Phospholipid Absorption and Diffusion through Membranes

  • Alessandro Bruni
  • Fabrizio Bellini
  • Lucia Mietto
  • Elena Boarato
  • Giampietro Viola

Abstract

Phospholipids are constituents of cell membranes. A main field of investigation is the study of mechanisms directing their synthesis and their selected distribution in different membranes of the cell. However, it is now widely appreciated that, together with a structural function, phospholipids have regulatory activity inside the cell, within the plasma membrane, as well as outside the cell (9). In their natural position, phospholipids modulate the activity of membrane-bound enzymes. Furthermore, the operation of selected synthetic pathways yields phospholipid derivatives behaving as second messengers. Thus, it is found that phosphatidic acid (PA), a phospholipid produced during the activation of signalling mechanisms, from the phosphorylation of diacyl glycerol (DAG) or by the action „f phospholipase D, may activate the superoxide production in neutrophils (2). Also, a lysophosphatidylserine analogue bearing an ether group at the sn-1 position of glycerol, acts as a negative modulator of the interaction between steroids and their intracellular receptors (5). Within the plasma membrane phospholipids serve as a substrate for the receptor-dependent phospholipases involved in the transduction of external stimuli into intracellular signals. Second messengers such as DAG are generated by these reactions. Outside the cells phospholipids behave as auto-pharmacological agents (autacoids, 6). For example, phosphatidylserine exposed to the extracellular environment activates the monocyte-macrophage system (30), exogenous PA is translocated inside the cell in the form of DAG (25), lysophosphatidylserine activates mouse mast cells (4) and phosphatidylserine (PS) vesicles interact with lymphocytes (20). The purpose of this review is to outline unifying aspects of these different actions of phospholipids.

