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The Fluorescence Properties of Aromatic Amino Acids: Their Role in the Understanding of Enzyme Structure and Dynamics

  • Chapter
The Enzyme Catalysis Process

Part of the book series: Progress in Mathematics ((NSSA))

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Abstract

Fluorescence spectroscopy has been extensively used in studies of enzymes and proteins (1–5). It has been shown to provide insights into important aspects of the interrelationships of enzyme structure, function and dynamics. The reasons for the wide applicability of fluorescence spectroscopy are due to several factors. Fluorescence has a high degree of sensitivity allowing one to work at low concentrations typical of “in vivo” conditions. Since the process depends on the absorption and emission of light energy it is selective, probing only those molecular subunits which have appropriate chromophoric properties. A wide vartiety of information is available including the study of inter and intramolecular interactions; the local environment of the fluorescent chromOphore; conformational heterogeneity; the rates of diffusional processes; the dynamics of the flexibility of the protein segments; and the kinetics of enzymatic processes. These studies have been facilitated by the relative ease of obtaining high quality fluorescence measurements using commercially available instruments.

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Author information

Authors and Affiliations

  1. Division of Biological Sciences Protein Biochemistry and Spectroscopy, National Research Council, Ottawa, Canada, K1A OR6

    Arthur G. Szabo

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  1. Arthur G. Szabo
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Editor information

Editors and Affiliations

  1. Dept. of Chemistry, University of Glasgow, G12 8QQ, Glasgow, Scotland, UK

    Alan Cooper

  2. Istituto di Chimica Quantistica ed Energetica Molecolare, CNR, Via Risorgimento 35, I-56100, Pisa, Italy

    Julien L. Houben  & Lisa C. Chien  & 

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© 1989 Springer Science+Business Media New York

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Szabo, A.G. (1989). The Fluorescence Properties of Aromatic Amino Acids: Their Role in the Understanding of Enzyme Structure and Dynamics. In: Cooper, A., Houben, J.L., Chien, L.C. (eds) The Enzyme Catalysis Process. Progress in Mathematics. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-1607-8_10

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  • DOI: https://doi.org/10.1007/978-1-4757-1607-8_10

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-1609-2

  • Online ISBN: 978-1-4757-1607-8

  • eBook Packages: Springer Book Archive

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