Enzyme Studies with the Mössbauer Effect

  • R. Cooke
Conference paper


We have used the Mössbauer effect to study the found in the active centers of two enzymes, cytochome c and putidaredoxin. The temperature dependence and magnitude of the quadrupole splitting of cytochome c are interpreted in terms of molecular orbitals. The Mössbauer spectra of putidaredoxin show that the two iron atoms of that molecule are identical and one electron reduces both. The Mössbauer data are correlated with the results of electron-spin resonance and magnetic susceptibility experiments and are discussed in terms of models of the active site.


Iron Atom Isomer Shift Sodium Nitroprusside Quadrupole Splitting Electric Field Gradient 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. L Y. Disatnik, D. Fainstein, and H. J. Lipkin, Phys. Rev. 139: 292 (1965).CrossRefGoogle Scholar
  2. 2.
    R. Cooke and P. Debrunner, to be published, J. Chem. Phys., May 15, 1968.Google Scholar
  3. 3.
    G. Lang and W. Marshall, Proc. Phys. Soc. (London) 87: 3 (1966).CrossRefGoogle Scholar
  4. 4.
    R. W. Estabrooke, in: Haematin Enzymes, J. E. Falk, R. Lemberg, and R. K. Morton, Eds. ( Pergamon Press, London, 1961 ), p. 436.Google Scholar
  5. 5.
    F. C. Ruegg and J. J. Spijkerman, Natl. Bur. Std. (U.S.), Misc. Publ., Tech. Note 248: 25 (1964).Google Scholar
  6. 6.
    E. Margoliash and A. Schejter, in: The Proteins, Vol. V, H. Neurath, Ed. ( Academic Press, New York, 1968 ).Google Scholar
  7. 7.
    M. Zerner, M. Gouterman, and K. Hiroshi, Theoret. Chim. Acta 6: 363 (1966).CrossRefGoogle Scholar
  8. 8.
    I. Saimeen and G. Palmer, J. Chem. Phys. 48: 2049 (1968).CrossRefGoogle Scholar
  9. 9.
    P. George, J. Beetlestone, and J. S. Griffith, Rev. Mod. Phys. 36: 441 (1964).CrossRefGoogle Scholar
  10. 10.
    P. George, in: Biopolymer Symposia No. 1, Weissbluth, Ed. ( Interscience Publishers, New York, 1963 ), p. 40.Google Scholar
  11. 11.
    J. S. Griffith, J. Inorg. Nucl. Chem. 2: 1 (1956).CrossRefGoogle Scholar
  12. 12.
    D. W. Cushman, R. L. Tsai, and I. C. Gunsalus, Biochetri. Biophys. Res. Commun. 26: 577 (1967).CrossRefGoogle Scholar
  13. 13.
    J. C. M. Tsibris, R. L. Tsai, and I. C. Gunsalus. Am. Chem. Soc. Abstr. 154: C 204(1967).Google Scholar
  14. 14.
    D. V. DerVartanian, W. H. Orme-Johnson, R. E. Hansen, H. Beinert. R. L. Tsai, J. C. M. Tsibris, R. C. Bartholomaus, and I. C. Gunsalus, Blochenz, Biophys. Res. Comm. 26: 569 (1967).CrossRefGoogle Scholar
  15. 15.
    J. C. M. Tsibris, R. L. Tsai, I. C. Gunsalus, W. H. Orme-Johnson, R. E. Hansen, and H. Beinert, Proc. Nat. Acad. Sci. 59: 959 (1968).CrossRefGoogle Scholar
  16. 16.
    R. Cooke, J. C. M. Tsibris, P. G. Debrunner, R. Tsai, I. C. Gunsalus, and H. Frauenfelder, Proc. Nat, Acad, Sci. 59: 1045 (1968).CrossRefGoogle Scholar
  17. 17.
    H. Brintzinger, G. Palmer, and R. H. Sands, Proc. Natl, Acad. Sci. U.S. 55: 397 (1966).CrossRefGoogle Scholar
  18. 18.
    J. F. Gibson, D. O. Hall, J. H. M. Thornley, and F. R. Whatley, Proc. Natl. Acad. Sci. U.S. 56: 987 (1966).CrossRefGoogle Scholar
  19. 19.
    R. E. Watson and A. J. Freeman, Phys. Rev. 123: 2027 (1961).CrossRefGoogle Scholar
  20. 20.
    W. Kerler, W. Neuwirth, E. Elude, P. Kuhn, and B. Zimmerman, Z. Physik 173: 321 (1963).CrossRefGoogle Scholar
  21. 21.
    G. Foex, Tables de constantes et données numeriques, No. 7 (Gorter and Smits, Paris, 1957 ), p. 73.Google Scholar
  22. 22.
    V. I. Goldanskii, The Mössbauer Effect and Its Application to Chemistry (D. Van Nostrand Co., Princeton, N.J., 1966 ).Google Scholar
  23. 23.
    P. R. Brady, J. F. Duncan, and K. F. Mok, Proc. Roy. Soc. (London) A287: 343 (1965).CrossRefGoogle Scholar
  24. 24.
    K. Luchner and J. Dietl, Z. Physik 176: 261 (1963).CrossRefGoogle Scholar
  25. 25.
    A. J. Bearden, T. H. Moss, R. G. Bartsch, M. A. Cusanovith, in: Non-Heme Iron Proteins, S. A. Pietro, Ed. ( Antioch Press, Yellow Springs, Ohio, 1965 ), p. 87.Google Scholar
  26. 26.
    D. C. Biomstrom, E. Knight, Jr., W. D. Phillips, and J. F. Weihcr, Proc. Natl. Acad. Sci. U.S. 51: 1085 (1964).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1968

Authors and Affiliations

  • R. Cooke
    • 1
  1. 1.Department of PhysicsUniversity of IllinoisUrbanaUSA

Personalised recommendations