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Dynamics of Macromolecular Motion

  • Chapter
Photon Correlation Spectroscopy and Velocimetry

Part of the book series: Nato Advanced Study Institutes Series ((NSSB,volume 23))

Abstract

One of the main areas of application of photon correlation techniques continues to be the study of the dynamics of macromolecular motion ie translational and rotational Brownian motions, including internal motions of flexible macromolecules. This topic has been reviewed in detail elsewhere (eg the books by Chu1 and Berne and Pecora2 and the articles by Cummins3 and Pusey4 in the proceedings of the last Capri institute). This article will contain a brief outline of the field with a more complete description of developments which have occurred in the last three years (up to mid-1976). References 1–4 can be consulted for further details concerning earlier developments. In section 2 we consider the motions of identical non-interacting particles. Section 2.1 is concerned with translational diffusion and the information which can be obtained from translational diffusion coefficients. In sections 2.2 and 2.3 we discuss briefly rotational and flexing motions. The effects and detection of sample polydispersity (non-identical particles) are considered in section 3 while section 4 is devoted to the effects of particle interactions. Perhaps the most common source of error in measurements in this whole area is the presence of particulate contaminants or “dust” in the solution under study. Section 5 contains a description of the effects of dust as well as a brief discussion of various methods which have been suggested for minimizing its deleterious effects. In the Appendix we present the results of a literature search covering the period 1973-mid 1976. The number of entries, nearly 200, emphasizes the wide-ranging activity in this field. Note that, besides this bibliography, there is a separate set of references applying to the text.

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References

  1. B Chu, Laser Light Scattering, (New York: Academic, 1974).

    Google Scholar 

  2. B J Berne and R Pecora, Dynamic Light Scattering, (New York: Wiley, 1976).

    Google Scholar 

  3. H Z Cummins in Photon Correlation and Light-Beating Spectroscopy, edited by H Z Cummins and E R Pike, (New York: Plenum, 1974).

    Google Scholar 

  4. P N Pusey in Photon Correlation and Light-Beating Spectroscopy, edited by H Z Cummins and E R Pike, (New York: Plenum, 1974).

    Google Scholar 

  5. P N Pusey, this volume.

    Google Scholar 

  6. J P Boon and A Bouiller, Phys Letters 55A, 391, 1976.

    ADS  Google Scholar 

  7. C Tanford, Physical Chemistry of Macromolecules, (New York: Wiley, 1961).

    Google Scholar 

  8. J Newman and H L Swinney, Biopolymers 15, 301, 1976.

    Article  Google Scholar 

  9. W N Huang and J E Frederick, Macromolecules 7, 34, 1974.

    Article  ADS  Google Scholar 

  10. J D G McAdam and T A King, Chem Phys 6, 109, 1974.

    Article  Google Scholar 

  11. P N Pusey, in Industrial Polymers: Characterization by Molecular Weight, edited by J H S Green and R Dietz (Transcripta Books, London, 1973) p 26.

    Google Scholar 

  12. S R Aragon and R Pecora, J Chem Phys 64, 2395 1976.

    Article  ADS  Google Scholar 

  13. T Tanaka, Polymer J 7, 62 1975.

    Article  Google Scholar 

  14. B J Ackerson, C M Sorensen, R C Mockler and W J O’Sullivan, Phys Rev Letters 34, 1371 1975.

    Article  ADS  Google Scholar 

  15. CM Sorensen, B J Ackerson, R C Mockler and W J O’Sullivan, Phys Rev A13, 1593 1976.

    ADS  Google Scholar 

  16. D Wonica, H L Swinney and H Z Cummins, Phys Rev Letters 37, 66 1976.

    Article  ADS  Google Scholar 

  17. R Pecora and Y Tagami, J Chem Phys 51, 3293, 1969.

    Article  ADS  Google Scholar 

  18. K S Schmitz and R Pecora, Biopolymers 14, 521 1975.

    Article  Google Scholar 

  19. S P Lee and B Chu, Appl Phys Lett 24, 261, 575 1974.

    Article  ADS  Google Scholar 

  20. S W Provencher, Biophys J 16, 27 1976.

    Article  Google Scholar 

  21. J H Goll and G B Stock (unpublished).

    Google Scholar 

  22. D E Koppel, J Chem Phys 57, 4814 1972.

    Article  ADS  Google Scholar 

  23. P N Pusey, D E Koppel, D W Schaefer, R D Camerini-Otero and S Koenig, Biochemistry 13, 952 1974.

    Article  Google Scholar 

  24. P N Pusey and J M Vaughan in Dielectric & Related Molecular Processes, Vol 12, edited by M Davies (The Chemical Society, London, 1975), p 48.

