Binding Ligands to Receptors: Additional Comments

  • Alexander Levitzki
Part of the NATO ASI Series book series (NSSA, volume 72)


Radioligand receptor assays enable the investigators to study directly the number of receptors in a tissue and the type of binding displayed by different ligands. In this chapter we shall address mainly the question what can we learn about receptors and their mode of action from ligand binding studies.


Nicotinic Receptor Binding Experiment Radioligand Binding Assay High Affinity Binding Site Negative Cooperativity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    A. Levitzki, Quantitative aspects of ligand binding to receptors, in: Cellular Receptors for Hormones and Neurotransmitters, D. Schulster and A. Levitzki, Wiley $ Sons, pp. 9–28, 1980.Google Scholar
  2. 2.
    S.P. Colowick and F.C. Womack (1969) Binding of diffusable molecules by macromolecules: Rapid measurement by rate dialysis, J. Biol. Chem. 244: 774.PubMedGoogle Scholar
  3. 3.
    B. Desbuquois and G. Aurbach (1971) Use of polyethylene glycol to separate free and antibody-bound peptide hormones in radioimmunoassays, J. Clin. Endocrinol. 33: 732.CrossRefGoogle Scholar
  4. 4.
    A. Levitzki (1983) in:“Receptor Binding and Receptor-to-Signal Coupling”, in preparation.Google Scholar
  5. 5.
    Y.I. Henis and A. Levitzki (1979) Ligand competition curves as diagnostic tool for delineating the nature of site-site interactions: Theory, Eur. J. Biochem. 102: 449.PubMedCrossRefGoogle Scholar
  6. 6.
    Y.I. Henis and A. Levitzki (1980) The sequential nature of the negative cooperativity in rabbit muscle glyceraldehyde-3-phosphate dehydrogenase, Eur. J. Biochem. 115: 59.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • Alexander Levitzki
    • 1
  1. 1.Department of Biological ChemistryThe Hebrew University of JerusalemJerusalemIsrael

Personalised recommendations