Time-resolved Spectroscopy of Tryptophyl Fluorescence in Proteins: Application to Yeast 3-Phosphoglycerate Kinase

  • J. P. Privat
  • Ph. Wahl
Part of the NATO Advanced Science Institutes Series book series (NSSA, volume 69)

Abstract

The emission of protein solutions, irradiated at 295nm, is essentially due to the indole chromophore. Since the physical properties of this emission are highly sensitive to the environment, the fluorescence of tryptophan residues has frequently been used to detect protein conformation changes caused by ligand binding, solvent perturbation or temperature changes.

Keywords

Emission Wavelength Fluorescence Lifetime Tryptophan Residue Phosphoglycerate Kinase Continuous Excitation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • J. P. Privat
  • Ph. Wahl

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