Abstract
The ever-increasing availability of human tumor lines in continuous culture poses questions of pertinence to those interested in chromosomes of cancer cells as well as to those interested in the biology of cancer in a more general way. A cursory glance at reports detailing the establishment in vitro of a tumor line reveals that many include karyotype analysis. A simple but central question is, therefore, Is knowledge of the chromosome constitution of the cultured cells useful? That question can be answered intuitively with a yes, or otherwise, it could be said, chromosomal analysis would not be so frequently utilized as an ancillary descriptive method. However, it is possible that cytogeneticists are rather like squirrels who instinctively collect and store away acorns, sometimes for others eventually to retrieve and to make use of ineffectually if at all. Alternatively, the objectives of chromosome analysis of tumor cell cultures may be quite clear in the mind of the initiator. Whichever is the prevailing approach, it appears worthwhile to elaborate and to consider some possible objectives of chromosome studies of human tumor cells in vitro.
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References
American Type Culture Collection, 1972, Registry of Animal Cell Lines (J. E. Shannon and M. L. Macy, ), Rockville, Md.
Atkin, N. B., 1974, Chromosomes in human malignant tumors: A review and assessment, in: Chromosomes and Cancer (J. German, ed.), pp. 375–422, Wiley, New York.
Auersperg, N., 1964, Long-term cultivation of hypodiploid human tumor cells, J. Natl. Cancer Inst. 32: 135.
Auersperg, N., 1966, Karyotype changes of near hexaploid carcinoma cells during adaptation in culture, Nature (Lond.) 212: 635.
Auersperg, N., and Hawryluk, A. P., 1962, Chromosome observations on three epithelial-cell cultures derived from carcinomas of the human cervix, J. Natl. Cancer Inst. 28: 605
Bassin, R. H., Plata, E. J., Gerwin, B. I., Mattem, C. F., Haapala, D. K., and Chu, E. W., 1972, Isolation of a continuous epithelioid cell line, HBT-3, from a human breast carcinoma, Proc. Soc. Exp. Biol. Med. 141: 673.
Berman, L., and Stulberg, C. S., 1956, Eight culture strains (Detroit) of human epithelial-like cells, Proc. Soc. Exp. Biol. Med. 92: 730.
Berman, L., Stulberg, C. S., and Ruddle, F. H., 1957, Human cell culture: Morphology of the Detroit strains, Cancer Res. 17: 668.
Biedler, J. L., Helson, L., and Suciu—Foca, N., 1973a, Expression of HL-A antigens on human neuroblastoma cells in continuous culture, In Vitro 8: 410.
Biedler, J. L., Helson, L., and Spengler, B. A., 1973b, Morphology and growth, tumorigenicity, and cyto genetics of human neuroblastoma cells in continuous culture, Cancer Res. 33: 2643.
Bottomley, R. H., Trainer, A. L., and Griffin, M. J., 1969, Enzymatic and chromosomal characterization of HeLa variants, J. Cell Biol. 41: 806.
Cailleau, R., 1960, The establishment of a cell strain (MAC-21) from a mucoid adenocarcinoma of the human lung, Cancer Res. 20: 837.
Chang, R. S. M., 1954, Continuous subcultivation of epithelial-like cells from normal human tissue, Proc. Soc. Exp. Biol. Med. 87: 440.
Chen, J., Tang, S., Chu, T., and Shen, T., 1962, Preliminary report on the establishment of a strain of human liver cancer cells in vitro, in: VIII International Cancer Congress, p. 136, Moscow.
Chen, T. R., and Shaw, M. W., 1973, Stable chromosome changes in a human malignant melanoma, Cancer Res. 33: 2042.
Chicago Conference: Standardization in Human Cytogenetics, 1966, Birth Defects: Original Article Series,Vol. II, No. 2, The National Foundation, New York.
Chu, E. H. Y., and Giles, N. H., 1958, Comparative chromosomal studies on mammalian cells in culture. I. The HeLa strain and its mutant clonal derivatives, J. Natl. Cancer Inst. 20: 383.
