Abstract
There is now substantial evidence which points to the occurrence and biological importance of the formation of protein and other adducts to DNA after ultraviolet or ionizing radiation of cells (Smith, 1975a). Many of the reactive cross-linking agents formed on the irradiation of cells (e.g., free radicals and their derivatives) are thought to exist naturally within a cell but at a much lower concentration. In addition, there exist naturally within the cell many non-radical chemical agents such as the aldehydes which could also cross-link macromolecules (Harman, 1962; Bjorksten, 1962, 1974; Sinex, 1964). The possible parallel between radiation and naturally induced DNA adducts appears sufficiently great to warrant serious consideration of the natural occurrence of DNA adducts as an important primary aging process. The objective of this paper is to review the concept of primary aging processes and the cross-linkage hypothesis of aging and to present the major evidence indicating the natural accumulation of DNA adducts in the chromatin of mammalian species with increasing age.
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References
Acsadi, G., and Nemeskeri, J., 1970, “History of Human Lifespan and Mortality,” Akademiai Kiado, Budapest.
Adelman, R.C., 1972, Age-dependent control of enzyme adaptation, in “Advances in Gerontological Research” Vol. 4 (B.L. Strehler, ed.), pp. 1–23, Academic Press, New York.
Adhikari, H.R., and Tappel, A.L., 1975, Fluorescent products from irradiated amino acids and proteins, Radiat. Res. 61: 177–183.
Ambe, K.S., and Tappel, A.L., 1961, Oxidative damage to amino acids, peptides and proteins by radiation, J. Food Sci. 26: 448–451.
Amici, D., Gianfranceschi, G.L., Marsili, G., and Michetti, L., 1974, Young and old rats. ATP, alkaline phosphatase, cholesterol and protein levels in the blood; DNA and RNA contents of the liver. Regulation by an aqueous thymus extract, Experientia 30: 633–635.
Ansevin, A.T., and Brown, B.W., 1971, Specificity in the association of histones with deoxyribonucleic acid. Evidence from derivative thermal denaturation profiles, Biochemistry 7: 1133–1142.
Ansevin, A.T., Hnilica, L.S., Spelsberg, T.C., and Kehm, S.L., 1971, Structure studies on chromatin and nucleohistones. Thermal denaturation profiles recorded in the presence of urea, Biochemistry 10: 4793–4803.
Ansevin, A.T., Macdonald, K.K., Smith, C.E., and Hnilica, L.S., 1975, Mechanisms of chromatin template activation. Physical evidence for destabilization of nucleoproteins by polyanions, J. Biol. Chem. 250: 281–289.
Bailey, A.J., 1969, The stabilization of the intermolecular cross-links of collagen with ageing, Gerontologia 15: 65–76.
Bailey, A.J., Robins, S.P., and Balian, G., 1974, Biological significance of the intermolecular crosslinks of collagen, Nature 251: 105–109.
Barber, A.A., and Bernheim, F., 1967, Lipid peroxidation, its measurement, occurrence, and significance in animal tissues, in “Advances in Gerontological Research” Vol. 2 (B.L. Strehler, ed.), pp. 355–403, Academic Press, New York.
Bender, A.D., Kormendy, C.G., and Powell, R., 1970, Pharmacological control of aging, Exp. Gerontol. 5: 97–129.
Bender, M.A., Griggs, H.G., and Bedford, J.S., 1974, Mechanisms of chromosomal aberration production III. Chemicals and ionizing radiation, Mutat. Res. 23: 197–212.
Berdyshev, G.D., and Zhelabovskaya, S.M., 1972, Composition, template properties and thermostability of liver chromatin from rats of various age at deproteinization by NaC1 solutions, Exp. Gerontol. 7: 321–330.
Biessmann, H., and Rajewsky, M.F., 1975, Nuclear protein patterns in developing an adult brain and in ethylnitrosourea-induced neuroectodermal tumours of the rat, J. Neurochem. 24: 387–393.
Biology Data Book. Vol. 1, 1972, (P. Altman and D. Dittmer, eds.), p. 229, Fed. Am. Soc. Exp. Biol.