    Chapter  Google Scholar 

  25. J C Brown and P N Pusey, J Phys D 7, L31 1974.

    Article  ADS  Google Scholar 

  26. J C Brown, P N Pusey and R Dietz, J Chem Phys 62, 1136 1975.

    Article  ADS  Google Scholar 

  27. F C Chen, W Tscharnuter, D Schmidt and B Chu, J Chem Phys 60, 1675 1974.

    Article  ADS  Google Scholar 

  28. G A Brehm and V A Bloomfield, Macromolecules 8, 663 1975.

    Article  ADS  Google Scholar 

  29. J A Benbasat and V A Bloomfield, J Polymer Sci 10, 2475 1972.

    Google Scholar 

  30. H G Curme, private communication.

    Google Scholar 

  31. P I Rose, private communication.

    Google Scholar 

  32. J Newman, H L Swinney, S A Berkowitz and L A Day, Biochemistry 13, 4832, 1974.

    Article  Google Scholar 

  33. See, for example: M Adam, M Delsanti and G Jannink, J Phys Lett (France) 37, L53, 1976.

    Article  Google Scholar 

  34. D Bailey, T A King and D N Pinder, Chem Phys 12, 161, 1976.

    Article  Google Scholar 

  35. E Geissler and A M Hecht, J Chem Phys 65, 103, 1976.

    Article  ADS  Google Scholar 

  36. P N Pusey, J M Vaughan and G Williams, J Chem Soc Faraday Trans 2 70, 1696, 1974.

    Article  Google Scholar 

  37. P N Pusey, D W Schaefer, D E Koppel, R D Camerini-Otero and R M Franklin, J de Physique (Paris) 33, Cl–163, 1972.

    Google Scholar 

  38. D W Schaefer and B J Berne, Phys Rev Letters 32, 1110, 1974.

    Article  ADS  Google Scholar 

  39. J C Brown, P N Pusey, J W Goodwin and R H Ottewill, J Phys A 8, 664, 1975.

    Article  ADS  Google Scholar 

  40. P N Pusey, J Phys A 8, 1433, 1975.

    Article  ADS  Google Scholar 

  41. D W Schaefer and B J Ackerson, Phys Rev Letters 35, 1448, 1975.

    Article  ADS  Google Scholar 

  42. B J Ackerson, J Chem Phys 64, 242, 1976.

    Article  ADS  Google Scholar 

  43. P A Egelstaff, An Introduction to the Liquid State, (London: Academic, 1967).

    Google Scholar 

  44. Y Alon and A Hochberg, Rev Sci Instrum 46, 388, 1975.

    Article  ADS  Google Scholar 

Appendix — Bibliography

  1. B J Ackerson, 1976, “Correlations for interacting Brownian particles”, J Chem Phys 64, 242.

    Article  ADS  Google Scholar 

  2. M Adam, M Delsanti and G Jannink, 1976, “Light scattering by cooperative diffusion in semi-dilute polymer solutions”, J Phys Lett (France) 37, L53.

    Article  Google Scholar 

  3. A I Ahmed, F E Feeney, D T Osuga and Y Yeh, 1975, “Antifreeze glycoproteins from an Antarctic fish. Quasi-elastic light scattering studies of the hydrodynamic conformations of antifreeze glycoproteins”, J Biol Chem 250, 3344.

    Google Scholar 

  4. Y Alon and A Hochberg, 1975, “Improving light beating experiments by dust discrimination”, Rev Sci Instrum 46, 388.

    Article  ADS  Google Scholar 

  5. S S Alpert and G Banks, 1976, “The concentration dependence of the haemoglobin mutual diffusion coefficient”, Biophys Chem 4, 287.

    Article  Google Scholar 

  6. A R Altenberger and J M Deutch, 1973, “Light scattering from dilute macromolecular solutions”, J Chem Phys 59, 894.

    Article  ADS  Google Scholar 

  7. A R Altenberger, 1976, “Generalized diffusion processes and light scattering from a moderately concentrated solution of spherical macroparticles”, Chem Phys 15, 269.

    Article  ADS  Google Scholar 

  8. B Arnaud, J Legre and M Drifford, 1974, “Apparatus for spectral analysis of the laser light scattered from macromolecular solutions”, J Chim Phys Physicochim Biol 71, 591, (in French).

    Google Scholar 

  9. D Bailey, T A King and D N Pinder, 1976, “Polymer diffusion at intermediate concentrations studied by photon correlation spectroscopy”, Chem Phys (Netherlands) 12, 161.

    Article  Google Scholar 

  10. C B Bargeron, S M Cannon, R W Hart and R L McCally, 1973, “Light-beating spectrum of erythrocytes”, Phys Rev Lett 30, 205.

    Article  ADS  Google Scholar 

  11. C B Bargeron, 1973, “Analysis of intensity correlation spectra of mixtures of polystyrene latex spheres by least squares”, Appl Phys Lett 23, 379.

    Article  ADS  Google Scholar 

  12. C B Bargeron, 1974, “Measurement of a continuous distribution of spherical particles by intensity correlation spectroscopy. Analysis by cumulants”, J Chem Phys 61, 2134.

    Article  ADS  Google Scholar 

  13. C B Bargeron, 1974, “Analysis of intensity correlation spectra of mixtures of polystyrene latex spheres. Comparison of direct least squares fitting with the method of cumulants”, J Chem Phys 60, 2516.

    Article  ADS  Google Scholar 

  14. A R Bellamy and J D Harvey, 1976, “Biophysical studies of Reovirus type 3, III A laser light-scattering study of the RNA Transcriptase reaction”, Virology 70, 28.

    Article  Google Scholar 

  15. J A Benbasat and V A Bloomfield, 1973, “Inelastic light scattering study of macromolecular reaction kinetics, II Association reaction”, Macromolecules 6, 593.

    Article  ADS  Google Scholar 

  16. J A Benbasat and V A Bloomfield, 1975, “Joining of bacteriophage T4D heads and tails: A kinetic study by inelastic light scattering”, J Mol Biol 95, 335.

    Article  Google Scholar 

  17. A J Bennett and E E Uzgiris, 1973, “Laser doppler spectroscopy in an oscillating electric field”, Phys Rev A 8, 2662.

    Article  ADS  Google Scholar 

  18. N Ben-Yosef, O Ginio, D Mahlab and A Weitz, 1976, “Method for rapid measurement of particle size and relative number density of particles in suspension. I”, J Phys Chem 80, 253.

    Article  Google Scholar 

  19. B J Berne, 1973, “Chemical and biological applications of laser light scattering”, Accounts Chem Res 6, 318.

    Article  Google Scholar 

  20. B J Berne and R Giniger, 1973, “Electrophoretic light scattering as a probe of reaction kinetics”, Biopolymers 12, 1161.

    Article  Google Scholar 

  21. B J Berne and R Pecora, 1974, “Laser light scattering from liquids”, Ann Rev Phys Chem 25, 233.

    Article  ADS  Google Scholar 

  22. B J Berne and R Pecora, 1976, Dynamic light scattering, (New York: Wiley).

    Google Scholar 

  23. R J Blagrove, 1973, “Laser Rayleigh scattering from macromolecular solutions”, J Macromol Sci, Rev Macromol Chem 9, 71.

    Article  Google Scholar 

  24. V A Bloomfield, 1974, “Polymer normal mode analysis by inelastic light scattering”, Macromolecules 7, 846.

    Article  ADS  Google Scholar 

  25. V A Bloomfield and R J Mead, 1975, “Structure and stability of casein micelles”, J Dairy Science 58, 592.

    Article  Google Scholar 

  26. J P Boon and A Bouiller, 1976, “Experimental observation of long-time tails?”, Phys Letters 55A, 391.

    ADS  Google Scholar 

  27. G A Brehm and V A Bloomfield, 1975, “Analysis of polydispersity in polymer solutions by inelastic laser light scattering”, Macromolecules 8, 663.