Chu, E. H. Y., and Giles, N. H., 1959, Human chromosome complements in normal somatic cells in culture, Am. J. Hum. Genet. 11: 63.
Cox, D., Yuncken, C., and Spriggs, A. I., 1965, Minute chromatin bodies in malignant tumours of childhood, Lancet 2: 55.
Dobrynin, Y. V., 1963, Establishment and characteristics of cell strains from some epithelial tumors of human origin, J. Natl. Cancer Inst. 31: 1173.
Duran-Troise, G., and DeLustig, E. S., 1972, Cytogenetic studies on a cell line from a mixed parotid tumour, Rev. Eur. Etudes Clin. Biol. 17: 605.
Eagle, H., 1955, Propagation in a fluid medium of a human epidermoid carcinoma strain KB, Proc. Soc. Exp. Biol. Med. 89: 362.
Fjelde, A., 1955, Human tumor cells in tissue culture, Cancer 8: 845.
Fleischmann, T., Hakansson, C. H., and Levan, A., 1971, Fluorescent marker chromosomes in malignant lymphomas, Hereditas 69: 311.
Fleischmann, T., Hakansson, C. H., Levan, A., and Möller, T. 1972, Multiple chromosome aberrations in a lymphosarcomatous tumor., Hereditas 70: 243.
Fogh, J., and Lund, R. 0., 1957, Continuous cultivation of epithelial cell strains (FL) from human amniotic membrane, Proc. Soc. Exp. Biol. Med. 94: 533.
Fogh, J., Ramos, L., and Fogh, H., 1969, Transformation of primary human amnion cells with simian virus 40, in: Axenic Mammalian Cell Reactions ( G. L. Tritsch, ed.), pp. 59–116, Dekker, New York.
Ford, C. E., and Hamerton, J. L., 1956, The chromosomes of man, Nature (Lond.) 178:1020. Fraccaro, M., Lindsten, J., Mannini, A., Scappaticci, S., and Tiepolo, L., 1968, Stability of abnormal karyotypes in cell culture, Hereditas 62: 105.
Fraley, E. E., Ecker, S., and Vincent, M. M., 1970, Spontaneous in vitro neoplastic transformation of adult human prostatic epithelium, Science 170: 540.
Freedman, L. S., Roffman, M., Lele, K. P., Goldstein, M., Biedler, J. L., Spengler, B. A., and Helson, L., 1973, Further studies on dopamine-ß-hydroxylase in neuroblastoma, Fed. Proc. 32: 708.
Frisch, A. W., Jentoft, V., Barger, R., and Losli, E. J., 1955, A human epithelium-like cell (Maben) derived from an adenocarcinoma of lung, Am. J. Clin. Pathol. 25: 1107.
Gaffney, E. W., Fogh, J., Ramos, L., Loveless, J. D., Fogh, H., and Dowling, A. M., 1970
Established lines of SV40-transformed human amnion cells, Cancer Res. 30:1668.
Gartler, S. M., 1967, Genetic markers as tracers in cell culture, Natl. Cancer Inst. Monogr. 26: 167.
Gartler, S. M., 1968, Apparent HeLa cell contamination of human heteroploid cell lines, Nature (Lond.) 217: 750.
Gey, G. O., Coffman, W. D., and Kubicek, M. T., 1952, Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium, Cancer Res 12: 264.
Girardi, A. J. Warren, L., Goldman, C., and Jeffries, B., 1958, Growth and CF antigenicity of measles virus in cells deriving from human heart, Proc. Soc. Exp. Biol. Med. 98:18.
Girardi, A. J., Jensen, F. C., and Koprowski, H., 1965, SV40-induced transformation of human diploid cells: Crisis and recovery, J. Cell. Comp. Physiol. 65: 69.
Hamerton, J. L., 1971, Chromosomes and neoplastic disease, in: Human Cytogenetics,Vol. 2, pp. 407–441, Academic Press, New York and London.
Harnden, D. G., 1974, Viruses, chromosomes and tumours: The interaction between viruses and chromosomes, in: Chromosomes and Cancer (J. German, ed.), pp. 151–190, Wiley, New York.