Keywords

Mast Cell Nerve Growth Factor Phosphatidic Acid Phosphatidic Acid Mucosal Epithelial Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Allen, T. M., Williamson, P., and Schlegel, R. A., 1988, Phosphatidylserine as a determinant of reticuloendothelial recognition of liposome models of the erythrocyte surface, Proc. Natl. Acad, Sci. USA 85:8067–8071.CrossRefGoogle Scholar
  2. 2.
    Bellavite, P., Corso, F., Dusi, S., Grzeskowiak, M., Delia Bianca, V., and Rossi, F., 1988, Activation of NADPH-dependent Superoxide production in plasma membrane extracts of pig neutrophils by phosphatidic acid, J. Biol. Chem. 263:8210–8214.PubMedGoogle Scholar
  3. 3.
    Bellini, F., Toffano, G., Bruni, A., 1988, Activation of phosphoinositide hydrolysis by nerve growth factor and lysophosphatidylserine in rat peritoneal mast cells, Biochim. Biophys. Acta 970:187–193.PubMedCrossRefGoogle Scholar
  4. 4.
    Boarato, E., Mietto, L., Toffano, G., Bigon, E., and Bruni, A., 1984, Different responses of rodent mast cells to phosphatidylserine, Agents & Actions 14:613–618.CrossRefGoogle Scholar
  5. 5.
    Bodine, P. V., and Litwack, G., 1988, Purification and structural analysis of the modulator of the glucocorticoid-receptor complex. Evidence that the modulator is a novel phosphoglyceride, J. Biol. Chem. 263:3501–3512.PubMedGoogle Scholar
  6. 6.
    Bruni, A., 1988, Autacoids from membrane phospholipids, Pharmacol. Res. Commun. 20:529–544.PubMedCrossRefGoogle Scholar
  7. 7.
    Bruni, A., Bigon, E., Battistella, A., Boarato, E., Mietto, L., and Toffano, G., 1984, Lysophosphatidylserine as histamine releaser in mice and rats, Agents & Actions 14:619–625.CrossRefGoogle Scholar
  8. 8.
    Bruni, A., Bigon, E., Boarato, E., Mietto, L., Leon, A., and Toffano, G., 1982, Interaction between nerve growth factor and lysophosphatidylserine on rat peritoneal mast cells, FEBS Letters 138:190–192.PubMedCrossRefGoogle Scholar
  9. 9.
    Bruni, A., Mietto, L., Battistella, A., Boarato, E., Palatini P., and Toffano, G., 1986, Serine phospholipids in cell communication, in: “Phospholipids Research and the Nervous System,” L. Horrocks, L. Freysz and G. Toffano, eds., Fidia Research Series, Vol. 14, Liviana Press, Padova, pp. 217–223.Google Scholar
  10. 10.
    Chang, H. W., Inoue, K., Bruni, A., Boarato, E., Toffano, G., 1988, Stereoselective effects of lysophosphatidylserine in rodents, Br. J. Pharmac. 93:647–653.CrossRefGoogle Scholar
  11. 11.
    Daleke, D. L., and Huestis W. H., 1985, Incorporation and translocation of aminophospholipids in human erythrocytes, Biochemistry 24:5406–5416.PubMedCrossRefGoogle Scholar
  12. 12.
    Esko, J. D., and Matsuoka, K. Y., 1983, Biosynthesis of phosphatidylcholine from serum phospholipids in Chinese hamsters ovary cells deprived of choline, J. Biol. Chem. 258:3051–3057.PubMedGoogle Scholar
  13. 13.
    Esko, J. D., Nishijima, M., and Raetz, C. R. H., 1982, Animal cells dependent on exogenous phosphatidylcholine for membrane biogenesis, Proc. Natl. Acad. Sci. USA 79:1698–1702.PubMedCrossRefGoogle Scholar
  14. 14.
    Gilbreath, M. J., Hoover, D. L., Alving, C. R., Swartz, G. M., Meltzer, M. S., 1986, Inhibition of lymphokine-induced macrophage microbicidal activity against Leishmania major by liposomes: characterization of the physicochemical requirements for liposome inhibition, J. Immunol. 137:1681–1687.PubMedGoogle Scholar
  15. 15.
    Gilbreath, M. J., Nacy, C.A., Hoover, D. L., Alving, C. R., Swartz, G. M., and Meltzer, M. S., 1985, Macrophage activation for microbicidal activity against Leishmania major: inhibition of lymphokine activation by phosphatidylcholine-phosphatidylserine liposomes, J, Immunol. 134:3420–3425.Google Scholar
  16. 16.
    Irvine, R. F., and Dawson, R. M. C., 1979, Transfer of arachidonic acid between phospholipids in rat liver microsomes, Biochem. Biophys. Res. Commun. 91:1399–1405.PubMedCrossRefGoogle Scholar
  17. 17.
    Mansbach, C. M. II, 1977, The origin of chylomicron phosphatidylcholine in the rat, J. Clin. Invest. 60:411–420.PubMedCrossRefGoogle Scholar
  18. 18.
    Mansbach, C. M. II, and Parthasarathy, S., 1979, Regulation of de novo phosphatidylcholine synthesis in rat intestine, J. Biol. Chem. 254:9688–9694.PubMedGoogle Scholar
