-1-4757-1892-8

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