    Article  ADS  Google Scholar 

  28. J C Brown and P N Pusey, 1974, “Measurement of diffusion coefficients of polydisperse solutes by photon correlation spectroscopy”, J Phys D (GB) 7, L31.

    Article  ADS  Google Scholar 

  29. J C Brown, P N Pusey and R Dietz, 1975, “Photon correlation study of polydisperse samples of polystyrene in cyclohexane”, J Chem Phys 62, 1136.

    Article  ADS  Google Scholar 

  30. J C Brown, P N Pusey, J W Goodwin and R H Ottewill, 1975, “Light scattering study of dynamic and time-averaged correlations in dispersions of charged particles”, J Phys A (GB) 8, 664.

    Article  ADS  Google Scholar 

  31. G Bueldt and G Meyerhoff, 1975, “Determination of diffusion coefficients of polymers in solutions up to moderate concentrations by inelastic light scattering”, Makromol Chem Suppl 1, 359 (in German).

    Article  Google Scholar 

  32. R D Camerini-Otero, P N Pusey, D E Koppel, D W Schaefer and R M Franklin, 1974, “Intensity fluctuation spectroscopy of laser light scattered by solutions of spherical viruses, R17, Q beta, BSV, PM2 and T7. II Diffusion coefficients, molecular weights, solvation and particle dimensions”, Biochemistry 13, 960.

    Article  Google Scholar 

  33. D S Cannell and S B Dubin, 1975, “Differential light scattering technique for the study of conformational changes in macromolecules and translational diffusion coefficients”, Rev Sci Instrum 46, 706.

    Article  ADS  Google Scholar 

  34. F D Carlson and A B Fraser, 1974, “Intensity fluctuation autocorrelation studies of the dynamics of muscular contraction”, in Photon correlation and light beating spectroscopy, Eds H Z Cummins and E R Pike (New York: Plenum), p 519.

    Google Scholar 

  35. F D Carlson and A B Fraser, 1974, “Dynamics of F-actin and F-actin complexes”, J Mol Biol 89, 273.

    Article  Google Scholar 

  36. F D Carlson, 1975, “The application of intensity fluctuation spectroscopy to molecular biology”, Ann Rev Biophys Bioeng 4, 243.

    Article  Google Scholar 

  37. A P Chaikovskii, A Y Khairullina and A P Ivanov, 1974, “Determination of polydisperse media parameters from intensity fluctuations of scattered radiation”, Opt and Spectrosc (USA) 36, 566.

    Google Scholar 

  38. F C Chen, W Tscharnuter, D Schmidt, B Chu and T Liu, 1974, “Measurement of diffusion coefficients of meningococcal polysaccharide by optical mixing spectroscopy, I Preliminary characterization on the aggregation of the group C polysaccharide”, Biopolymers 13, 2281.

    Article  Google Scholar 

  39. F C Chen, W Tscharnuter, D Schmidt and B Chu, 1974, “Experimental evaluation of macromolecular polydispersity in intensity correlation spectroscopy using the cumulant expansion technique”, J Chem Phys 60, 1675.

    Article  ADS  Google Scholar 

  40. F C Chen, A Chrzeszezyk and B Chu, 1976, “Quasielastic laser light scattering of phosphatidylcholine vesicles”, J Chem Phys 64, 3403.

    Article  ADS  Google Scholar 

  41. S H Chen and A V Nurmikko, 1974, “Photon correlation spectroscopy in biology”, in Spectroscopy in biology and chemistry — Neutron, X-ray, Laser, Eds S H Chen and S Yip (New York: Academic).

    Google Scholar 

  42. B Chu, 1974, “Laser Light scattering”, (New York: Academic).

    Google Scholar 

  43. B Chu, A Yeh, F C Chen and B Weiner, 1975, “Self association of beta-lactoglobulin A in acid solution, I Translational diffusion coefficients”, Biopolymers 14, 93.

    Article  Google Scholar 

  44. R J Cohen, J A Jedziniak and G B Benedek, 1975, “Study of the aggregation and allosteric control of bovine glutamate dehydrogenase by means of quasielastic light scattering spectroscopy”, Proc Roy Soc A 345, 73.

    Article  ADS  Google Scholar 

  45. R J Cohen and G B Benedek, 1975, “Immunoassay by light scattering spectroscopy”, Immunochemistry 12, 349.

    Article  Google Scholar 

  46. V G Cooper, S Yedgar and Y Barenholz, 1973, “Diffusion coefficients of mixed micelles of Triton X-100 and sphyngomyelin and of sonicated sphyngomyelin liposomes by autocorrelation spectroscopy of Rayleigh scattered light”, Biochim Biophys Acta 363, 86 and 98.

    Google Scholar 

  47. M Corti and V Degiorgio, 1975, “Light-scattering study on the micellar properties of a nonionic surfactant”, Optics Comm 14, 358.

    Article  ADS  Google Scholar 

  48. H Z Cummins and E R Pike, 1974, Photon correlation and light-beating spectroscopy, (New York: Plenum).

    Google Scholar 

  49. H Z Cummins, 1974, “Applications of light beating spectroscopy to biology”, in Photon correlation and light-beating spectroscopy, Eds H Z Cummins and E R Pike (New York: Plenum) p 285.

    Google Scholar 

  50. K J Czworniak and D R Jones, 1974, “Effect of a finite collection aperture on autocorrelation light-scattering spectroscopy”, J Opt Soc Am 64, 86.

    Article  ADS  Google Scholar 

  51. S K Davi, 1974, “Application of a laser self-beat spectroscopic technique to the study of solutions of human plasma lipoproteins”, J Chem Soc Faraday Trans II 70, 700.

    Article  Google Scholar 

  52. R W DeBlois, E E Uzgiris, S K Davi and A M Gotto, 1973, “Application of laser self-beat spectroscopic technique to the study of solutions of human plasma low-density lipoproteins”, Biochemistry 12, 2645.

