Abstract
Radioligand receptor assays enable the investigators to study directly the number of receptors in a tissue and the type of binding displayed by different ligands. In this chapter we shall address mainly the question what can we learn about receptors and their mode of action from ligand binding studies.
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Keywords
- Nicotinic Receptor
- Binding Experiment
- Radioligand Binding Assay
- High Affinity Binding Site
- Negative Cooperativity
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References
A. Levitzki, Quantitative aspects of ligand binding to receptors, in: Cellular Receptors for Hormones and Neurotransmitters, D. Schulster and A. Levitzki, Wiley $ Sons, pp. 9–28, 1980.
S.P. Colowick and F.C. Womack (1969) Binding of diffusable molecules by macromolecules: Rapid measurement by rate dialysis, J. Biol. Chem. 244: 774.
B. Desbuquois and G. Aurbach (1971) Use of polyethylene glycol to separate free and antibody-bound peptide hormones in radioimmunoassays, J. Clin. Endocrinol. 33: 732.
A. Levitzki (1983) in:“Receptor Binding and Receptor-to-Signal Coupling”, in preparation.
Y.I. Henis and A. Levitzki (1979) Ligand competition curves as diagnostic tool for delineating the nature of site-site interactions: Theory, Eur. J. Biochem. 102: 449.
Y.I. Henis and A. Levitzki (1980) The sequential nature of the negative cooperativity in rabbit muscle glyceraldehyde-3-phosphate dehydrogenase, Eur. J. Biochem. 115: 59.
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© 1984 Springer Science+Business Media New York
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Levitzki, A. (1984). Binding Ligands to Receptors: Additional Comments. In: Cattabeni, F., Nicosia, S. (eds) Principles and Methods in Receptor Binding. NATO ASI Series, vol 72. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-1577-4_3
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DOI: https://doi.org/10.1007/978-1-4757-1577-4_3
Publisher Name: Springer, Boston, MA
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