UV-Induced DNA to Protein Cross-Linking in Mammalian Cells

  • Paul Todd
  • Antun Han


Evidence for the cross-linking of DNA to protein was discovered almost simultaneously in UV-irradiated microorganisms and mammalian cells (Smith, 1962; Alexander and Moroson, 1962). Subsequent experiments in which DNA to protein adducts were sought in mixtures of protein and DNA exposed to ultraviolet light clearly indicated that such a reaction is possible (Smith, 1962; 1964a, b; 1967; Sklobovskaya and Ryabchenko, 1970a, b). This report has two aims: first, to summarize the information indicating that DNA-protein cross-links are induced in mammalian cells by ultraviolet light, and second, to review experimental studies that attempt to correlate cross-link induction and biological damage. The demonstration of cross-linking has been done in two ways: (1) the detection of unextractable DNA in irradiated cells, and (2) the isolation and identification of specific photoproducts from irradiated cells. Correlations concerning the involvement of protein in UV-induced lethality have been made utilizing (1) action and absorption spectra, (2) the investigation of biological damage not attributable to pyrimidine dimers, (3) correlations of cross-link induction with biological effect under various experimental conditions, and (4) a search for repair processes associated with DNA-protein cross-linking.


Mammalian Cell Ultraviolet Light Action Spectrum Chinese Hamster Cell Pyrimidine Dimer 
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Copyright information

© Springer Science+Business Media New York 1976

Authors and Affiliations

  • Paul Todd
    • 1
  • Antun Han
    • 2
  1. 1.Department of BiophysicsThe Pennsylvania State UniversityUSA
  2. 2.Division of Biological and Medical ResearchArgonne National LaboratoryArgonneUSA

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