Time-resolved Spectroscopy of Tryptophyl Fluorescence in Proteins: Application to Yeast 3-Phosphoglycerate Kinase
The emission of protein solutions, irradiated at 295nm, is essentially due to the indole chromophore. Since the physical properties of this emission are highly sensitive to the environment, the fluorescence of tryptophan residues has frequently been used to detect protein conformation changes caused by ligand binding, solvent perturbation or temperature changes.
KeywordsEmission Wavelength Fluorescence Lifetime Tryptophan Residue Phosphoglycerate Kinase Continuous Excitation
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