Bjorksten, J., 1962, Aging: present status of our chemical knowledge, J. Am. Geriat. Soc. 10: 125–139.
Bjorksten, J., 1969, Theories, in “Aging Life Processes” (S. Baker-man, ed.), pp. 147–179, C.C. Thomas Pub., Springfield, Illinois.
Bjorksten, J., 1974, Crosslinkage and the aging process, in “Theoretical Aspects of Aging” (M. Rockstein, ed.), pp. 43–59, Academic Press, New York.
Bojanovic, J.J., Jevtovic, A.D., Pantic, V.S., Dugandzic, S.M., and Jovanovic, D.S., 1970, Thymus nucleoproteins. Thymus histones in young and adult rats, Gerontologia 16: 304–312.
Bondy, S.C., and Roberts, S., 1967, Messenger ribonucleic acid of cerebral nuclei, Biochem. J. 105: 1111–1117.
Bondy, S.C., and Roberts, S., 1968, Hybridizable ribonucleic acid of rat brain, Biochem. J. 109: 533–541.
Bondy, S.C., and Roberts, S., 1969, Developmental and regional variations in ribonucleic acid and synthesis on cerebral chromatin, Biochem. J. 115: 341–349.
Bondy, S.C., and Roberts, S., 1970, Histone-acetylating enzyme of brain, Biochem. J. 119: 665–672.
Britten, R., and Davidson, E., 1971, Repetitive and nonrepetitive DNA sequencies and a speculation of the origins of evolutionary novelty, Quart. Rev. Biol. 46: 111–133.
Brooks, A.L., Mead, D.K., and Peters, R.F., 1973, Effect of aging on the frequency of metaphase chromosome aberrations in the liver of the Chinese hamster, J. Gerontol. 28: 452–454.
Burnet, M., 1974, “Intrinsic Mutagenesis: A Genetic Approach to Ageing,” J. Wiley, New York.
Carter, D.B., and Chae, C., 1975, Composition of liver histones in aging rat and mouse, J. GerontoZ. 30: 28–32.
Chio, K.S., and Tappel, A.L., 1969a, Synthesis and characterization of the fluorescent products derived from malonaldehyde and amino acids, Biochemistry 8: 2821–2827.
Chio, K.S., and Tappel, A.L., 1969b, Inactivation of ribonuclease and other enzymes by peroxidizing lipids and by malonaldehyde, Biochemistry 8: 2827–2832.
Comfort, A., 1974, The position of aging studies, Mech. Ageing Develop. 3: 1–31.
Comings, D.E., and Vance, C.K., 1971, Thermal denaturation of DNA and chromatin of early and late passage human fibroblasts, Gerontologia 17: 116–121.
Courtois, Y.G.C., 1974, Chromatin modification of chick embryo cells during in vitro senescence, Mech. Ageing Develop. 3: 51–63.
Crowley, C., and Curtis, H.J., 1963, The development of somatic mutations in mice with age, Proc. Nat. Acad. Sci. USA 49: 626–628.
Curtis, H.J., Leith, J., and Tilley, J., 1966, Chromosome aberrations in liver cells of dogs of different ages, J. GerontoZ. 21: 268–270.
Curtis, H.J., and Miller, K., 1971, Chromosome aberrations in liver cells of guinea pigs, J. Gerontol. 26: 292–293.
Cutler, R.G., 1972, Transcription of reiterated DNA sequence classes throughout the lifespan of the mouse, in “Advances in Gerontological Research” Vol. 4 (B.L. Strehler, ed.), pp. 219–321, Academic Press, New York.
Cutler, R.G., 1974, Redundancy of information content in the genome of mammalian species as a protective mechanism determining aging rate, Mech. Ageing Develop. 2: 381–408.
Cutler, R.G., 1975a, Evolution of human longevity and the genetic complexity governing aging rate, Proc. Nat. Acad. Sci. USA (in press).
Cutler, R.G., 1975b, Evolution of longevity in mammals. I. Primates, J. Human Evol. (in press).
Cutler, R.G., 1975c, Evolution of longevity in mammals. II. Ungulates and carnivores, J. Human Evol. (in press).
