Enzymes in Non-Aqueous Systems

  • Marcel Waks
Part of the Progress in Mathematics book series (NSSA)


Enzymes active in non-aqueous systems have been also designated as “dry enzymes”1. The notion does not imply the absence of any water at all in the reaction medium, however, since enzymatic activity requires at least 0.2 g of water per gram of protein. This amount of water, determined in sequential rehydrat,on experiments on solid vitrified samples of lysozyme by Careri et al2, is tightly bound to the surface of the protein and can be considered to be a different solvent from bulk water. Indeed its physicochemical properties are comparable to “biological water”3 also present at membrane interfaces and which plays a key role in the control of many important biological processes.


Reverse Micelle Micellar Solution Rotational Diffusion Polar Head Group Rotational Correlation Time 
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Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Marcel Waks
    • 1
  1. 1.Unité Associée 586 du CNRSUniversité René DescartesParis Cedex 06France

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