Hayflick, L., 1961, The establishment of a line (WISH) of human amnion cells in continuous cultivation, Exp. Cell Res. 23:14.
Hayflick, L., 1965, The limited in vitro lifetime of human diploid cell strains, Exp. Cell Res. 37: 614.
Hayflick, L., and Moorhead, P. S., 1961, The serial cultivation of human diploid cell strains, Exp. Cell Res. 25: 585.
Hirose, M., 1968, Tissue culture of human thyroid cancer, Acta Med. Okayama 22:185. Hirschhorn, K., 1973, Chromosome identification, Ann. Rev. Med. 24: 67.
Hsu, T. C., 1954a, Cytological studies on HeLa, a strain of human cervical carcinoma. I. Observations on mitosis and chromosomes, Texas Rep. Biol. Med. 12: 833.
Hsu, T. C., 19546, Mammalian chromosomes in vitro. IV. Some human neoplasms, J. Natl. Cancer Inst. 14: 905.
Hsu, T. C., 1961, Chromosomal evolution in cell populations, Int. Rev. Cytol. 12: 69.
Hsu, T. C., and Moorhead, P. S., 1957, Mammalian chromosomes in vitro. VII. Heteroploidy in human cell strains, J. Natl. Cancer Inst. 18: 463.
Hsu, T. C., Pomerat, C. M., and Moorhead, P. S., 1957, Mammalian chromosomes in vitro. VIII.
Heteroploid transformation in the human cell strain Mayes, J. Natl. Cancer Inst. 19:867.
Ishihara, T., Moore, G. E., and Sandberg, A., 1962, The in vitro chromosome constitution of cells from human tumors, Cancer Res. 22: 375.
Ishii, S., and Nishimura, N., 1966, Establishment and characteristics of a tissue culture strain from metastatic clear-cell carcinoma in human bone, J. Jap. Orthop. Ass. 40: 285.
Jensen, F., Koprowski, H., and Pontén, J., 1963, Rapid transformation of human fibroblast cultures by simian virus 40, Proc. Natl. Acad. Sci 50: 343.
Jones, G. W., Simkovic, D., Biedler, J. L., and Southam, C. M., 1967, Human anaplastic thyroid carcinoma in tissue culture, Proc. Soc. Exp. Biol. Med. 126: 426.
Koller, P. C., 1972, The role of chromosomes in cancer biology, Rec. Results Cancer Res. 38:1.
Kondo, T., Muragishi, H., and Imaizumi, M., 1971, A cell line from a human salivary gland mixed tumor, Cancer 27: 403.
Koprowski, H., Pontén, J. A., Jensen, F., Ravdin, R. G., Moorhead, P. S., and Saksela, E., 1962, Transformation of cultures of human tissue injected with simian virus SV40, J. Cell. Comp. Physiol. 59:281.
Kotler, S., and Lubs, H. A., 1967, Comparison of direct and short-term tissue culture technics in determining solid tumor karyotypes, Cancer Res. 27:1861.
Kuramoto, H., Tamura, S., and Notake, Y., 1972, Establishment of a cell line of human endometrial adenocarcinoma in vitro, Am. J. Obstet. Gynecol. 114:1012.
Levan, A., 1956, Chromosome studies on some human tumors and tissues of normal origin, grown in vivo and in vitro at the Sloan—Kettering Institute, Cancer 9: 648.
Levan, A., 1967, Some current problems of cancer cytogenetics, Hereditas 57: 343.
Levan, A., 1969, Chromosome abnormalities and carcinogenesis, in: Handbook of Molecular Cytology ( A. Lima-de-Faria, ed.), pp. 717–731, North-Holland, Amsterdam and London.
Levan, A., 1969, Chromosome abnormalities and carcinogenesis, in: Handbook of Molecular Cytology ( A. Lima-de-Faria, ed.), pp. 717–731, North-Holland, Amsterdam and London.
Makino, S., Kikuchi, Y., Sasaki, M. S., Sasaki, M., and Yoshida, M., 1962, A further survey of the chromosomes in the Japanese, Chromosome 13:148.