Cutler, R.G., 1975d, Transcription of unique and reiterated DNA sequences in mouse liver and brain tissues as a function of age, Exp. Gerontol. 10: 37–60.
David, J., Gordon, J.S., and Rutter, W.J., 1974, Increased thermal stability of chromatin containing 5-bromodeoxyuridine-substituted DNA, Proc. Nat. Acad. Sci. USA 71: 2808–2812.
Demopoulos, H.B., 1973a, The basis of free radical pathology, Fed. Proc. 32: 1859–1861.
Demopoulos, H.B., 1973b, Control of free radicals in biological systems, Fed. Proc. 32: 1903–1908.
Deyl, Z., Juricova, M., Rosmus, J., and Adam, M., 1971, Aging of the connective tissue: collage cross linking in animals of different species and equal ages, Exp. Gerontol. 6: 227–233.
Luzio, N.R., 1973, Antioxidants, lipid peroxidation and chemical-induced liver injury, Fed. Proc. 32: 1875–1881.
Dormandy, T.L., 1969, Biological rancidification, Lancet, Sept. 27: 684–688.
Ebbesen, P., 1974, Aging increases susceptibility of mouse skin to DMBA carcinogenesis independent of general immune status, Science 183: 217–218.
Eigner, J., Boedtker, H., and Michaels, G., 1961, The thermal degradation of nucleic acids, Biochim. Biophys. Acta 51: 165–168.
Epstein, J., and Gershon, D., 1972, Studies on ageing in nematodes. IV. The effect of antioxidants on cellular damage and life span, Mech. Ageing Develop. 1: 257–264.
Epstein, J., Himmelhock, S., and Gershon, D., 1972, Studies on ageing in nematodes. III. Electronmicroscopical studies on age-associated cellular damage, Mech. Ageing Develop. 1: 245–255.
Epstein, J., Williams, J.R., and Little, J.B., 1973, Deficient DNA repair in human progeroid cells, Proc. Nat. Acad. Sci. USA 70: 977–981.
Finch, C.E., 1969, “Cellular Activities During Ageing in Mammals,” MSS Inform. Corp., New York.
Goldblatt, H., and Friedman, L., 1974, Prevention of malignant change in mammalian cells during prolonged culture in vitro, Proc. Nat. Acad. Sci. USA 71: 1780–1782.
Goldstein, S., and Moerman, E.J., 1975, Defective proteins in normal and abnormal human fibroblasts during aging in vitro, Gerontologia (in press).
Goldstein, S., Niewiarowski, S., and Singal, D.P., 1975, Pathological implications of cell aging in vitro, Fed. Proc. 34: 56–63.
Gordon, P., 1974, Free radicals and the aging process, in “Theoretical Aspects of Aging” (M. Rockstein, ed.), pp. 61–81, Academic Press, New York.
Green, J., 1972, Vitamin E and the biological antioxidant theory, Annu. New York Acad. Sci. 203: 29–44.
Grouse, L., Chilton, M., and McCarthy, B.J., 1972, Hybridization of RNA with unique sequences of mouse DNA, Biochemistry 11: 798–805.
Habazin, V., and Han, A., 1970, Ultra-violet-light-induced DNA-toprotein cross-linking in Hela cells, Int. J. Radiat. Biol. 17: 569–575.
Hagen, U., Ullrich, M., and Jung, H., 1969, Transcription on irradiated DNA, Int. J. Radiat. Biol. 16: 597–601.
Hagen, U., Ullrich, M., Petersen, E.E., Werner, E., and Kroger, H., 1970, Enzymatic RNA synthesis on irradiated DNA, Biochim. Biophys. Acta 199: 115–125.
Hahn, H.P. von, 1963, Age-dependent thermal denaturation and viscosity of crude and purified DNA prepared from bovine thymus, Gerontologia 8: 123–131.
Hahn, H.P. von, 1964, Age-related alterations in the structure of DNA. II. The role of histones, Gerontologia 10: 174–182.
Hahn, H.P. von, 1970a, The regulation of protein synthesis in the ageing cell, Exp. Gerontol. 5: 323–334.
