Interferon Hybrids: Prospects for Therapy
Recombinant DNA techniques have led to determination of the structure of human interferons and production of individual molecular species of interferon free from other species and other proteins simultaneously induced in human cell cultures. Comparison of the properties of different molecular species of interferon and molecular hybrids between the various species has indicated the extent to which the various biological properties of interferons may be separated. This information provides a basis for considering the design of particular molecular constructions of interferons with particular desired properties. The production of hybrid interferons from different genes with common restriction enzyme sites provides a method for producing desirable analogs. The current data are here reviewed with respect to potential clinical utility of such materials.
KeywordsAntiviral Activity Antiviral Effect Human Interferon Human Cell Culture Individual Molecular Species
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- 3.Franke, A. E., H. M. Shepard, C. M. Houck, P. W. Leung, D. V. Goeddel, and R. M. Lawn. 1982. Carboxy terminal region of hybrid leukocyte interferons affects antiviral specificity. DNA (in press).Google Scholar
- 5.Wetzel, R., H. L. Levine, D. A. Estell, S. Shive, J. Finer-Moore, R. M. Stroud, and T. A. Bewley. 1982. Structure-function studies on human alpha interferon. In T. Merigan and R. Friedman (eds.), Chemistry and biology of interferons: Relationship to therapeutics. UCLA Symp. Mol. Cell. Biol. 25, Academic Press, New York (in press).Google Scholar
- 9.Harkins, R. N., P. E. Hass, W. H. Kohr, B. B. Aggarwal, P. K. Weck, and S. Apperson. 1982. Structural and biological properties of purified bacteria derived human fibroblast interferon. Proc. Natl. Acad. Sci. (in press).Google Scholar
- 11.Weck, P. K., S. Apperson, E. Hamilton, and N. Stebbing. 1982. Biological properties of genetic hybrids of bacteria-derived human leukocyte interferons. J. Cell. Biochem. Suppl. 6: 104.Google Scholar
- 17.Stebbing, N., P. K. Weck, J. T. Fenno, S. Apperson, and S. H. Lee. 1981. Comparison of the biological properties of natural and recombinant DNA derived human interferons, p. 25–33. In E. DeMaeyer, G. Galasso and H. Schellekens (eds.), The biology of the interferon system. Elsevier/North Holland.Google Scholar
- 21.Stebbing, N., P. K. Weck, J. T. Fenno, D. A. Estell, and E. Rinderknecht. 1982. Antiviral effects of bacteria derived human leukocyte interferons against encephalomyocarditis virus infection of squirrel monkeys. Arch. Virol. (in press).Google Scholar
- 23.Stebbing, N., S. H. Lee, B. J. Marafino, P. K. Weck, and K. W. Renton. 1982. Activity of cloned gene products in animal systems. In F. Ahmad, J. Schultz and E. E. Smith (eds.), From gene to protein: Translation into biotechnology. Academic Press, New York (in press).Google Scholar
- 30.Lee, S. H., P. K. Weck, J. Moore, S. Chen, and N. Stebbing. 1982. Pharmacological comparison of two hybrid recombinant DNA derived human leukocyte interferons. In T. Merigan and R. Friedman (eds.), Chemistry and biology of interferons: Relationship to therapeutics. UCLA Symp. Mol. Cell. Biol. 25, Academic Press, New York (in press).Google Scholar
- 31.Moore, J. A., B. J. Marafino, and N. Stebbing. 1982. Influence of various purified interferons on pharmacological effects of drugs in mice. J. Path. (in press).Google Scholar