Makino, S., Sofuni, T., and Mitani, M., 1965, Cytological studies on tumors. XLIII. A chromosome condition in effusion cells from a patient with neuroblastoma, Gann 56: 127.
Manolov, G., and Manolova, Y., 1972, Marker band in one chromosome 14 from Burkitt lymphomas, Nature (Lond.) 237: 33.
Manolov, G., Manolova, Y., Levan, A., and Klein, G., 1971a, Fluorescent pattern of apparently normal chromosomes in Burkitt lymphomas, Hereditas 68:160.
Manolov, G., Manolova, Y., Levan, A., and Klein, G., 1971b, Experiments with fluorescent chromosome staining in Burkitt tumors, Hereditas 68: 235.
Mark, J., 1970, Chromosomal characteristics of neurogenic tumors in children, Acta Cytol. 14:510.
Maunoury, R., Arnoult, J., and Vedrenne, C., 1972, Etablissement et caractérisation d’une lignée cellulaire provenant d’un ostéosarcome humain, Pathol. Biol. 20: 369.
McAllister, R. M., Melnyk, J., Finklestein, J. Z., Adams, E. C., and Gardner, M. B., 1969, Cultivation in vitro of cells derived from a human rhabdomyosarcoma, Cancer 24: 520.
McAllister, R. M., Gardner, M. B., Greene, A. E., Bradt, C., Nichols, W. W., and Landing, B. H., 1971, Cultivation in vitro of cells derived from a human osteosarcoma, Cancer 27: 397.
Miles, C. P., 1965, Chromosomes of some heterotransplanted human tumors. I. H.Emb. Rh. No. 1, H.S. No. I, and ME I, J. Natl. Cancer Inst. 34: 103.
Miles, C. P., 1967a, Chromosome analysis of solid tumors. I. Twenty-eight nonepithelial tumors, Cancer 20:1253.
Miles, C. P., 1967b, Chromosome analysis of solid tumors. II. Twenty-six epithelial tumors, Cancer 20:1274.
Miles, C. P., and Wolinska, W., 1973, A comparative analysis of chromosomes and diagnostic cytology in effusions from 58 cancer patients, Cancer 32: 1458.
Miller, O. J., Miller, D. A., Allderdice, P. W., Dev, V. G., and Grewal, M. S., 1971, Quinacrine
fluorescent karyotypes of human diploid and heteroploid cell lines, Cytogenetics 10:338. Miller, O. J., Miller, D., and Warburton, D., 1973, Application of new staining techniques to the study of human chromosomes, Progr. Med. Genet. 9:1.
Moore, A. E., Sabachewsky, L., and Toolan, H. W., 1955, Culture characteristics of four permanent lines of human cancer cells, Cancer 15: 598.
Moore, G. E., and Koike, A., 1964, Growth of human tumor cells in vitro and in vivo, Cancer 17:11.
Moore, G. E., and Sandberg, A. A., 1964, Studies of a human tumor cell line with a diploid karyotype, Cancer 17: 170.
Moorhead, P. S., Human tumor cell line with a quasi-diploid karyotype (RPMI 2650 ), Exp. Cell. Res. 39:190.
Moorhead, P. S., and Weinstein, D., 1966, Cytogenetic alterations during malignant transformation, Rec. Results Cancer Res. 6: 104.
Nelson-Rees, W. A., McAllister, R. M., and Gardner, M. B., 1972, Clonal aspects of the C-type virus-releasing cells of a cultured human rhabdomyosarcoma line (RD 114) in vitro, Nature New Biol. 236:147.
Norrby, K., Eriksson, 0., and Mellgren, J., 1962, Growth, morphology, and karyotype in 2 pairs of human cell strains of malignant and benign origin from the same individuals, Cancer Res. 22: 147.
Norryd, C., 1959, The chromosomes of three human cell strains, Hereditas 45: 449.
Norryd, C., and Fjelde, A., 1963, The chromosomes in the human cancer cell tissue culture line H.Ep No. 2, Cancer Res. 23: 197.
Nowell, P. C., 1965, Chromosome changes in primary tumors, Progr. Exp. Tumor Res. 7: 83.
