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Measurement of Brownian Correlation Times by Pulse Fluorometry

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Time-Resolved Fluorescence Spectroscopy in Biochemistry and Biology

Part of the book series: NATO Advanced Science Institutes Series ((NSSA,volume 69))

Abstract

Fluorescence anisotropy decays are generally measured with a pulse fluorometer, determining fluorescence decays by the single photo-electron method,1–3 which has been described here in several earlier contributions. A schematic of such an apparatus devoted to depolarization measurements is shown in Figure 1.

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References

  1. Ph. Wahl, Nanosecond pulsefluorimetry, in New Techniques in Biophysics and Cell Biology, R. Pain and B. Smith, eds., Wiley, New York, Volume 2, 1975, pp. 233–285.

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Authors
  1. Ph. Wahl
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Editors and Affiliations

  1. University of Salford, Salford, UK

    R. B. Cundall

  2. Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester, UK

    R. E. Dale

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© 1983 Springer Science+Business Media New York

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Wahl, P. (1983). Measurement of Brownian Correlation Times by Pulse Fluorometry. In: Cundall, R.B., Dale, R.E. (eds) Time-Resolved Fluorescence Spectroscopy in Biochemistry and Biology. NATO Advanced Science Institutes Series, vol 69. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-1634-4_27

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  • DOI: https://doi.org/10.1007/978-1-4757-1634-4_27

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-1636-8

  • Online ISBN: 978-1-4757-1634-4

  • eBook Packages: Springer Book Archive

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