The discovery of the process of conjugation in prokaryotes was due to one of the most fortuitous experimental designs in recent scientific history. Many other scientists had tried to demonstrate conjugation and had failed. Nevertheless, Lederberg and Tatum, working at Yale University, chose to make another attempt using a common laboratory strain of bacteria, Escherichia coli K-12, as their experimental system. They also decided to test only a few different isolates of the strain. Yet, by chance, they chose not only one of the few organisms which readily undergoes conjugation but also a fertile strain of that organism. In so doing they started an extremely fruitful branch of bacterial genetic research.
KeywordsDonor Cell Normal Fertility Recipient Cell Genetic Transfer Isolate From
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- Chater, K.F. 1979. Some recent developments in Streptomyces genetics, pp. 123–134. In: Sebek, O.K., Laskin, A.I. (eds.) Genetics of Industrial Microorganisms. Washington, D.C.: American Society for Microbiology.Google Scholar
- Chater, K.F., Merrick, M.J. 1979. Streptomyces, pp. 93–114. In: Parish, J.H. (ed.) Developmental Biology of Prokaryotes. Berkeley: University of California Press.Google Scholar
- Hopwood, D.A., Wright, H.M. 1976. Interactions of the plasmid SCP1 with the chromosome of Streptomyces coelicolor A3(2), pp. 607–619. In: Macdonald, K.D. (ed.) Second International Symposium on the Genetics of Industrial Microorganisms. New York: Academic Press.Google Scholar
- Jacob, F., Wollman, E.L. 1961. Sexuality and the Genetics of Bacteria. New York: Academic Press.Google Scholar
- Kusnierz, J.P., Lombaert, M.A. 1971. Etude de l’initiation du transfert chromosomique chez E. coli K-12. Comptes Rendues Hebdomadaires des Séances de l’Academie des Sciences, Série D 272:2844–2847.Google Scholar
- Nestman, E.R. 1979. Lethal zygosis in recombination-deficient mutants of E. coli. Canadian Journal of Genetics and Cytology 21:213–221.Google Scholar