Oboshi, S., Tsugawa, S., Seido, T., Shimosato, Y., Koide, T., and Ishikawa, S., 1971, A new floating cell line derived from human pulmonary carcinoma of oat cell type, Gann 62: 505.
Paris Conference (1971): Standardization in Human Cytogenetics, 1972, Birth Defects: Original Articles Series, Vol. VIII, No. 7, The National Foundation, New York.
Pattillo, R. A., and Gey, G. 0., 1968, The establishment of a cell line of human hormone-synthesizing trophoblastic cells in vitro, Cancer Res. 28:1231.
Peterson, W. D., Stulberg, C. S., and Simpson, W. F., 1970, A permanent heteroploid human cell line with type B glucose-6-phosphate dehydrogenase, Proc. Soc. Exp. Biol. Med. 136: 1187.
Petit, P., Verhest, A., Lecluse v. d. Bilt, F., and Jongsma, A., 1972, The chromosomes of EB virus-positive Burkitt cell line P3J.HR1K studied by the fluorescent staining technique, Pathol. Eur. 7: 17.
Petursson, G., and Fogh, J., 1963, The chromosomes of primary human amnion cells and FL cells, Cancer Res. 23: 1021.
Plata, E. J., Aoki, T., Robertson, D. D., Chu, E. W., and Gerwin, B. I., 1973, An established cultured cell line (HBT-39) from human breast carcinoma, J. Natl. Cancer Inst. 50: 849.
Pontén, J., and Saksela, E., 1967, Two established in vitro cell lines from human mesenchymal tumours, Int. J. Cancer 2: 434.
Prasad, K. N., Mandal, B., and Kumar, S., 1973, Demonstration of cholinergic cells in human neuroblastoma and ganglioneuroma, J. Pediat. 82: 677.
Puck, T. T., and Fisher, H. W., 1956, Genetics of somatic mammalian cells. I. Demonstration of the existence of mutants with different growth requirements in a human cancer cell strain (HeLa), J. Exp. Med. 104: 427.
Puck, T. T., Cieciura, S. J., and Robinson, A., 1958, Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects, J. Exp. Med. 108: 945.
Rigby, C. C., and Franks, L. M., 1970, A human tissue culture cell line from a transitional cell tumour of the urinary bladder: Growth, chromosome pattern and ultrastructure, Brit. J. Cancer 24: 746.
Romsdahl, M. M., and Hsu, T. C., 1967, Establishment and biologic properties of human malignant melanoma cell lines grown in vitro, Surg..Forum 18: 78.
Romsdahl, M. M., and Hsu, T. C., 1972, Establishment and characterization of human malignant melanoma cell lines grown in vitro, in: Pigmentation: Its Genesis and Biologic Control (V. Riley, ed.), pp. 461–477, Appleton-Century-Crofts, New York.
Ruddle, F. H., Berman, L., and Stulberg, C. S., 1958, Chromosome analysis of five long-term cell culture populations derived from nonleukemic human peripheral blood (Detroit strains), Cancer Res. 18: 1048.
Saksela, E., and Moorhead, P. S., 1963, Aneuploidy in the degenerative phase of serial cultivation of human cell strains, Proc. Natl. Acad. Sci. 50: 390.
Sandberg, A. A., and Hossfeld, D. K., 1970, Chromosomal abnormalities in human neoplasia, Ann. Rev. Med. 21: 379.
Sandberg, A. A., Yamada, K., Kikuchi, Y., and Takagi, N., 1967, Chromosomes and causation of human cancer and leukemia. III. Karyotypes of cancerous effusions, Cancer 20: 1099.
Sandberg, A. A., Sakurai, M., and Holdsworth, R. N., 1972, Chromosomes and causation of human cancer and leukemia. VIII. DMS chromosomes in a neuroblastoma, Cancer 29: 1671.
Seabright, M., 1971, A rapid banding technique for human chromosomes, Lancet 2: 971.
Shein, H. M., and Enders, J. F., 1962, Multiplication and cytopathogenicity of simian vacuolating virus 40 in cultures of human tissue, Proc. Soc. Exp. Biol. Med. 109: 495.
