Isolation of a Lipocyte-Rich Fraction from Rat Liver Nonparenchymal Cells

  • David A. Otto
  • Richard L. Veech

Summary

A modified pronase digestion procedure is described for isolating nonparenchymal liver cells from vitamin A treated rats, which yielded a 15–23% population of lipocytes in the total cell suspension. The criteria for defining a lipocyte was the appearance of vitamin A containing cells as determined by fluorescent microscopy. Measurement of alcohol dehydrogenase and retinol dehydrogenase activities indicated that these enzyme activities were not present in the isolated nonparenchymal cells. A lipocyte-rich fraction of nonparenchymal cells was obtained by centrifugation of purified nonparenchymal cells in a linear Metrizamide gradient. Vitamin A fluorescence and chemical assay of vitamin A in the cell fractions indicated a four-fold enrichment of lipocytes in the cell fraction with d = 1.043 g/ml. Fractions high in vitamin A also had numerous cells with fat droplets as shown by transmission electron microscopy.

Keywords

Parenchymal Cell Alcohol Dehydrogenase Nonparenchymal Cell Nonparenchymal Liver Cell Retinol Acetate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1980

Authors and Affiliations

  • David A. Otto
    • 1
  • Richard L. Veech
    • 1
  1. 1.Laboratory of MetabolismNational Institute on Alcohol Abuse and AlcoholismRockvilleUSA

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