Biochemical Correlates of the Topographical Fate of Ligand-Receptor Complexes
The preceding chapters have focused on how to identify and characterize a specific cell surface receptor. Up to this point, the interaction between ligand and receptor has been conceptualized in a rather static framework, as if the receptor molecule remains unchanged in isolated membrane preparations or in detergent-solubilized solution, even after receptor occupancy. This may or may not be true. However, when ligand binding to intact cells is measured, a static conceptualization of the receptor population is almost certainly not valid. Not only is the cell surface membrane itself a dynamic medium but receptors are continually being synthesized, internalized, recycled, or degraded, so that the receptor population accessible for interaction with ligand at the cell surface is continually changing.
KeywordsCell Surface Receptor Radioligand Binding Luteinizing Hormone Release Hormone Polypeptide Hormone Receptor Population
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