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Assays of Peptidoglycans and Specific Antibodies in Biological Samples

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BIOACTIVE ANALYTES, Including CNS Drugs, Peptides, and Enantiomers

Part of the book series: Methodological Surveys in Biochemistry and Analysis ((MSBA,volume 16 A))

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Abstract

The presence of bacterial peptidoglycan fragments in mammals is apparently due to enzymic action on cell walls, yielding peptidogly-cans (PG’s) differing in size and chemical composition. The fragments can be manifested in the host by immunomodulating activity and an influence on sleep regulation. Antibodies (Ab’s) can be raised to soluble fragments of higher mol. wt., and have been detected in serum from patients with bacterial infections, particularly staphylococcal. Several sensitive immunoassays are now available for detecting soluble PG’s or specific Ab’s, to distinguish patients with the most serious infections and to complement other diagnostic methods. Consideration is given to methods for isolating, purifying and characterizing PG’s of low mol. wt., including HPLC and fast atom bombardment mass spectrometry (FAB-MS).

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Authors and Affiliations

  1. Institute of Immunology, P.O.B. 266, 41000, Zagreb, Yugoslavia

    Jelka Tomašić

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  1. Jelka Tomašić
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Editors and Affiliations

  1. Guildford Academic Associates, Guildford, UK

    Eric Reid

  2. ICI Pharmaceuticals Division, Alderley Park, UK

    Bryan Scales  & Ian D. Wilson  & 

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© 1986 Springer Science+Business Media New York

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Tomašić, J. (1986). Assays of Peptidoglycans and Specific Antibodies in Biological Samples. In: Reid, E., Scales, B., Wilson, I.D. (eds) BIOACTIVE ANALYTES, Including CNS Drugs, Peptides, and Enantiomers. Methodological Surveys in Biochemistry and Analysis, vol 16 A. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-1892-8_1

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  • DOI: https://doi.org/10.1007/978-1-4757-1892-8_1

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-1894-2

  • Online ISBN: 978-1-4757-1892-8

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