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Approaches to the Study of the Function and Activity of Bone Regulatory Factors: Established and Potential Methods

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Bone Regulatory Factors

Part of the book series: NATO ASI Series ((NSSA,volume 184))

Abstract

The aim of this paper is to review methodological approaches which can either be used to explore the biological activities of bone regulatory factors or be used to measure the amount of a particular factor. The discrimination between explore and measure needs to be made at the outset because practical and scientific considerations for each of these aspects can, at times, appear to be mutually exclusive. This is not intended to be a comprehensive review of methodologies and we do not propose to compare and contrast individual methods for individual substances; instead, we aim to review the rationale of choosing, or developing, appropriate laboratory techniques. We will also propose potential methods, based on in situ biochemistry, which are expected to make a major research contribution to the study of hone regulatory factors. Selection of the most appropriate methodologies requires an objective assessment of the scientific questions to be asked and the relevance of the experimental answers likely to be obtained.

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  1. Joan M. Zanelli

    Present address: Bone Disease Research Group, Clinical Research Centre, Harrow, HA1 3UJ, UK

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  1. National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, EN6 3QG, UK

    Joan M. Zanelli

  2. Bone Growth and Metabolism Unit, Rowett Research Institute, Aberdeen, AB2 9SB, UK

    Nigel Loveridge

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  1. Joan M. Zanelli
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  1. University of Milan, Milan, Italy

    Antonio Pecile (Professor of Pharmacology) (Professor of Pharmacology)

  2. Biophysics and Chemistry of Macromolecules, University of Trieste, Trieste, Italy

    Benedetto de Bernard (Head of the Dept. of Biochemistry) (Head of the Dept. of Biochemistry)

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Zanelli, J.M., Loveridge, N. (1990). Approaches to the Study of the Function and Activity of Bone Regulatory Factors: Established and Potential Methods. In: Pecile, A., de Bernard, B. (eds) Bone Regulatory Factors. NATO ASI Series, vol 184. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-1508-8_11

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