    Article  Google Scholar 

  53. RK Dewan and V A Bloomfield, 1973, “Molecular weight of bovine milk casein micelles from diffusion and viscosity measurements”, J Dairy Sci 56, 66.

    Article  Google Scholar 

  54. P Doherty and G B Benedek, 1974, “Effect of electric charge on the diffusion of macromolecules”, J Chem Phys 61, 5426.

    Article  ADS  Google Scholar 

  55. M Drifford, D Massignon, B Arnaud, M Gilbert, R Menez and R Rousson, 1975, “Use of lasers in the study of solution dynamics”, Bull Inf Sci and Tech, Commis Energ At (Fr) 205, 5, (in French).

    Google Scholar 

  56. S B Dubin, G Feher and G B Benedek, 1973, “Study of the chemical denaturation of lysozyme by optical mixing spectroscopy”, Biochemistry 12, 714.

    Article  Google Scholar 

  57. S B Dubin and D S Cannell, 1975, “Effect of succinate on the translation diffusion coefficient of aspartate transcarbamylase”, Biochemistry 14, 192.

    Article  Google Scholar 

  58. D L Ermak and Y Yeh, 1974, “Equilibrium electrostatic effects on the behaviour of polyions in solution: Polyion-mobile ion interaction”, Chem Phys Lett 24, 243.

    Article  ADS  Google Scholar 

  59. DL Ermak, 1975, “A computer simulation of charged particles in solution, I Technique and equilibrium properties and II Polyion diffusion coefficient”, J Chem Phys 62, 4189 and 4197.

    Article  ADS  Google Scholar 

  60. J A Farrell, J D Harvey and A R Bellamy, 1974, “Biophysical studies of reovirus type 3. I The molecular weights of reovirus and reovirus cores”, Virology 62, 145.

    Article  Google Scholar 

  61. P A Fleury and J P Boon, 1973, “Laser light scattering in fluid systems”, Adv Chem Phys 24, 1.

    Article  Google Scholar 

  62. N C Ford, R Gabler and F E Karasz, 1973, “Self-beat spectroscopy and molecular weight”, Advan Chem Ser 125, 25.

    Article  Google Scholar 

  63. A B Fraser, E Eisenberg, W W Kielley and F D Carlson, 1975, “The interaction of heavy meromyosin and subfragment 1 with actin. Physical measurements in the presence and absence of adenosine triphosphate”, Biochemistry 14, 2207.

    Article  Google Scholar 

  64. L Friedhoff and B J Berne, 1976, “Irreversible thermodynamic analysis of electrophoretic light scattering experiments”, Biopolymers 15, 21.

    Article  Google Scholar 

  65. S Fujime, 1973, “Spectrum of light quasielastically scattered from coupled reaction systems of macromolecules”, Macromolecules 6, 361.

    Article  ADS  Google Scholar 

  66. S Fujime and M Maruyama, 1973, “Spectrum of light quasielastically scattered from linear macromolecules”, Macromolecules 6, 237.

    Article  ADS  Google Scholar 

  67. S Fujime, 1974, “Quasielastic light scattering. Its application to the study of biological macromolecules”, Seibutsu Butsuri 14, 9, (in Japanese).

    Article  Google Scholar 

  68. R Gabler, E W Westhead and N C Ford, 1974, “Studies of ribosomal diffusion coefficients using laser light scattering spectroscopy”, Biophys J 14, 528.

    Article  Google Scholar 

  69. R Gabler, N C Ford and E W Westhead, 1975, “Conformational changes of glyceraldehyde-3-phosphate dehydrogenase observed using laser light-scattering spectroscopy”, Biophys J 15, 747.

    Article  Google Scholar 

  70. M J Garvey, T F Tadros and B Vincent, 1976, “A comparison of the absorbed layer thickness obtained by several techniques of various molecular weight fractions of polyvinyl alcohol on aqueous polystyrene latex particles”, J Colloid Interface Sci 55, 440.

    Article  Google Scholar 

  71. E Geissler and A M Hecht, 1976, “Rayleigh light scattering in concentrated polymer solutions above the cloud point”, Chem Phys Letters 37, 343.

    Article  ADS  Google Scholar 

  72. E Geissler and A M Hecht, 1976, “Rayleigh light scattering from concentrated solutions of polystyrene in cyclohexane”, J Chem Phys 65, 103.

    Article  ADS  Google Scholar 

  73. D D. Haas, R V Mustacich, B A Smith and B R Ware, 1974, “Angular dependence of light scattering linewidths from haemoglobin solutions”, Biochem Biophys Res Comm 59, 174.

    Article  Google Scholar 

  74. F R Hallett and L A Gray, 1974, “Quasi-elastic light scattering studies of hyaluronic acid solutions”, Biochim Biophys Acta 343, 648.

    Article  Google Scholar 

  75. C C Han and H Yu, 1974, “Intramolecular chain dynamics by forward depolarized scattering”, J Chem Phys 61, 2650.

    Article  ADS  Google Scholar 

  76. S Harris, 1976, “Limiting law theory for light scattering in dispersions of charged spheres”, J Phys A (GB) 9, 1093.

    Article  ADS  Google Scholar 

  77. S L Hartford and W H Flygare, 1975, “Electrophoretic light scattering on calf thymus DNA and tobacco mosaic virus”, Macromolecules 8, 80.

    Article  ADS  Google Scholar 

  78. J D Harvey, 1973, “Diffusion coefficients and hydrodynamic radii of three spherical RNA viruses by laser light scattering”, Virology 56, 365.

    Article  Google Scholar 

  79. J D Harvey, J A Farrell and A R Bellamy, 1974, “Biophysical studies of reovirus type 3. II Properties of the hydrated particle”, Virology 62, 154.

    Article  Google Scholar 

  80. J D Harvey, D F Walls and M W Woolford, 1976, “Electrophoretic investigations by laser light scattering”, Optics Commun 18, 367.

    Article  ADS  Google Scholar 

  81. A Hochberg and W Low, 1976, “Determining particle sizes by homodyne spectroscopy of multiply scattered light”, J Appl Phys 47, 1001.

    Article  ADS  Google Scholar 

  82. L Hocker, J Krupp, G B Benedek and J Vournakis, 1973, “Observations of self-aggregation and dissociation of E coli ribosomes by optical mixing spectroscopy”, Biopolymers 12, 1677.

