Abstract

After a brief review of the history of pulse fluorometry, various aspects of the single-photon technique will be described and discussed. The main aim is to provide a basis for the more specialized contributions to follow. Topics to be discussed will include pulsed light sources, detection systems, general theory of distortions, and time-resolved spectroscopy. Selected examples will be provided to illustrate the versatile nature of pulse fluorometry, and some comments will be made regarding the relative merits of pulse vs. phase fluorometry.

Keywords

Decay Curve Fluorescence Decay Streak Camera Flash Lamp Measured Fluorescence Decay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    E. Gaviola, Z.Phys. 35 748 (1976)Google Scholar
  2. 2.
    R.K. Swank and W.L. Buck, Rev.Sci.Instrum. 26 15 (1955)Google Scholar
  3. 3.
    S.S. Brody, Rev.Sci.Instrum. 28 1021 (1957)Google Scholar
  4. 4.
    J. Malmberg, Rev.Sci.Instrum. 28 1027 (1957)Google Scholar
  5. 5.
    W.R. Bennett,Jr., A. Jaran and E.A. Ballik, Bull.Amer.Phys. Soc. 5 496 (1960)Google Scholar
  6. 6.
    S. Heron, R. McWhirter and E.H. Rhoderic, Proc.Roy.Soc.(London) A234 565 (1956)Google Scholar
  7. 7.
    L.M. Bollinger and G.E. Thomas, Rev.Sci.Instrum. 32 1044 (1961)Google Scholar
  8. 8.
    Y. Koechlin and A. Raviart, Nucl.Instrum.Methods 29 45 (1964)Google Scholar
  9. 9.
    J. Yguerabide, Rev.Sci.Instrum. 36 1734 (1965)Google Scholar
  10. 10.
    L. Lindqvist, R. Lopez-Delgado, M. Martin and A. Tramer, Proc. Intern.Symp.Synchrotron Rad. Users, Daresbury, Jan. (1973); see also, Opt.Commun. 10 283 (1974)ADSCrossRefGoogle Scholar
  11. 11.
    F.E. Lytle and M.S. Kelsey, Analyt.Chem. 46 855 (1974)Google Scholar
  12. 12.
    J.M. Harris, L.M. Gray, M.J. Pelletier and F.E. Lytle, Mol. Photochem. 8 161 (1977)Google Scholar
  13. 13.
    For an excellent review, see Topics in Applied Physics, S.L. Shapiro, ed., Volume 18, Springer — Verlag, Berlin, 1977Google Scholar
  14. 14.
    R.G. Bennett, Rev.Sci.Instrum. 31 1275 (1960)Google Scholar
  15. 15.
    H. Dreeskamp, A. Ghosh and M. Burton, Rev.Sci.Instrum. 32 304 (1961)Google Scholar
  16. 16.
    W. Rothman, F. Hirayama and S. Lipsky, J.Chem.Phys. 58 1300 (1973), and references contained therein to earlier work.Google Scholar
  17. 17.
    W.R. Ware and R.L. Lyke, Chem.Phys.Lett. 24 195 (1975); W.R. W.re and D.V. O’Connor, Chem.Phys.Lett. 62 595 (1979)Google Scholar
  18. 18.
    W.R. Ware, in Creation and Detection of the Excited State, A.A. Lamola, ed., Volume 1, Marcel Dekker, New York, 1971.Google Scholar
  19. 19.
    A.E. Knight and B.K. Selinger, Aust.J.Chem. 26 1 (1973)Google Scholar
  20. 20.
    Th. Binkert, H.P. Tschanz and P.E. Zinsli, J.Luminescence 5 187 (1972)Google Scholar
  21. 21.
    S.K. Poultney, Adv.Electronics Electron Phys. 31 39 (1972)Google Scholar
  22. 22.
    J. Yguerabide, in Methods in Enzymology, C.H.W. Hirs and S.N. Timasheff, eds., Volume 26, Part C, Academic Press, New York, 1972, pp. 498–578.Google Scholar
  23. 23.
    Ph. Wahl, in New Techniques in Biophysics and Cell Biology, R.H. Pain and B.J. Smith, eds., Volume 2, John Wiley, New York, 1975, pp. 233–285.Google Scholar
  24. 24.
    V.J. Koester and R.M. Dowben, Rev.Sci.Instrum. 49 1186 (1978)Google Scholar
  25. 25.
    K.G. Spears, L.E. Cramer and L.D. Hoffland, Rev.Sci.Instrum. 49 255 (1978)Google Scholar
  26. 26.
    W.R. Ware, R.L. Lyke, J.C. Andre and R. Lopez-Delgado, Appl. Optics 13 1355 (1979)Google Scholar
  27. 27.
    W.R. Ware, R. Lopez-Delgado and R.L. Lyke, unpublished results.Google Scholar
  28. 28.
    O.J. Steingraber and I.B. Berlman, Rev.Sci.Instrum. 34 524 (1963)Google Scholar
  29. 29.