Spengler, B. A., Biedler, J. L., Helson, L., and Freedman, L. S., 1973, Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells established in vitro, In Vitro 8: 410.
Spriggs, A. I., Boddington, M. M., and Clarke, C. M., 1962, Chromosomes of human cancer cells, Brit. Med. J. 2: 1431.
Spurna, V., and Hill, M., 1966, Chromosomal composition of HeLa and L cells in tissue cultures, Neoplasma 14: 11.
Steel, C. M., 1971, Non-identity of apparently similar chromosome aberrations in human lymphoblastoid cell lines, Nature (Lond.) 233: 555.
Sykes, J. A., Whitescarver, J., Jernstrom, P., Nolan, J. F., and Byatt, P., 1970, Some properties of a new epithelial line of human origin, J. Natl. Cancer Inst. 45: 107.
Syverton, J. T., 1957, Comparative studies of normal and malignant human cells in continuous culture, N.Y. Acad. Sci. Spec. Publ. 5: 331.
Syverton, J. T., and McLaren, L. C., 1956, Stable and pure line human cells in continuous culture for studies of polioviruses, Am. J. Pathol. 32: 640.
Tiepolo, L., and Zuffardi, 0., 1973, Identification of normal and abnormal chromosomes in tumor cells, Cytogenet. Cell Genet. 12: 8.
Tjio, J. H., and Levan, A., 1956, The chromosome number of man, Hereditas 42: 1.
Tjio, J. H., and Puck, T. T., 1958, Genetics of somatic mammalian cells. II. Chromosomal constitution of cells in tissue culture, J. Exp. Med. 108: 259.
Todaro, G. J., and Aaronson, S. A., 1968, Human cell strains susceptible to focus formation by human adenovirus type 12, Proc. Natl. Acad. Sci. 61: 1272.
Toolan, H., 1954, Transplantable human neoplasms maintained in cortisone-treated laboratory animals: H.S. No. 1, H.Ep. No. 1, H.Ep. No. 2, H.Ep. No. 3, and H.Emb. Rh. No. 1, Cancer Res. 14: 660.
Trujillo, J. M., Drewinko, B., Lichtiger, B., Ahearn, M. J., and Cork, A., 1972, Establishment in vitro of a human neurogenic sarcoma, Cancer Res. 32: 1066.
Tumilowicz, J. J., Nichols, W. W., Cholon, J. J., and Greene, A. E., 1970, Definition of a continuous human cell line derived from neuroblastoma, Cancer Res. 30: 2110.
Ugryumov, E. P., and Tsoneva-Maneva, M. T., 1963, Karyotype study of monolayer culture of human stomach cancer cells, Byul. Eksperim. Biol. Med. 55: 93.
Vogt, M., 1959, A study of the relationship between karyotype and phenotype in cloned lines of strain HeLa, Genetics 44: 1257.
Wakonig-Vaartaja, T., Helson, L., Baren, A., Koss, L. C., and Murphy, M. L., 1971, Cytogenetic observations in children with neuroblastoma, Pediatrics 47: 839.
Walker, J. R., 1973, Identification and misidentification of the chromosomes of heteroploid cell lines, J. Natl. Cancer Inst. 51: 1113.
Weinstein, D., and Moorhead, P. S., 1965, Karyology of permanent human cell line, W-18VA2, originated by SV40 transformation, J. Cell. Comp. Physiol. 65: 85.
Weinstein, D., and Moorhead, P. S., 1967, The relation of karyotypic change to loss of contact inhibition of division in human diploid cells after SV40 injection, J. Cell Physiol. 69: 367.
White, L., and Cox, D., 1967, Chromosome changes in rhabdomyosarcoma during recurrence and in cell culture, Brit. J. Cancer 21: 684.
Yamada, K., Takagi, N., and Sandberg, A. A., 1966, Chromosomes and causation of human cancer and leukemia. II. Karyotypes of human solid tumors, Cancer 19: 1879.
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Biedler, J.L. (1975). Chromosome Abnormalities in Human Tumor Cells in Culture. In: Fogh, J. (eds) Human Tumor Cells in Vitro . Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-1647-4_13
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