    Article  Google Scholar 

  83. C Holt, D G Dalgleish and T G Parker, 1973, “Particle size distributions in skim milk”, Biochim Biophys Acta 328, 428.

    Article  Google Scholar 

  84. C Holt, 1975, “Casein micelle size from elastic and quasielastic light scattering measurements”, Biochim Biophys Acta 400, 293.

    Article  Google Scholar 

  85. J L Holtzman, R R Erickson, T E Gram, R K Dewan and V A Bloomfield, 1973, “Kinetics and inelastic light-scattering studies of the role of phospholipids in the microsomal membrane structure”, Drug Metab Dispos 1, 74.

    Google Scholar 

  86. J L Holtzman, R R Erickson, R K Dewan and V A Bloomfield, 1973, “Inelastic laser light scattering and particle size of detergent treated microsomes”, Biochem Biophys Res Commun 52, 15.

    Article  Google Scholar 

  87. W N Huang and J E Frederick, 1973, “Rayleigh line spectrometry of very large macromolecules in dilute solution”, J Chem Phys 58, 4022.

    Article  ADS  Google Scholar 

  88. W N Huang and J E Frederick, 1974, “Determination of intramolecular motion in a random-coil polymer by means of quasielastic light scattering”, Macromolecules 7, 34.

    Article  ADS  Google Scholar 

  89. A M Jamieson and A G Walton, 1973, “Studies of viscous drag in binary liquid mixtures by quasielastic light scattering”, J Chem Phys 58, 1054.

    Article  ADS  Google Scholar 

  90. A M Jamieson and C T Presley, 1973, “Anisotropic translational diffusion in dilute aqueous solutions of partially hydrolyzed polyacrylamide by quasielastic light scattering”, Macromolecules 6, 358.

    Article  ADS  Google Scholar 

  91. A M Jamieson and A R Maret, 1973, “Quasielastic laser light scattering”, Chem Soc Rev 2, 325.

    Article  Google Scholar 

  92. A M Jamieson, T Y Lee and I A Schafer, 1974, “Structural studies of human placental dermatan sulfate during development using optical mixing spectroscopy”, Biopolymers 13, 2133.

    Article  Google Scholar 

  93. D Jolly and H Eisenberg, 1976, “Photon correlation spectroscopy, total intensity light scattering with laser radiation, and hydrodynamic studies of a well fractionated DNA sample”, Biopolymers 15, 61.

    Article  Google Scholar 

  94. J Josephowicz and F R Hallett, 1975, “Homodyne electrophoretic light scattering of polystyrene spheres by laser cross-beam intensity correlation”, Appl Opt 14, 740.

    Article  ADS  Google Scholar 

  95. M Jullien and B Arrio, 1975, “Quasielastic scattering of light. Application to biological problems”, Biochimie 57, 429, (in French).

    Article  Google Scholar 

  96. M Jullien and D Thusius, 1976, “Mechanism of Bovine Liver Glutamate dehydrogenase self-assembly. III Characterization of the association-dissociation stoichiometry with quasielastic light scattering”, J Mol Biol 101, 397.

    Article  Google Scholar 

  97. J H Kaplan and E E Uzgiris, 1975, “The detection of phytomitogen-induced changes in human lymphocyte surfaces by laser doppler spectroscopy”, J Immunol Methods 7, 337.

    Article  Google Scholar 

  98. T A King, A Knox and J D G McAdam, 1973, “Internal motion in chain polymers”, Chem Phys Letters 19, 351.

    Article  ADS  Google Scholar 

  99. T A King, A Knox, W I Lee and J D G McAdam, 1973, “Polymer translational diffusion, 1 Dilute theta solutions, polystyrene in cyclohexane”, Polymer 14, 151.

    Article  Google Scholar 

  100. T A King, A Knox and J D G McAdam, 1973, “Polymer translational diffusion, 2 Non theta solutions, polystyrene in 2-butanone”, Polymer 14, 293.

    Article  Google Scholar 

  101. T A King, A Knox and J D G McAdam, 1973, “Translational and rotational diffusion of Tobacco mosaic virus from polarized and depolarized light scattering”, Biopolymers 12, 1917.

    Article  Google Scholar 

  102. T A King, A Knox and J D G McAdam, 1974, “Polymer dynamics in solutions and gels from Rayleigh light-scattered line widths”, J Polym Sci Polym Symp 44, 195.

    Article  Google Scholar 

  103. D E Koppel, 1974, “Study of E coli ribosomes by intensity fluctuation spectroscopy of scattered laser light”, Biochemistry 13, 2712.

    Article  Google Scholar 

  104. S P Lee and B Chu, 1974, “Least-squares integration method in intensity fluctuation spectroscopy of macromolecular solutions with bimodal distributions”, Appl Phys Lett 24, 261.

    Article  ADS  Google Scholar 

  105. S P Lee and B Chu, 1974, “Application of least-squares (difference-integration) method to cumulants analysis in intensity fluctuation spectroscopy”, Appl Phys Lett 24, 575.

    Article  ADS  Google Scholar 

  106. W I Lee and J M Schurr, 1973, “Intensity autocorrelation function for a flexible polymer”, Chem Phys Lett 23, 603.

    Article  ADS  Google Scholar 

  107. W I Lee and J M Schurr, 1974, “Dynamic light scattering studies of poly-L-lysine hydrogen bromide in the presence of added salt”, Biopolymers 13, 903.

    Article  Google Scholar 

  108. W I Lee and J M Schurr, 1975, “Laser light scattering studies of poly-L-lysine hydrobromide in aqueous solutions”, J Polym Sci, Polym Phys Ed 13, 873.

    Article  Google Scholar 

  109. W I Lee and J M Schurr, 1976, “Effect of long-range hydrodynamic and direct intermacromolecular forces on translational diffusion”, Chem Phys Letters 38, 71.

    Article  ADS  Google Scholar 

  110. M A Loewenstein and M H Birnboim, 1975, “Method for measuring sedimentation and diffusion of macromolecules in capillary tubes by total intensity and quasielastic light-scattering techniques”, Biopolymers 14, 419.

