Enzyme Studies with the Mössbauer Effect

Cooke, R.

doi:10.1007/978-1-4757-1550-7_7

Enzyme Studies with the Mössbauer Effect

R. Cooke²^nAff3

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Abstract

We have used the Mössbauer effect to study the found in the active centers of two enzymes, cytochome c and putidaredoxin. The temperature dependence and magnitude of the quadrupole splitting of cytochome c are interpreted in terms of molecular orbitals. The Mössbauer spectra of putidaredoxin show that the two iron atoms of that molecule are identical and one electron reduces both. The Mössbauer data are correlated with the results of electron-spin resonance and magnetic susceptibility experiments and are discussed in terms of models of the active site.

Supported by the National Institute of Health, 5T1-GM720 and the U.S. Office of Naval Research under Contract 00014-67-A-0305-0005.

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References

L Y. Disatnik, D. Fainstein, and H. J. Lipkin, Phys. Rev. 139: 292 (1965).
Article CAS Google Scholar
R. Cooke and P. Debrunner, to be published, J. Chem. Phys., May 15, 1968.
Google Scholar
G. Lang and W. Marshall, Proc. Phys. Soc. (London) 87: 3 (1966).
Article CAS Google Scholar
R. W. Estabrooke, in: Haematin Enzymes, J. E. Falk, R. Lemberg, and R. K. Morton, Eds. ( Pergamon Press, London, 1961 ), p. 436.
Google Scholar
F. C. Ruegg and J. J. Spijkerman, Natl. Bur. Std. (U.S.), Misc. Publ., Tech. Note 248: 25 (1964).
Google Scholar
E. Margoliash and A. Schejter, in: The Proteins, Vol. V, H. Neurath, Ed. ( Academic Press, New York, 1968 ).
Google Scholar
M. Zerner, M. Gouterman, and K. Hiroshi, Theoret. Chim. Acta 6: 363 (1966).
Article CAS Google Scholar
I. Saimeen and G. Palmer, J. Chem. Phys. 48: 2049 (1968).
Article Google Scholar
P. George, J. Beetlestone, and J. S. Griffith, Rev. Mod. Phys. 36: 441 (1964).
Article CAS Google Scholar
P. George, in: Biopolymer Symposia No. 1, Weissbluth, Ed. ( Interscience Publishers, New York, 1963 ), p. 40.
Google Scholar
J. S. Griffith, J. Inorg. Nucl. Chem. 2: 1 (1956).
Article CAS Google Scholar
D. W. Cushman, R. L. Tsai, and I. C. Gunsalus, Biochetri. Biophys. Res. Commun. 26: 577 (1967).
Article CAS Google Scholar
J. C. M. Tsibris, R. L. Tsai, and I. C. Gunsalus. Am. Chem. Soc. Abstr. 154: C 204(1967).
Google Scholar
D. V. DerVartanian, W. H. Orme-Johnson, R. E. Hansen, H. Beinert. R. L. Tsai, J. C. M. Tsibris, R. C. Bartholomaus, and I. C. Gunsalus, Blochenz, Biophys. Res. Comm. 26: 569 (1967).
Article CAS Google Scholar
J. C. M. Tsibris, R. L. Tsai, I. C. Gunsalus, W. H. Orme-Johnson, R. E. Hansen, and H. Beinert, Proc. Nat. Acad. Sci. 59: 959 (1968).
Article CAS Google Scholar
R. Cooke, J. C. M. Tsibris, P. G. Debrunner, R. Tsai, I. C. Gunsalus, and H. Frauenfelder, Proc. Nat, Acad, Sci. 59: 1045 (1968).
Article CAS Google Scholar
H. Brintzinger, G. Palmer, and R. H. Sands, Proc. Natl, Acad. Sci. U.S. 55: 397 (1966).
Article CAS Google Scholar
J. F. Gibson, D. O. Hall, J. H. M. Thornley, and F. R. Whatley, Proc. Natl. Acad. Sci. U.S. 56: 987 (1966).
Article CAS Google Scholar
R. E. Watson and A. J. Freeman, Phys. Rev. 123: 2027 (1961).
Article Google Scholar
W. Kerler, W. Neuwirth, E. Elude, P. Kuhn, and B. Zimmerman, Z. Physik 173: 321 (1963).
Article CAS Google Scholar
G. Foex, Tables de constantes et données numeriques, No. 7 (Gorter and Smits, Paris, 1957 ), p. 73.
Google Scholar
V. I. Goldanskii, The Mössbauer Effect and Its Application to Chemistry (D. Van Nostrand Co., Princeton, N.J., 1966 ).
Google Scholar
P. R. Brady, J. F. Duncan, and K. F. Mok, Proc. Roy. Soc. (London) A287: 343 (1965).
Article CAS Google Scholar
K. Luchner and J. Dietl, Z. Physik 176: 261 (1963).
Article CAS Google Scholar
A. J. Bearden, T. H. Moss, R. G. Bartsch, M. A. Cusanovith, in: Non-Heme Iron Proteins, S. A. Pietro, Ed. ( Antioch Press, Yellow Springs, Ohio, 1965 ), p. 87.
Google Scholar
D. C. Biomstrom, E. Knight, Jr., W. D. Phillips, and J. F. Weihcr, Proc. Natl. Acad. Sci. U.S. 51: 1085 (1964).
Article Google Scholar

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R. Cooke (Predoctoral trainee in Biophysics, N.I.H.)
Present address: Medical Center, University of California, San Francisco, California, USA

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Department of Physics, University of Illinois, Urbana, Illinois, USA
R. Cooke (Predoctoral trainee in Biophysics, N.I.H.)

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R. Cooke
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Nuclides and Sources Division, New England Nuclear Corporation, Boston, Massachusetts, USA
Irwin J. Gruverman (Technical Director) (Technical Director)

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Cooke, R. (1968). Enzyme Studies with the Mössbauer Effect. In: Gruverman, I.J. (eds) Mössbauer Effect Methodology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-1550-7_7

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DOI: https://doi.org/10.1007/978-1-4757-1550-7_7
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-1552-1
Online ISBN: 978-1-4757-1550-7
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