Hahn, H.P. von, 1970b, Structural and functional changes in nucleoprotein during the ageing of the cell, Gerontologia 16: 116–128.
Hahn, H.P. von, and Fritz, E., 1966, Age-related alterations in the structure of DNA. III. Thermal stability of rat liver DNA, related to age, histone content and ionic strength, Gerontologia 12: 237–250.
Hahn, H.P. von, Miller, J., and Eichhorn, G.L., 1969, Age-related alterations in the structure of nucleoprotein. IV. Changes in the composition of whole histone from rat liver, GerontoZogia 15: 293–301.
Hall, D.A., 1968, The ageing of connective tissue, Exp. Gerontol. 3: 77–89.
Hall, D.A., 1969, Connective tissues, in “Aging Life Processes” (S. Bakerman, ed.), pp. 79–122, C.C. Thomas, Pub., Springfield, Ill.
Han, A., Korbelik, M., and Ban, J., 1975, DNA-to-protein cross-linking in synchronized Hela cells exposed to ultra-violet light, Int. J. Radiat. Biol. 27: 63–74.
Harman, D., 1956, Aging: a theory based on free radical and radiation chemistry, J. Gerontol. 11: 298–300.
Harman, D., 1961, Prolongation of the normal lifespan and inhibition of spontaneous cancer by antioxidants, J. Gerontol. 16: 247–254.
Harman, D., 1962, Role of free radicals in mutation, cancer, aging and the maintenance of life, Radiat. Res. 16: 753–763.
Harman, D., 1967, Chromatin template capacity: effect of oxygen, abstract, The Gerontologist: Part II, 7: 2–9.
Harman, D., 1968, Free radical theory of aging: effect of free radical reaction inhibitors on the mortality rate of male LAF1 mice, J. GerontoZ. 23: 476–482.
Harman, D., 1969, Prolongation of life: role of free radical reactions in aging, J. Am. Geriat. Soc. 17: 721–734.
Harman, D., 1971, Free radical theory of aging: effect of the amount and degree of unsaturation of dietary fat on mortality rate, J. Gerontol. 26: 451–457.
Harman, D., 1972a, Free radical theory of aging: dietary implications, Am. J. Clin. Nutri. 25: 839–843.
Harman, D., 1972b, Increasing the healthy life span, Acta Geront. BeZg. 10: 83–88.
Harman, D., Curtis, H.J., and Tilley, J., 1970, Chromosomal aberrations in liver cells of mice fed free radical reaction inhibitors, J. Gerontol. 25: 17–19.
Hart, R.W., and Setlow, R.B., 1974, Correlation between deoxyribonucleic acid excision repair and lifespan in a number of mammalian species, Proc. Nat. Acad. Sci. USA 71: 2169–2173.
Hart, R.W., and Trosko, J.E., 1975, DNA repair processes in mammals, Gerontologia (in press).
Hayflick, L., 1974, Cytogerontology, in “Theoretical Aspects of Aging” (M. Rockstein, ed.), pp. 83–103, Academic Press, New York.
Hjelm, R.P., and Huang, R.C.C., 1974, The role of histones in the conformation of DNA in chromatin as studied by circular dichroism, Biochemistry 13: 5275–5283.
Hoffer, A., and Roy, R.M., 1975, Vitamin E decreases erythrocyte fragility after whole-body irradiation, Radiat. Res. 61: 439–443.
Holliday, R., and Pugh, J.E., 1975, DNA modification mechanisms and gene activity during development, Science 187: 226–232.
Ivannik, B.P., and Ryabchenko, N.I., 1969, Some physicochemical changes in DNA isolated from the organs of irradiated rats, Radiobiologiya 9: 7–14.
Jackson, D.S., 1973, Some properties of the cross-linking theory of ageing of collagen, in “Connective Tissue and Aging” (H.G. Vogel, ed.), pp. 191–194, Excerpta Medica, Amsterdam.
Jones, M.L., 1968, Longevity of primates in captivity, International Zoo Yearbook 8: 183–192.
Kaldor, G., and Min, B.K., 1975, Enzymatic studies on the skeletal myosin A and actomyosin of aging rats, Fed. Proc. 34: 191–194.
