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Analysis of Pulse Fluorometry Data by Laplace Transforms

  • A. Gafni
Part of the NATO Advanced Science Institutes Series book series (NSSA, volume 69)

Abstract

The fluorescence decay curves observed in experiments performed using pulse techniques are distorted versions of the fluorescence response functions, due to the finite duration of the excitation pulses and to the response time of the instrument. The observed fluorescence decay is related to the impulse response (i.e. the fluorescence decay which would have been obtained if the observed lamp flash was infinitely short) by convolution:
(1)
I(t) is the observed fluorescence decay function, E(t) is the observed excitation pulse and i(t) is the impulse response function.

Keywords

Decay Curve Fluorescence Decay Impulse Response Function Lamp Flash Fluorescence Decay Curve 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    I. Isenberg and R. D. Dyson, The Analysis of Fluorescence Decay by a Method of Moments, Biophys. J. 9 1337 (1969).CrossRefGoogle Scholar
  2. 2.
    I. Isenberg, R. D. Dyson and R. Hanson, Studies on the Analysis of Fluorescence Decay Data by the Method of Moments, Biophys. J. 13 1090 (1973)CrossRefGoogle Scholar
  3. 3.
    W. R. Ware, L. J. Doemeny and T. L. Nemzek, Deconvolution of Fluorescence and Phosphorescence Decay Curves. A Least-Squares Method, J.Phys.Chem. 77 2038 (1973)CrossRefGoogle Scholar
  4. 4.
    A. Grinvald and I. Z. Steinberg, On the Analysis of Fluorescence Decay Kinetics by the Method of Least-Squares, Analyt. Biochem 59 583 (1974)Google Scholar
  5. 5.
    B. Valeur, Analysis of Time Dependent Fluorescence Experiments by the Method of Modulating Functions with Special Attention to Pulse Fluorometry, Chem. Phys. 30 85 (1978)ADSCrossRefGoogle Scholar
  6. 6.
    W. P. Helman, Analysis of Very Fast Transient Luminescence Behaviour, Int.J. Radiat. Phys. Chem. 3 283 (1971)CrossRefGoogle Scholar
  7. 7.
    R. A. Kashnow and J. A. Sousa, Ultraviolet Laser-Excited Fluorescence Decay Measurements by Convolution Techniques, J.Appl.Phys. 42 2128 (1971)ADSCrossRefGoogle Scholar
  8. 8.
    M. Almgren, Analysis of Pulse Fluorometry Data of Complex Systems, Chemica Scripta, 3 145 (1973)Google Scholar
  9. 9.
    M. S. Henry and W. P. Helman, Fluorescence Lifetimes of Saturated Hydrocarbons, J.Chem.Phys. 56 5734 (1972)ADSCrossRefGoogle Scholar
  10. 10.
    A. Gafni, R. L. Modlin and L. Brand, Analysis of Fluorescence Decay Curves by Means of the Laplace Transformation, Biophys.J. 15 263 (1975)ADSCrossRefGoogle Scholar
  11. 11.
    M. L. Boas, Mathematical Methods in the Physical Sciences, Wiley, New York, 1966.zbMATHGoogle Scholar
  12. 12.
    A. E. McKinnon, A. G. Szabo and D. R. Miller, The Deconvolution of Photoluminescence Data, J.Phys.Chem. 81 1564 (1977).CrossRefGoogle Scholar
  13. 13.
    D. V. O’Connor, W. R. Ware and J. C. Andre, Deconvolution of Fluorescence Decay Curves. A Critical Comparison of Techniques, J.Phys.Chem. 83 1333 (1979)CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • A. Gafni

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