General Methods for Handling Proteins

  • Robert A. Copeland


In this chapter we shall review some general methods for the day-to-day handling of proteins, and some of the precautions that must be taken to ensure proper activity and stability of these biopolymers. Some useful methods for altering the solution conditions for protein samples will also be discussed here.


Ammonium Sulfate Protein Solution Dialysis Tubing Molecular Weight Cutoff Handling Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bollag, D. M., and Edelstein, S. J. (1991) Protein Methods, Wiley, New York.Google Scholar
  2. Cooper, T. (1977) The Tools of Biochemistry, Wiley, New York.Google Scholar
  3. Craig, L. C., and King, T. P. (1957) J. Am. Chem. Soc., 79, 3729.CrossRefGoogle Scholar
  4. Deutscher, M. P. (1990) Guide to Protein Purification (Meth. Enzymol., 182), Academic Press, San Diego.Google Scholar
  5. Everse, J., and Stolzenbach, F. E. (1971) Meth. Enzymol., 22, 33–39.CrossRefGoogle Scholar
  6. Falson, P. (1992) Biotechniques, 13, 20–21.PubMedGoogle Scholar
  7. Fersht, A. (1985) Enzyme Structure and Function, 2d ed., W. H. Freeman, New York.Google Scholar
  8. Hjelmeland, L. M., and Chrambach, A. (1984) Meth. Enzymol., 104, 305–318.PubMedCrossRefGoogle Scholar
  9. Ingham, K. C. (1984) Meth. Enzymol., 104, 351–355.PubMedCrossRefGoogle Scholar
  10. Overall, C. M. (1987) Analyt. Biochem., 165, 208–214.PubMedCrossRefGoogle Scholar
  11. Pace, C. N. (1975) CRC Crit. Rev. Biochem., 3, 1–43.PubMedCrossRefGoogle Scholar
  12. Scopes, R. K. (1982) Protein Purification: Principles and Practice, Springer-Verlag, New York.Google Scholar
  13. Seed, B. (1989) in Short Protocols in Molecular Biology (Ausubel, F. M. et al., Ed.) Wiley, New York, p. 365.Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1994

Authors and Affiliations

  • Robert A. Copeland
    • 1
  1. 1.Experimental StationThe DuPont Merck Pharmaceutical CompanyWilmingtonUSA

Personalised recommendations