Kawashima, S., 1970, The possible role of lipoperoxide in aging, Nagoya J. Med. Sci. 32: 303–326.
King, M., and Wilson, A.C., 1975, Evolution at two levels in humans and chimpanzees, Science 188: 107–116.
Kirby, K.S., 1958, Preparation of some deoxyribonucleic acid-protein complexes from rat-liver homogenates, Biochem. J. 70: 260–265.
Kirby, K.S., 1967, Isolation of deoxyribonucleic acid from mammalian tissues, Biochem. J. 104: 254–257.
Kohn, R.R., 1963, Human aging and disease, J. Chron. Dis. 16: 5–21.
Kohn, R.R., 1971a, “Principles of Mammalian Aging,” Prentice-Hall, Englewood Cliffs, New Jersey.
Kohn, R.R., 1971b, Effect of antioxidants on life-span of C57BL mice, J. GerontoZ. 26: 378–380.
Kurtz, D.I., Russell, A.P., and Sinex, F.M., 1974, Multiple peaks in the derivative melting curve of chromatin from animals of varying age, Mech. Ageing Develop. 3: 37–49.
Kurtz, D.I., and Sinex, F.M., 1967, Age related differences in the association of brain DNA and nuclear protein, Biochim. Biophys. Acta 145: 840–842.
Leveson, J.E., and Peacocke, A.R., 1966, Studies of a complex of deoxyribonucleic acid with non-histone protein, Biochim. Biophys. Acta 123: 329–336.
Li, H.J., and Bonner, J., 1971, Interactions of histone half-molecules with deoxyribonucleic acid, Biochemistry 10: 1461–1470.
Li, H.J., Chang, C., and Weiskopf, M., 1973, Helix-coil transition in nucleoprotein-chromatin structure, Biochemistry 12: 1763–1772.
Liew, C.C., and Gornall, A.G., 1975, Covalent modification of nuclear proteins during aging, Fed. Proc. 34: 186–187.
Lloyd, P.H., Nicholson, B.H., and Peacocke, A.R., 1967, The effects of y-irradiation on the priming by deoxyribonucleohistone of ribonucleic acid polymerase, Biochem. J. 104: 999–1003.
Lucy, J.A., 1972, Functional and structural aspects of biological membranes: a suggested structural role for vitamin E in the control of membrane permeability and stability, Annu. New York Acad. Sci. 203: 4–11.
Massie, H.R., Baird, M.B., Nicolosi, R.J., and Samis, H.V., 1972, Changes in the structure of rat liver DNA in relation to age, Arch. Biochem. Biophys. 153: 736–741.
Maynard Smith, J., 1966, Theories of aging, in “Topics in the Biology of Aging,” (P.L. Krohn, ed.), pp. 1–27, J. Wiley and Sons, New York.
McKusick, V.A., 1971, “Mendelian Inheritance in Man,” Johns Hopkins Press, Baltimore, Maryland.
Milvy, P., 1973, Control of free radical mechanisms in nucleic acid systems: studies in radioprotection and radiosensitivity, Fed. Proc. 32: 1895–1902.
Miner, R.W., 1954, “Parental age and characteristics of the offspring, Annu. New York Acad. Sci. 57: 451–462.
Modak, S.P., and Price, G.B., 1971, Exogenous DNA polymerase-catalyzed incorporation of deoxyribonucleotide monophosphates in nuclei of fixed mouse brain cells. Changes associated with age and x-irradiation, Exp. Cell Res. 65: 289–296.
Nair, P.P., and Kayden, H.J., eds., 1972, “Vitamin E and Its Role in Cellular Metabolism,” Annu. New York Acad. Sci., Vol. 23.
Ohno, S., 1970, “Evolution by Gene Duplication,” Springer Verlag, Heidelberg.
O’Meara, A.R., and Herrmann, R.L., 1972, A modified mouse liver chromatin preparation displaying age-related differences in salt dissociation and template ability, Biochim. Biophys. Acta 269: 419–427.
