Human Brain Tumor Cells in Matrix Culture

  • Teddy Holmström


Organ cultures of various embryonic tissues and of both benign and malignant tumors have been successfully used in various investigations. The basic aim of this technique is to maintain the architecture, differentiation, and growth of the tissue as similar as possible to the in vivo situation. One of the best-known methods is the Trowell-type organ culture system, in which tissue explants are grown on small pieces of lens paper placed on a grid of steel mesh (Trowell, 1954). Although this type of organ culture system allows growth of the fragment as a whole, it does not permit outgrowth of the cells at the periphery of the primary explant. This weakness is partly overcome in three-dimensional matrix culture systems, which permit both maintenance of primary explants and replication of cells with reproduction of original tissue structures within the pores of the supporting matrix.


Nerve Growth Factor Human Brain Tumor Acta Pathol Primary Explants Organ Culture System 
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Copyright information

© Springer Science+Business Media New York 1975

Authors and Affiliations

  • Teddy Holmström
    • 1
  1. 1.Department of PathologyUniversity of HelsinkiHelsinkiFinland

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