    Article  Google Scholar 

  111. I Lundstroem and D McQueen, 1974, “Inelastic light scattering from the free surfaces of viscoelastic polymer solutions”, J Chem Soc, Faraday Trans I 70, 2351.

    Article  Google Scholar 

  112. K B Lyons, R C Mockler and W J O’Sullivan, 1973, “Light-scattering investigation of Brownian motion in a critical mixture”, Phys Rev Lett 30, 42.

    Article  ADS  Google Scholar 

  113. K B Lyons, R C Mockler and W J O’Sullivan, 1973, “K-dependent Brownian diffusion constant in a critical mixture”, Phys Fluids 16, 2092.

    Article  ADS  Google Scholar 

  114. K B Lyons, R C Mockler and W J O’Sullivan, 1974, “Brownian motion in a critical mixture. K-dependent diffusion”, Phys Rev A 10, 393.

    Article  ADS  Google Scholar 

  115. H Maeda and N Saito, 1973, “Spectral distribution of the light scattered from rodlike macromolecules in solution. II The effect of optical anisotropy”, Polymer J 4, 309.

    Article  Google Scholar 

  116. T Maeda, S Ishiwata and S Fujime, 1974, “Light-beating study of the effect of beta-actinin on the interaction between F-actin and heavy meromyosin”, Biochim Biophys Acta 336, 445.

    Article  Google Scholar 

  117. N A Mazer, G B Benedek and M Carey, 1976, “An investigation of the micellar phase of sodium dodecyl sulphate in aqueous sodium chloride solutions using quasielastic light scattering spectroscopy”, J Phys Chem 80, 1075.

    Article  Google Scholar 

  118. J D G McAdam and T A King, 1974, “Polymer dynamics in solution from Rayleigh line profile spectroscopy”, Chem Phys 6, 109.

    Article  Google Scholar 

  119. J D G McAdam, T A King and A Knox, 1974, “Molecular motion in polymer networks and concentrated solutions from photon correlation spectroscopy”, Chem Phys Lett 26, 64.

    Article  ADS  Google Scholar 

  120. J D G McAdam and T A King, 1974, “Dynamics of long chain polymers from intensity fluctuation spectroscopy”, Chem Phys Letters 28, 90.

    Article  ADS  Google Scholar 

  121. M E McDonnell and A M Jamieson, 1976, “Rapid characterization of protein molecular weights and hydrodynamic structures by quasielastic laser light scattering”, Biopolymers 15, 1283.

    Article  Google Scholar 

  122. C T Meneely, D F Edwards and R L Rimsay, 1975, “Diffusion constant measurements of macromolecules in solutions containing particulate components”, Appl Optics 14, 2129.

    Article  ADS  Google Scholar 

  123. R Menez, B Arnaud and M Drifford, 1975, “Spectral distribution of the light scattered from charged macromolecules in solution under the influence of an electric field”, C R Hebd Seances Acad Sci Ser C 280, 157 (in French).

    Google Scholar 

  124. G Meyerhoff and G Bueldt, 1974, “Inelastic light scattering of cellulose nitrate solutions”, Makromol Chem 175, 675, (in German).

    Article  Google Scholar 

  125. K Mishima and O Sugano, 1976, “Light scattering of lecithin vesicles in salt solution I”, J Phys Soc Japan 40, 1130.

    Article  ADS  Google Scholar 

  126. R Mohan, R Steiner and R Kaufmann, 1976, “Laser doppler spectroscopy as applied to electrophoresis in protein solutions”, Analyt Biochem 70, 506.

    Article  Google Scholar 

  127. C V Morr, S H C Lin, R K Dewan, and V A Bloomfield, 1973, “Molecular weights of fractionated bovine milk casein micelles from analytical ultracentrifugation and diffusion measure-ments”, J Dairy Sci 56, 415.

    Article  Google Scholar 

  128. J P Munch, S Candau, R Duplessix, C Picot and H Benoit, 1974, “Study of viscoelastic properties of model networks by inelastic light scattering”, J Phys (Paris) Lett 35, L239, (in French).

    Article  Google Scholar 

  129. J P Munch, S Candau, R C Duplessix, C Picot, J Herz and H Benoit, 1976, “Spectrum of light scattered from viscoelastic gels”, J Polym Sci Polym Phys Ed 14, 1097.

    Article  Google Scholar 

  130. J Newman, H L Swinney, S A Berkowitz and L A Day, 1974, “Hydrodynamic properties and molecular weight of fd bacteriophage DNA”, Biochemistry 13, 4832.

    Article  Google Scholar 

  131. T Olson, M J Fournier, K H Langley and N C Ford, 1976, “Detection of a major conformational change in tRNA by laser light scattering”, J Mol Biol 102, 193.

    Article  Google Scholar 

  132. A Perico, P Piaggio and C Cuniberti, 1975, “Dynamics of chain molecules. II Spectral distribution of the light scattered from flexible macromolecules”, J Chem Phys 62, 2690.

    Article  ADS  Google Scholar 

  133. D C Petersen and R A Cone, 1975, “The electric dipole moment of rhodopsin solubilized in Triton X-100”, Biophys J 15, 1181.

    Article  Google Scholar 

  134. G D J Phillies, 1973, “Effects of intermacromolecular interactions diffusion. Ill Electrophoresis in three-component solutions”, J Chem Phys 59, 2613.

    Article  ADS  Google Scholar 

  135. G D J Phillies, 1974, “Effects of intermacromolecular interaction on diffusion. I Two-component solutions”, J Chem Phys 60, 976.

    Article  ADS  Google Scholar 

  136. G D J Phillies, 1974, “Effects of intermacromolecular interactions on diffusion. II Three-component solutions”, J Chem Phys 60, 983.

    Article  ADS  Google Scholar 

  137. G D J Phillies, 1974, “Excess chemical potential of dilute solutions of spherical polyelectrolytes”, J Chem Phys 60, 2721.

    Article  ADS  Google Scholar 

  138. G D J Phillies, 1975, “Fluorescence correlation spectroscopy and nonideal solutions”, Biopolymers 14, 499.

    Article  Google Scholar 

  139. G D J Phillies, 1975, “Continuum hydrodynamic interactions and diffusion”, J Chem Phys 62, 3925.

    Article  ADS  Google Scholar 

  140. G D J Phillies, 1976, “Contribution of slow charge fluctuations to light scattering from a monodisperse solution of macromolecules”, Macromolecules 9, 447.