Packer, L., Deamer, D.W., and Heath, R.L., 1967, Regulation and deterioration of structure in membranes, in “Advances in Gerontological Research” (B.L. Strehler, ed.), pp. 77–120, Academic Press, New York.
Packer, L., and Smith, J.R., 1974, Extension of the lifespan of cultured normal human diploid cells by vitamin E, Proc. Nat. Acad. Sci. USA 71: 4763–4767.
Panyim, S., and Chalkley, R., 1969, High resolution acrylamide gel electrophoresis of histones, Arch. Biochem. Biophys. 130: 337–346.
Penrose, L.S., 1954, Mongolism idiocy (mongolism) and maternal age, Annu. New York Acad. Sci. 57: 494–502.
Petes, T.D., Farber, R.A., Tarrant, G.M., and Holliday, R., 1974, Altered role of DNA replication in ageing human fibroblast cultures, Nature 251: 434–436.
Price, G.B., and Makinodan, T., 1973, Aging: alteration of DNA-protein information, Gerontologia 19: 58–70.
Price, G.B., Modak, S.P., and Makinodan, T., 1971, Age-associated changes in the DNA of mouse tissue, Science 171: 917–920.
Pryor, W.A., 1970, Free radicals in biological systems, Scien. Amer. 223: 70–83.
Pryor, W.A., 1971, Free radical pathology, Chem. Eng. News 49: 34–51.
Pryor, W.A., 1973, Free radical reactions and their importance in biochemical systems, Fed. Proc. 32: 1862–1869.
Pyhtila, M.J., and Sherman, F.G., 1968a, Age-associated studies on thermal stability and template effectiveness of DNA and nucleoproteins from beef thymus, Biochem. Biophys. Res. Commun. 31: 340–344.
Pyhtila, M.J., and Sherman, F.G., 1968b, Studies on the histones and DNA of calf and old cow thymus, J. Gerontol. 23: 450–453.
Riely, C.A., Cohen, G., and Lieberman, M., 1974, Ethane evolution: A new index of lipid peroxidation, Science 183: 208–210.
Roberts, S., 1973, Alterations in cerebral protein. Synthesizing systems during maturation, in “Neurobiological Aspects of Maturation and Aging,” (D.H. Ford, ed.), pp. 277–292, Elsevier Pub. Co., New York.
Ross, M., 1961, Length of life and nutrition in the rat, J. Nutrit. 75: 197–210.
Roth, G.S., 1974, Age-related changes in specific glucocorticoid binding by steroid-responsive tissues of rats, Endocrinology 94: 82–90.
Roth, G.S., 1975, Age-related changes in glucocorticoid binding by rat splenic leukocytes: possible cause of altered adaptive responsiveness, Fed. Proc. 34: 183–185.
Roth, G.S., and Adelman, R.C., 1973, Possible changes in tissue sensitivity in the age-dependent stimulation of DNA synthesis in vivo, J. Gerontol. 28: 298–301.
Roti, J.L., Stein, G.S., and Cerutti, P.A., 1974, Reactivity of thymine to x-rays in Hela chromatin and nucleoprotein preparations, Biochemistry 13: 1900–1905.
Russell, A.P., Dowling, L.E., and Herrmann, R.L., 1970, Age-related differences in mouse liver DNA melting and hydroxylapatite fractionation, Gerontologia 16: 159–171.
Ryan, J.M., and Cristofalo, V.J., 1972, Histone acetylation during aging of human cells in culture, Biochem. Biophys. Res. Commun. 48: 735–742.
Sacher, G., 1959, Relation of lifespan to brain weight and body weight in mammals, in “Ciba Found. Colloquium on Aging,” Vol. 5, pp. 115–141, Churchill, London.
Sacher, G., 1975, Maturation and longevity in relation to cranial capacity in hominid evolution, in “Antecedents of Man and After. Vol 1, Primates: Functional Morphology and Evolution,” Mouton, The Hague (in press).
Salser, J.S., and Balis, M.E., 1967, Investigations on amino acids bound to DNA, Biochim. Biophys. Acta 149: 220–227.
Salser, J.S., and Balis, M.E., 1968, Amino acids associated with DNA of tumors, Cancer Res. 28: 595–600.
