Immune Response in Strain 2 Guinea Pigs to the Syngeneic L2C Leukemia

  • Manuel J. RicardoJr.
  • Daniel T. Grimm
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 166)


The L2C leukemia is a transplantable acute B lymphoblastic leukemia that arose spontaneously in a female strain 2 guinea pig (1). The presence of immunoglobulin (Ig) and complement receptors on the surface of L2C tumor cells has established the B lymphocyte lineage of this leukemia (2). Five closely related mutant sublines of the L2C leukemia have been described (3) and are presumed to be derived from the same stem cell on the basis of cytogeneic analyses (4). The sublines share a common IgM idiotypic (id) determinant(s) and can be classified into two major groups, those that have detectable immune-associated antigens (Ia) on their cell surface and those that lack detectable Ia antigens on the basis of serological analysis (3). The presence of Ia antigens is apparently important in eliciting immunity against the tumor because effective L2C tumor protection is induced in normal strain 2 guinea pigs when immunized with Ia+ L2C tumor cells in adjuvant, but not generally with Ia- L2C tumor cells (3). However, immunization with Ia+ L2C cells protects the strain 2 guinea pigs against any of the 5 sublines. The latter finding supports the early observations made by Gross (5) that some strain 2 guinea pigs, when given a small dose of viable L2C tumor cells, developed resistance to a secondary challenge of L2C inoculum that produced fatal disease in syngeneic animals not previously given L2C tumorcells. Taken together, these results indicate that a tumor-associated transplantation antigen(s) (TATA) is present on L2C lymphoblasts. The IgM id determinants on the L2C cell surface have been implicated as a TATA for this tumor (6).


Normal Rabbit Serum Bowman Gray School Binding Competition Experiment States Public Health Service Grant Immune Splenocytes 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Congdon, C. C., and E. Lorenz. 1954. Leukemia in guinea pigs. Am. J. Pathol. 30: 337–359.PubMedGoogle Scholar
  2. 2.
    Shevach, E. M., L. Ellman, J. M. Davie, and I Green. 1972. L2C guinea pig lymphatic leukemia: a “B” cell leukemia. Blood 39: 1–12.PubMedGoogle Scholar
  3. 3.
    Forni, G., E. M. Shevach, and I. Green. 1976. Mutant lines of guinea pig L2C leukemia. I Deletion of Ia alloantigens is associated with a loss in immunogenicity of tumor-associated transplantation antigens. J. Exp. Med. 143: 1067–1081.PubMedCrossRefGoogle Scholar
  4. 4.
    Whang-Peng, J. 1977. Cytogenetic studies in L2C leukemia. Fed. Proc. 36: 2255–2259.PubMedGoogle Scholar
  5. 5.
    Gross, L. 1970. Specific, active intradermal immunization against leukemia in guinea pigs. Acta Haemat. 44: 1–10.PubMedCrossRefGoogle Scholar
  6. 6.
    Hu, C. P., B. O. Schwartz, and I. Green. 1978. Identification of a tumor-associated antigen of the guinea pig L2C leukemia by using syngeneic antisera. J. Immunol. 120: 579–601.Google Scholar
  7. 7.
    Ricardo, M. J., Jr., and D. T. Grimm- Preferential expression of IgGl antibodies specific for the L2C leukemia IgM idiotypic determinants in tumor-protected strain 2 guinea pigs. Immunol. (in press).Google Scholar
  8. 8.
    Ricardo, M. J., Jr., 1980. Heterogeneity of Fcreceptors on guinea pig T lymphocytes. J. Immunol. 123: 2009–2016.Google Scholar
  9. 9.
    Ricardo, M. J., Jr., 1981. Ísotype specificity of FcX-receptors on guinea pig T lymphocytes and their modulation by homologous immune complexes. Immunol. 42: 459–467.Google Scholar
  10. 10.
    Shevach, E. M., W. E. Paul, and I. Green. 1972. Histocompatibility-linked immune response gene function in the guinea pigs. J. Exp. Med. 136: 1207–1221.PubMedCrossRefGoogle Scholar
  11. 11.
    Schwartz, B. D., M. McMillan, E. Shevach, Y. Hohn, S. M. Rose, and L. Hood. 1980. Partial N-terminal amino acid sequences of guinea pig classic histocompatibility antigens. J. Immunol. 125: 1055–1059.PubMedGoogle Scholar
  12. 12.
    Stevenson, G. T., and F. K. Stevenson. 1975. Antibody to a molecularly-defined antigen to a tumor cell surface. Nature 254: 714–716.PubMedCrossRefGoogle Scholar
  13. 13.
    Bradley, B. A., and H. Festenstein. 1978. Cellular typing. Br. Med. Bull. 34: 223–232.PubMedGoogle Scholar
  14. 14.
    Bloch, K. J., F. M. Kourilsky, Z. Ovary and B. Benacerraf. 1963. Properties of guinea pig 7S antibodies. III Identification of antibodies involved in complement fixation and hemolysis. J. Exp. Med. 117: 965–981.PubMedCrossRefGoogle Scholar
  15. 15.
    Ohlander, C., A. Larson, and P. Perlmann. 1978. Specificity of Fc-receptors on lymphocytes and monocytes for guinea pig IgGl and IgG2: Induction and inhibition of cytolysis or phagocytosis of erythrocytes. Scand. J. Immunol. 7: 285–296.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • Manuel J. RicardoJr.
    • 1
  • Daniel T. Grimm
    • 2
  1. 1.Department of Microbiology and Immunology, Winston-SalemBowman Gray School of Medicine of Wake Forest UniversityUSA
  2. 2.Department of PediatricsThe University of Tennessee Center for the Health SciencesMemphisUSA

Personalised recommendations