Analysis of Pulse Fluorometry Data by Laplace Transforms

  • A. Gafni
Part of the NATO Advanced Science Institutes Series book series (NSSA, volume 69)


The fluorescence decay curves observed in experiments performed using pulse techniques are distorted versions of the fluorescence response functions, due to the finite duration of the excitation pulses and to the response time of the instrument. The observed fluorescence decay is related to the impulse response (i.e. the fluorescence decay which would have been obtained if the observed lamp flash was infinitely short) by convolution:
I(t) is the observed fluorescence decay function, E(t) is the observed excitation pulse and i(t) is the impulse response function.


Decay Curve Fluorescence Decay Impulse Response Function Lamp Flash Fluorescence Decay Curve 
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© Springer Science+Business Media New York 1983

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  • A. Gafni

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