Salser, J.S., and Balis, M.E., 1969, The effect of growth conditions on amino acids bound to deoxyribonucleic acid, J. Biol. Chem. 244: 822–828.
Salser, J.S., and Balis, M.E., 1972, Alterations in deoxyribonucleic acid-bound amino acids with age and sex, J. GerontoZ. 27: 1–9.
Samis, H.V., Poccia, D.L., and Wulf, V.J., 1968, The effect of salt extraction and heat-denaturation on the behavior of rat liver chromatin, Biochim. Biophys. Acta 166: 410–418.
Samis, H.V., and Wulf, V.J., 1969, The template activity of rat liver chromatin, Exp. GerontoZ. 4: 111–117.
Schott, H.N., and Shetlar, M.D., 1974, Photochemical addition of amino acids to thymine, Biochem. Biophys. Res. Commun. 59: 1112–1116.
Sheldrake, A.R., 1974, The ageing, growth and death of cells, Nature 250: 381–385.
Shirey, T.L., and Sobel, H., 1972, Compositional and transcriptional properties of chromatins isolated from cardiac muscles of young-mature and old dogs, Exp. GerontoZ. 7: 15–29.
Sinex, F.M., 1964, Cross-linkage and ageing, in “Advances in Gerontological Research,” Vol. 1 (B.L. Strehler, ed.), pp. 165–180, Academic Press, New York.
Sinex, F.M., 1974, The mutation theory of aging, in “Theoretical Aspects of Aging” (M. Rockstein, ed.), pp. 23–31, Academic Press, New York.
Sklobovskaya, M.V., and Ryabchenko, N.N., 1970, Reduction of the yield of DNA in the deproteinization of UV or gamma-irradiated solutions of deoxyribonucleoprotein I. Dependence of the magnitude of the effect on the completeness of the complexation of DNA with protein, RadiobioZogiya 10: 18–24.
Slater, T.F., 1972, “Free Radical Mechanisms in Tissue Culture,” Pion Ltd., London.
Smets, L.A., and Cornelis, J.J., 1971, Repairable and irrepairable damage in 5-bromouracil-substituted DNA exposed to ultraviolet radiation, Int. J. Radiat. BioZ. 19: 445–457.
Smith, K.C., 1975a, The radiation-induced addition of proteins and other molecules to nucleic acids, in “Photochemistry and Photobiology of Nucleic Acids,” (S.Y. Wang, ed.), pp. Academic Press, New York.
Smith, K.C., 1975b, Chemical adducts to DNA: their importance to the genetic alteration theory of aging, Gerontologia (in press).
Stanley, J.F., Pye, D., and MacGregor, A., 1975, Comparison of doubling numbers attained by cultured animal cells with life spans of species, Nature 255: 158–159.
Stein, G.S., and Stein, J.L., 1975, In vitro studies of transcription as a function of age in mammalian cells, Gerontologia (in press).
Stein, G.S., Wang, P.L., and Adelman, R.C., 1973, Age-dependent changes in the structure and function of mammalian chromatin. I. Variations in chromatin template activity, Exp. GerontoZ. 8: 123–133.
Strehler, B.L., ed., 1960, “The Biology of Aging,” A.I.B.S. Pub. No. 6, p. 23, Waverly Press, Baltimore.
Strehler, B.L., 1962, “Time, Cells, and Aging,” Academic Press, New York.
Strehler, B.L., 1964, On the histochemistry and ultrastructure of age pigment, in “Advances in Gerontological Research,” Vol. 1 (B.L. Strehler, ed.), pp. 343–384, Academic Press, New York.
Strehler, B.L., 1975, Implications of aging research for society, Fed. Proc. 34: 5–8.
Takacs, I., and Verzar, F., 1968, Macromolecular aging of collagen. I. Experiments in vivo and in vitro with different animal races, Gerontologia 14: 15–23.
Tanzer, M.L., 1973, Cross-linking of collagen, Science 180: 561–566.
Tappel, A.L., 1965, Free-radical lipid peroxidation damage and its inhibition by vitamin E and selenium, Fed. Proc. 24: 73–78.
