Separation in Solution

  • Robert K. Scopes
Part of the Springer Advanced Texts in Chemistry book series (SATC)

Abstract

The methods described in Chapters 3 and 4 involve phase changes. The proteins pass from liquid phase (i.e., dissolved) to solid (precipitated or adsorbed) and back again. Not all proteins withstand the stresses occurring in these methods; gentler methods in which the proteins remain in solution at all times are available. One of these, gel filtration, is one of the principal techniques used in purifying enzymes and other proteins, and it will be considered in detail. Other methods, grouped together as electrophoretic techniques, are less widely used, for reasons which will be outlined in sections 5.2 and 5.3. A third, little-used method of separation in solution involves phase partitioning where proteins may move from one liquid phase into another (section 5.4). As a general technique, separation in solution is an important procedure both in research and industrial enzyme and protein purification.

Keywords

Isoelectric Point Elution Volume Gravitational Instability Separation Channel Discontinuous Buffer System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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General References

  1. L. Fischer (1980), Gel filtration chromatography. In Laboratory techniques in biochemistry and molecular biology (T. S. Work and R. H. Burdon, eds.), Vol. I, Part II, rev. ed., Elsevier/North-Holland, Amsterdam.Google Scholar
  2. A. H. Gordon (1979), Electrophoresis of proteins in Polyacrylamide and starch gels. In Laboratory techniques in biochemistry and molecular biology (T. S. Work and R. H. Burdon, eds.), Vol. I, Part I, rev. ed., Elsevier/North Holland, Amsterdam.Google Scholar
  3. Pharmacia Fine Chemicals AB Publications (1980), Gel filtration: Theory and practice. Uppsala.Google Scholar

Copyright information

© Springer Science+Business Media New York 1982

Authors and Affiliations

  • Robert K. Scopes
    • 1
  1. 1.Department of BiochemistryLa Trobe UniversityBundooraAustralia

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