The Excited-State Deprotonation of the Indole N-H of Tryptophan

  • A. G. Szabo
Part of the NATO Advanced Science Institutes Series book series (NSSA, volume 69)


Recently,1 we presented a rationalisation of the dual exponential decay kinetics (τ1 = 3.1 ns, τ2 = 0.5 ns) of the fluorescence of aqueous solutions of tryptophan (pH 7). In order to study the effect of protonation of the α-amino group on these decay kinetics, we measured the fluorescence decay of tryptophan at pH 10.5 (O.1M carbonate buffer), a pH where the α-amino group (pK = 9.3) would largely be unprotonated.


Fluorescence Spectrum Fluorescence Decay Decay Kinetic Dual Exponen Double Exponential Decay 
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    A.G. Szabo & D.M. Rayner, J.Amer.Chem.Soc. 102 554 (1980)CrossRefGoogle Scholar
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    E. Vanderdonckt, Bull.Soc.Chim.Belges 78 69 (1969)Google Scholar
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    J.W. Bridges & R.T. Williams, Biochem.J. 107 225 (1968)Google Scholar
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    L. Brand & W.R. Laws, earlier contribution in this volume.Google Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • A. G. Szabo

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