Tappel, A.L., 1968, Will antioxidant nutrients slow aging processes? Geriatrics 23: 97–105.
Tappel, A.L., 1972, Vitamin E and free radical peroxidation of lipids, Annu. New York Acad. Sci. 203: 12–28.
Tappel, A.L., 1973, Lipid peroxidation damage to cell components, Fed. Proc. 32: 1870–1874.
Tappel, A.L., Fletcher, B., and Deamer, D., 1973, Effect of antioxidants and nutrients on lipid peroxidation fluorescent products and aging parameters in the mouse, J. Gerontol. 28: 415–424.
Timiras, P.S., ed., 1972, “Developmental Physiology and Aging,” Macmillan Co., New York.
Trosko, J.E., and Hart, R.W., 1975, DNA mutation frequencies in mammals, Gerontologia (in press).
Umansky, S.R., Korol, B.A., Runova, Y.N., and Tokarskaya, V.I., 1974, Possible mechanisms for radiation disturbance of transcription, Int. J. Radiat. Biol. 25: 31–41.
Vanyushin, B.F., Nemerovsky, L.E., Klimenko, V.V., Vasiliev, V.K., and Belozersky, A.N., 1973, The 5-methylcytosine in DNA of rats. Tissue and age specificity and the changes induced by hydrocortisone and other agents, Gerontologia 19: 138–152.
Vernadakis, A., 1975, Neuronal-glia interactions during development and aging, Fed. Proc. 34: 89–95.
Verzar, F., 1963, The aging of collagen, Scien. Amer. 208:104–114. Vogel, H.G., ed., 1973, “Connective Tissue and Aging,” Excerpta Medica, Amsterdam.
Weiss, J.J., and Wheeler, C.M., 1964, Effect of y-radiation on deoxynucleoprotein acting as a primer in RNA synthesis, Nature 203: 291–292.
Wheeler, K.T., and Lett, J.T., 1974, On the possibility that DNA repair is related to age in non-dividing cells, Proc. Nat. Acad. Sci. USA 71: 1862–1865.
Wilhelm, F.X., de Murcia, G.M., Champagne, M.H., and Daune, M.P., 1974, Conformational changes of histones and DNA during the thermal denaturation of nucleoprotein, Eur. J. Biochem. 45: 431–443.
Wolpert, L., and Lewis, J.H., 1975, Towards a theory of development, Fed. Proc. 34: 14–20.
Yamanaka, N., and Deamer, D., 1974, Superoxide dismutase activity in WI-38 cell cultures: Effects of age, trypsinization and SV-40 transformation, PhysioZ. Chem. Physics 6: 95–106.
Yielding, K.L., 1974, A model for aging based on differential repair of somatic mutational damage, Persp. BioZ. Med. 17: 201–208.
Yoshii, G., Juwabara, M., and Hayashi, M., 1974, Irradiation effects on deoxyribonucleoprotein in the presence of Ca ion, abstr. A-3–3, Radiat. Res. 59: 7.
Zeman, W., 1971, The neuronal ceroid-lipofuscinoses-Batten-Vogt syndrome: A model for human aging? in “Advances in Gerontological Research,” (B.L. Strehler, ed.), pp. 147–170, Academic Press, New York.
Zhelabovskaya, S.M., and Berdyshev, G.D., 1972, Composition, template activity and thermostability of the liver chromatin in rats of various ages, Exp. Gerontol. 7: 313–320.
Zimmerman, E., Pathak, M.A., and Kornhauser, A., 1972, The in vivo effect of UV irradiation on epidermal chromatin, abstr., Clin. Res. 20: 420.
Zimmerman, F., Kroger, H., Hagen, U., and Kech, K., 1964, The effect of x-irradiation on the priming ability of DNA in the RNA polymerase system, Biochim. Biophys. Acta 87: 160–162.
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Cutler, R.G. (1976). Cross-Linkage Hypothesis of Aging: DNA Adducts in Chromatin as a Primary Aging Process. In: Smith, K.C. (eds) Aging, Carcinogenesis, and Radiation Biology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-1662-7_21
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