Time-resolved Spectroscopy of Tryptophyl Fluorescence in Proteins: Application to Yeast 3-Phosphoglycerate Kinase

  • J. P. Privat
  • Ph. Wahl
Part of the NATO Advanced Science Institutes Series book series (NSSA, volume 69)


The emission of protein solutions, irradiated at 295nm, is essentially due to the indole chromophore. Since the physical properties of this emission are highly sensitive to the environment, the fluorescence of tryptophan residues has frequently been used to detect protein conformation changes caused by ligand binding, solvent perturbation or temperature changes.


Emission Wavelength Fluorescence Lifetime Tryptophan Residue Phosphoglycerate Kinase Continuous Excitation 
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Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • J. P. Privat
  • Ph. Wahl

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