Measurement of Brownian Correlation Times by Pulse Fluorometry
Fluorescence anisotropy decays are generally measured with a pulse fluorometer, determining fluorescence decays by the single photo-electron method,1–3 which has been described here in several earlier contributions. A schematic of such an apparatus devoted to depolarization measurements is shown in Figure 1.
KeywordsCorrelation Time Fluorescence Anisotropy Transient Fluorescence Anisotropy Decay Statistical Error Estimate
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- 1.Ph. Wahl, Nanosecond pulsefluorimetry, in New Techniques in Biophysics and Cell Biology, R. Pain and B. Smith, eds., Wiley, New York, Volume 2, 1975, pp. 233–285.Google Scholar
- 2.J. Yguerabide, in Methods in Enzymology, C.H.W. Hirs and S.N. Timasheff, eds., Academic Press, New York, Volume 26, Part C, 1972, pp. 498–578.Google Scholar
- 3.M.G. Badea and L. Brand, in Methods in Enzymology, C.H.W. Hirs and S.N. Timasheff, eds., Academic Press, New York, Volume 61, 1979, pp. 378–425.Google Scholar
- 5.J.A. Miehe, G. Ambard, J. Zampach and A. Coche, IEEE Trans.Nucl. Sci 15, 17 (1970)Google Scholar
- 7.A.E.W. Knight and B.K. Selinger, Spectrochim.Acta 27A, 1223 (1971)Google Scholar
- 9.P.R. Bevington, Data Reduction and Error Analysis for the Physical Sciences, McGraw-Hill, New York, 1969.Google Scholar