    Article  ADS  Google Scholar 

  141. C Price, J D G McAdam, T P Lally and D Woods, 1974, “Determination of the molecular weight and hydrodynamic dimensions of micelles formed from a block copolymer”, Polymer 15, 228.

    Article  Google Scholar 

  142. P N Pusey, 1973, “Measurement of diffusion coefficients of polydisperse solutes by intensity fluctuation spectroscopy”, in Industrial polymers: Characterization by molecular weight, Eds J H S Green and R Dietz, (London: Transcripta Books), p 26.

    Google Scholar 

  143. P N Pusey, D E Koppel, D W Schaefer, R D Camerini-Otero and S H Koenig, 1974, “Intensity fluctuation spectroscopy of laser light scattered by solutions of spherical viruses, R17, Q beta, BSV, PM2 and T7. I Light scattering technique”, Biochemistry 13, 952.

    Article  Google Scholar 

  144. P N Pusey, 1974, “Macromolecular diffusion”, in Photon correlation and light-beating spectroscopy, Eds H Z Cummins and E R Pike (New York: Plenum), p 387.

    Google Scholar 

  145. P N Pusey, J M Vaughan and G Williams, 1974, “Diffusion of polystyrene in solution studied by photon correlation spectroscopy”, J Chem Soc, Faraday Trans 2 70, 1696.

    Article  Google Scholar 

  146. P N Pusey and J M Vaughan, 1975, “Light scattering and intensity fluctuation spectroscopy”, in Dielectric and related molecular processes, Ed M Davies (London: The Chemical Society) p 48.

    Chapter  Google Scholar 

  147. P N Pusey, 1975, “The dynamics of interacting Brownian particles”, J Phys A (GB) 8, 1433.

    Article  ADS  Google Scholar 

  148. T Raj and W H Flygare, 1974, “Diffusion studies of BSA by quasielastic light scattering”, Biochemistry 13, 3336.

    Article  Google Scholar 

  149. L Rimai, I Salmeen, D Hart, L Liebes, M A Rich and J J McCormick, 1975, “Electrophoretic mobilities of RNA tumor viruses. Studies by Doppler-shifted light scattering spectroscopy”, Biochemistry 14, 4621.

    Article  Google Scholar 

  150. B E A Saleh and J Hendrix, 1976, “The correlation function profile of light scattered from polydisperse large random coil macromolecules in dilute solutions”, Chem Phys 12, 25.

    Article  Google Scholar 

  151. I Salmeen, L Rimai, L Liebes, M A Rich and J J McCormick, 1975, “Hydrodynamic diameters of RNA tumor viruses. Studies by laser beat frequency light scattering spectroscopy of avian myeloblastosis and Rauscher murine leukemia viruses”, Biochemistry 14, 134.

    Article  Google Scholar 

  152. M J Stephen, 1974, “Doppler shifts in light scattering from macroions in solution”, J Chem Phys 61, 1598.

    Article  ADS  Google Scholar 

  153. T Tanaka, L O Hocker and G B Benedek, 1973, “Spectrum of light scattered from a viscoelastic gel”, J Chem Phys 59, 5151.

    Article  ADS  Google Scholar 

  154. T Tanaka and G B Benedek, 1975, “Observation of protein diffusivity in intact human and bovine lenses with application to cataract”, Investigative Ophthalmology 14, 449.

    Google Scholar 

  155. T Tanaka, 1974, “Quasielastic light scattering from polydisperse polymer solution”, J Phys Soc Japan 37, 575.

    Article  ADS  Google Scholar 

  156. T Tanaka, 1975, “Quasielastic light scattering from polydisperse polymer solutions”, Polym J 7, 62.

    Article  Google Scholar 

  157. E E Uzgiris and F M Costaschuk, 1973, “Investigation of colloid stability in polyelectrolyte solutions by laser Doppler spectroscopy”, Nature, Phys Sci 242, 77.

    ADS  Google Scholar 

  158. E E Uzgiris, 1973, “Characterization of thin films by optical mixing spectroscopy”, Optics Comm 9, 319.

    Article  ADS  Google Scholar 

  159. E E Uzgiris and J H Kaplan, 1974, “Study of lymphocyte and erythrocyte electrophoretic mobility by laser Doppler spectroscopy”, Anal Biochem 60, 455.

    Article  Google Scholar 

  160. E E Uzgiris, 1974, “Laser Doppler Spectrometer for study of electrokinetic phenomena”, Rev Sci Instrum 45, 74.

    Article  ADS  Google Scholar 

  161. E E Uzgiris and J H Kaplan, 1974, “Protein coated electrodes”, Rev Sci Instrum 45, 120.

    Article  ADS  Google Scholar 

  162. E E Uzgiris and D C Golibersuch, 1974, “Excess scatteredlight intensity fluctuations from haemoglobin”, Phys Rev Letters 32, 37.

    Article  ADS  Google Scholar 

  163. E E Uzgiris and H P M Fromageot, 1976, “Thickness and density of protein films by optical mixing spectroscopy”, Biopolymers 15, 257.

    Article  Google Scholar 

  164. E E Uzgiris and J H Caplan, 1976, “Laser doppler spectroscopic studies of the electrokinetic properties of human blood cells in dilute salt solutions”, J Colloid Interface Sci 55, 148.

    Article  Google Scholar 

  165. A Wada, 1974, “Rotational relaxation of macromolecules determined by dynamic light scattering. Comments”, Biopolymers 13, 237.

    Article  Google Scholar 

  166. B R Ware, T Raj, W H Flygare, J A Lesnaw and M E Reichmann, 1973, “Molecular weights of vesicular stomatitis virus and its defective particles by laser light scattering spectro-scopy”, J Virol 11, 141.

    Google Scholar 

  167. B R Ware, 1974, “Electrophoretic light scattering”, Advan Colloid Interface Sci 4, 1.

    Article  Google Scholar 

  168. W W Wilson, M R Luzzana, J T Penniston and C S Johnson, 1974, “Pregelation aggregation of sickle cell haemoglobin”, Proc Nat Acad Sci 71, 1260.