    J.S. Gethner and G.W. Flynn, Rev.Sci.Instrum. 46 586 (1975)Google Scholar
  30. 30.
    G. Bertolini, V. Mandl, A. Rota and M. Cocchi, Nucl.Instrum. Methods 42 109 (1966)Google Scholar
  31. 31.
    F. Callagaris, P. Cinti, I. Gabrielli, R. Giacomich and R. Musetti, Nucl.Instrum.Methods 157 611 (1978)Google Scholar
  32. 32.
    C. Lewis and W.R. Ware, Rev.Sci.Instrum. 44 107 (1973)Google Scholar
  33. 33.
    D.M. Rayner, A.E. McKinnon and A.G. Szabo, Rev.Sci.Instrum. 48 1050 (1977)Google Scholar
  34. 34.
    Ph.Wahl, J.C. Auchet and B. Donzel, Rev.Sci.Instrum. 45 28 (1974)Google Scholar
  35. 35.
    G. Present and D.B. Scarl, Rev.Sci.Instrum. 41 771 (1976)Google Scholar
  36. 36.
    C.C. Davis and T.A. King, J.Phys.A. 3 101 (1970); Rev.Sci. Instrum. 41 407 (1970)Google Scholar
  37. 37.
    P.B. Coates, Rev.Sci.Instrum. 43 1855 (1972)Google Scholar
  38. 38.
    G.R. Haugen, B.W. Wallin and F.E. Lytle, Rev.Sci.Instrum. 50 64 (1979)Google Scholar
  39. 39.
    M. Willams and W.J. Sandle, J.Phys.E. 3 741, 1028 (1970)Google Scholar
  40. 40.
    F.A. Johnson, R. Jones, T.P. McLean and E.R. Pike, Phys.Rev. Lett. 16 589 (1966)Google Scholar
  41. 41.
    C. Holzapfel, Rev.Sci.Instrum. 45 894 (1974)Google Scholar
  42. 42.
    B.I. Cantor and M.C. Teich, J.Opt.Soc.Amer. 65 786 (1975)Google Scholar
  43. 43.
    D.E. Donohue and R.C. Stern, Rev.Sci.Instrum 43 791 (1972)Google Scholar
  44. 44.
    W.R. Ware, D.V. O’Connor and J.C. Andre, J.Phys.Chem. 83 1333 (1979)Google Scholar
  45. 45.
    W.R. Ware, L.J. Doemeny and T.L. Nemzek, J.Phys.Chem. 77 2038 (1973)Google Scholar
  46. 46.
    W.R. Ware, D.V. O’Connor, J.C. Andre and L.M. Vincent, J.Phys. Chem. 83 2285 (1979).Google Scholar
  47. 47.
    J.H. Easter, R.P. DeToma and L. Brand, Biophys.J. 16 571 (1976)Google Scholar
  48. 48.
    W.R. Ware, S.K. Lee, G.J. Brant and P. Chow, J.Chem.Phys. 54 4729 (1971)Google Scholar
  49. 49.
    W.R. Ware and S.K. Chakrabarti, J.Chem.Phys. 55 5494 (1971)Google Scholar
  50. 50.
    C.A. Parker, Luminescence of Solutions, Elsevier, Amsterdam, 1968.Google Scholar
  51. 51.
    R.D. Spencer and G. Weber, J.Chem.Phys. 52 1654 (1970)Google Scholar
  52. 52.
    R.E. Dale, J.Luminescence 18/19 120 (1979)Google Scholar
  53. 53.
    Ph. Wahl, in Biochemical Fluorescence: Concepts, R.F. Chen and H. Edelhoch, eds., Volume 1, Marcel Dekker Inc., New York, 1975, pp. 1–41.Google Scholar
  54. 54.
    M. Shinitzky, J.Chem.Phys. 56 5979 (1972)Google Scholar
  55. 55.
    W.R. Ware and P.T. Cunningham, J.Chem.Phys. 44 3826 (1965)Google Scholar
  56. 56.
    W.R. Ware and R.L. Lyke, Rev.Sci.Instrum. 48 320 (1977)Google Scholar
  57. 57.
    R.L. Lyke and W.R. Ware, Chem.Phys.Lett. 24 195 (1974)Google Scholar
  58. 58.
    B.K. Selinger and W.R. Ware, J.Chem.Phys. 53 3860 (1970)Google Scholar
  59. 59.
    W.R. Ware, A.M. Garcia, C.S. Parmenter, M.D. Schun and K.Y. Tang, Chem.Phys. 17 377 (1976)Google Scholar
  60. 60.
    C.S. Parmenter, K.Y. Tang and W.R. Ware, Chem.Phys. 17 359 (1976)Google Scholar
  61. 61.
    W.R. Ware and T.L. Nemzek, J.Chem.Phys. 62 477 (1975)Google Scholar
  62. 62.
    W.R. Ware and D.V. O’Connor, J.Amer.Chem.Soc. 98 4706 (1976)Google Scholar
  63. 63.
    A.M. Halpern and W.R. Ware, J.Chem.Phys. 53 1969 (1970)Google Scholar
  64. 64.
    K. Hara, P. de Mayo, W.R. Ware, A.C. Weedon, G.S.K. Wong and K.C. Wu, Chem.Phys.Lett. 69 105 (1980)Google Scholar
  65. 65.
    M. Hauser and G. Heidt, Rev.Sci.Instrum. 46 470 (1975)Google Scholar
  66. 66.
    E. Gratton and R. Lopez-Delgado, Rev.Sci.Instrum. 50 789 (1979)Google Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • W. R. Ware

There are no affiliations available

Personalised recommendations