  19. 19.
    Mietto, L., Boarato, E., Toffano, G., and Bruni, A., 1987, Lysophosphatidylserine-dependent interaction between rat leukocytes and mast cells, Biochim. Biophys. Acta 930:145–153.PubMedCrossRefGoogle Scholar
  20. 20.
    Mietto, L., Boarato, E., Toffano, G., and Bruni, A., 1989, Internalization of phosphatidylserine by adherent and non-adherent rat mononuclear cells. Biochim. Biophys. Acta 1013:1–6.PubMedCrossRefGoogle Scholar
  21. 21.
    Mohandas, N., Wyatt, J., Mel, S. F., Rossi, M. E., and Shohet, S. B., 1982, Lipid translocation across the human erythrocyte membrane. Regulatory factors, J. Biol. Chem. 257:6537–6543.PubMedGoogle Scholar
  22. 22.
    Moll, G. N., Vial, H. J., Ancelin, M. L., Op den Kamp, J. A. F., Roelofsen, B., and van Deenen, L. L. M., 1988, Phospholipid uptake by Plasmodium knowlesi infected erythrocytes, FEBS Lett. 232:341–346.PubMedCrossRefGoogle Scholar
  23. 23.
    Moolenaar, W. H., Kruijer, W., Tilly, B. C., Verlaan, I., Bierman, A. J., and de Laat, S.W., 1986, Growth factor-like action of phosphatidic acid, Nature 323:171–173.PubMedCrossRefGoogle Scholar
  24. 24.
    Nishijima, M., Kuge, O., and Akamatsu, Y., 1986, Phosphatidylserine biosynthesis in cultured Chinese hamster ovary cells I. Inhibition of de novo phosphatidylserine biosynthesys by exogenous phosphatidylserine and its efficient incorporation, J. Biol. Chem. 261:5784–5789.PubMedGoogle Scholar
  25. 25.
    Pagano, R. E., and Sleight, R. G., 1985, Defining lipid transport pathways in animal cells, Science 229:1051–1057.PubMedCrossRefGoogle Scholar
  26. 26.
    Schroit, A. J., Tanaka, Y., Madsen, J., and Fidler, I. J., 1984, The recognition of red blood cells by macrophages: role of phosphatidylserine and possible implications of membrane phospholipid asymmetry, Biol. Cell 51:227–238.PubMedCrossRefGoogle Scholar
  27. 27.
    Seigneuret, M., and Devaux, P.F., 1984, ATP-dependent asymmetric distribution of spin-labeled phospholipids in the erythrocyte membrane: relation to shape changes, Proc. Natl. Acad. Sci. USA 81:3751–3755.PubMedCrossRefGoogle Scholar
  28. 28.
    Senior, J., and Gregoriadis, G., 1982, Stability of small unilamellar liposomes in serum and clearance from the circulation: the effect of the phospholipid and cholesterol components, Life Sci. 30:2123–2136.PubMedCrossRefGoogle Scholar
  29. 29.
    Suzuki, T., Saito-Taki, T., Sadasivan, R., and Nitta, T., 1982, Biochemical signal transmitted by Fcγ receptors: phospholipase A2 activity of Fcγ 2b receptor of murine macrophage cell line P388D1, Proc. Natl. Acad. Sci. USA 79:591–595.PubMedCrossRefGoogle Scholar
  30. 30.
    Tanaka, Y., and Schroit, A. J., 1983, Insertion of fluorescent phosphatidylserine into the plasma membrane of red blood cells. Recognition by autologous macrophages, J. Biol. Chem. 258:11335–11343.PubMedGoogle Scholar
  31. 31.
    Vance, J. E., 1988, Compartmentalization of phospholipids for lipoprotein assembly on the basis of molecular species and biosynthetic origin, Biochim. Biophys. Acta 963:70–81.PubMedCrossRefGoogle Scholar
  32. 32.
    Wassef, N. M., Roerdink, F., Richardson, E. C., and Alving, C. R., 1984, Suppression of phagocytic function and phospholipid metabolism in macrophages by phosphatidylinositol liposomes, Proc. Natl. Acad. Sci. USA 81:2655–2659.PubMedCrossRefGoogle Scholar
  33. 33.
    Yui, S., and Yamazaki, M., 1987, Relationship of ability of phospholipids to stimulate growth and bind to macrophages, J. Leukocyte Biol. 41:392–399.PubMedGoogle Scholar
  34. 34.
    Zachowski, A., Favre, E., Cribier, S., Hervé, P., and Devaux, P. F., 1986, Outside-inside translocation of aminophospholipids in the human erythrocyte membrane is mediated by a specific enzyme, Biochemistry 25:2585–2590.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Alessandro Bruni
    • 1
    • 2
  • Fabrizio Bellini
    • 2
  • Lucia Mietto
    • 2
  • Elena Boarato
    • 2
  • Giampietro Viola
    • 2
  1. 1.Department of PharmacologyUniversity of PadovaPadovaItaly
  2. 2.Fidia Research LaboratoriesAbano TermeItaly

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