    Article  ADS  Google Scholar 

  169. K L Wun, G T Feke and W Prins, 1974, “Laser light scattering by polymer gels”, Faraday Disc Chem Soc 57, 146.

    Article  Google Scholar 

  170. K L Wun and W Prins, 1975, “Histone-induced conformational changes in DNA as probed by quasielastic light scattering”, Biopolymers 14, 111.

    Article  Google Scholar 

  171. I Salmeen, L Rimai, R B Luftig, L Liebes, E Retzel, M Rich and J J McCormick, 1976, “Hydrodynamic diameters of murine mammary, Rous sarcoma and feline leukemia RNA tumor viruses: Studies by laser beat frequency light-scattering spectroscopy and electron microscopy”, J Virology 17, 584.

    Google Scholar 

  172. D W Schaefer and B J Berne, 1974, “Dynamics of charged macromolecules in solution”, Phys Rev Lett 32, 1110.

    Article  ADS  Google Scholar 

  173. D W Schaefer and B J Ackerson, 1975, “Melting of colloidal crystals”, Phys Rev Letters 35, 1448.

    Article  ADS  Google Scholar 

  174. T Schleich and Y Yeh, 1973, “The solution behavior of poly-L-proline: I Light scattering studies in water and concentrated aqueous neutral salt solutions”, Biopolymers 12, 993.

    Article  Google Scholar 

  175. R L Schmidt, 1973, “Observation of the internal motion in N1-DNA”, Biopolymers 12, 1427.

    Article  Google Scholar 

  176. K S Schmitz and J M Schurr, 1973, “Rotational relaxation of macromolecules determined by dynamic light scattering. II Temperature dependence for DNA”, Biopolymers 12 1543.

    Article  Google Scholar 

  177. K S Schmitz, 1974, “Quasielastic light scattering from non-ideal solutions”, Macromolecules 7, 146.

    Article  ADS  Google Scholar 

  178. K S Schmitz and R Pecora, 1975, “Quasi-elastic light scattering by calf thymus DNA and lambda DNA”, Biopolymers 14, 521.

    Article  Google Scholar 

  179. J M Schurr and K S Schmitz, 1973, “Rotational relaxation of macromolecules determined by dynamic light scattering. I Tobacco mosaic virus”, Biopolymers 12, 1021.

    Article  Google Scholar 

  180. D B Seilen, 1973, “Light scattering Rayleigh linewidth measurements on globular protein solutions”, Polymer 14, 359.

    Article  Google Scholar 

  181. D B Seilen, 1975, “Light scattering Rayleigh linewidth measurements on solutions of cellulose trinitrate”, Polymer 16, 169.

    Article  Google Scholar 

  182. D B Seilen, 1975, “Light scattering Rayleigh linewidth measurements on some dextran solutions”, Polymer 16, 561.

    Article  Google Scholar 

  183. D B Seilen, 1975, “Light scattering Rayleigh linewidth measurements upon solutions of globular proteins containing high molecular weight impurities”, Polymer 16, 773.

    Article  Google Scholar 

  184. J C Selser, Y Yeh and R J Baskin, 1976, “A light-scattering characterization of membrane vesicles”, Biophys J 16, 337.

    Article  Google Scholar 

  185. J C Selser and Y Yeh, 1976, “A light scattering method of measuring membrane vesicle number-averaged size and size dispersion”, Biophys J 16, 847.

    Article  Google Scholar 

  186. I W Shepherd, 1975, “Inelastic laser light scattering from synthetic and biological polymers”, Rep Prog Phys 38, 565.

    Article  ADS  Google Scholar 

  187. C M Sorensen, B J Ackerson, R C Mockler and W J O’Sullivan, 1975, “Brownian-diffusion viscosity measurements in a thermal gradient”, Phys Rev Letters 34, 1194.

    Article  ADS  Google Scholar 

  188. C M Sorensen, F R Fickett, R C Mockler, W J O’Sullivan and J F Scott, 1976, “On lysozyme as a possible high-temperature superconductor”, J Phys C (GB) 9, L251.

    Article  ADS  Google Scholar 

  189. S P Spragg, J K Wilcox, J J Roche, and W A Barnett, 1976, “The association of yeast phosphoglycerate kinase”, Biochem J 153, 423.

    Google Scholar 

  190. K L Wun and F D Carlson, 1975, “Harmonically bound particle model for quasielastic light scattered by gels”, Macromolecules 8, 190.

    Article  ADS  Google Scholar 

  191. T Yoshimura, A Kikkawa and N Suzuki, 1975, “Measurements of electrophoretic movements with an optical beating spectrometer”, Jap J Appl Phys 14, 1853.

    Article  ADS  Google Scholar 

  192. T Yoshimura, A Kikkawa and N Suzuki, 1975, “The spectral profile of light scattered by particles in electrophoretic movement”, Optics Commun 15, 277.

    Article  ADS  Google Scholar 

  193. S R Aragon and R Pecora, 1976, “Theory of dynamic light scattering from polydisperse systems”, J Chem Phys 64, 2395.

    Article  ADS  Google Scholar 

  194. W N Huang, E Vrancken and J E Frederick, 1973, “Comparison of the effects of polydispersity on the Rayleigh linewidth as determined by homodyning and heterodyning spectrometry”, Macromolecules 6, 58.

    Article  ADS  Google Scholar 

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Authors and Affiliations

  1. Department of Physics, City College - CUNY, New York, NY, 10031, USA

    H Z Cummins

  2. Royal Signals and Radar Establishment, Malvern, Worcs, WR14 3PS, UK

    P N Pusey

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  1. H Z Cummins
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  1. City College - CUNY, New York, New York, USA

    H. Z. Cummins

  2. Royal Radar and Signals Establishment, Worcestershire, England

    E. R. Pike

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Cummins, H.Z., Pusey, P.N. (1977). Dynamics of Macromolecular Motion. In: Cummins, H.Z., Pike, E.R. (eds) Photon Correlation Spectroscopy and Velocimetry. Nato Advanced Study Institutes Series, vol 23. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-1668-9_5

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