Effects of Chronic Ethanol Feeding on Rat Hepatocytes
Ethanol was fed at a level of 35% of total caloric intake to mature male rats for intervals of one to 38 days. Mitochondrial content of cytochrome c oxidase per mg protein was maximally depressed, to 40% of control values, after 10 days feeding. After 15 days of ethanol feeding, leucine incorporation into total hepatocyte protein, as well as into total intracellular (subcellular fractions) or.extracellular (secreted) proteins was substantially inhibited. At the level of total protein, alterations in isotope ratio as a consequence of ethanol feeding were only manifest in the secreted proteins. However, when component proteins of subcellular fractions were separated by gel electrophoresis, two specific early effects of ethanol feeding on protein synthesis by the endoplasmic reticulum could be detected. Aberrant isotope ratios established that synthesis of two entities, of 20000 and 50000 apparent molecular weight, was depressed after intervals of one and five days, respectively, feeding of 10% ethanol.
KeywordsSubcellular Fraction Ethanol Ingestion Chronic Ethanol Leucine Incorporation Ethanol Feeding
Unable to display preview. Download preview PDF.
- Khawaja, J.A. and Lindholm, D.B., 1978, Differential effect of ethanol ingestion on the protein synthetic activities of free and membrane-bound ribosomes from liver of the Weanling rat, Res. Comm. Chem. Pathol. Pharmacol., 19: 129.Google Scholar
- Rubin, E., Beattie, D.S., and Lieber, C., 1970, Effects of ethanol on the biogenesis of mitochondrial membranes and associated mitochondrial functions, Labor. Invest., 23: 620.Google Scholar
- Viskup, R.W., Baker, M., Holbrook, J.P., and Penniall, R., 1979, Age-associated changes in activities of rat hepatocytes, Experimental Aging Res., in press.Google Scholar