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Immunologic Analysis of the Acetylcholine Receptor

  • Chapter
Ion Channel Reconstitution

Abstract

Studies of receptors have come a long way in the past decade or so. It is no longer possible to study their pharmacological or electrophysiological properties without serious consideration of their macromolecular properties. A cascade of new techniques (e.g., affinity labeling, affinity purification, reconstitution, patch clamping, monoclonal antibody production, gene cloning, in vitro gene expression, antisense mRNA) is overcoming many of the old barriers to molecular characterization of receptors (e.g., small amounts of receptor, lack of biochemical probes, lack of techniques for solubilizing, reconstituting, and characterizing receptors in membranes). In this cascade of techniques, today’s new technique (e.g., in vitro mutagenesis) frequently seems to threaten to make yesterday’s new technique (e.g., reconstitution) seem passe even before the approach can be thoroughly established and utilized. In fact, all of these techniques have virtues and limitations, and many of these techniques will be required in state-of-the-art studies of receptors. It is clear, however, that a continuance of productivity in studies of receptors will require knowledge of several of these techniques. One of the technologies that has been and will continue to be useful in studies of receptors from the initial stages of identification through purification and characterization and into studies of synthesis and the molecular basis of function is the use of antibodies.

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Authors and Affiliations

  1. The Salk Institute, San Diego, California, 92138, USA

    Jon Lindstrom

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  1. Jon Lindstrom
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  1. Brandeis University, Waltham, Massachusetts, USA

    Christopher Miller

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© 1986 Springer Science+Business Media New York

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Lindstrom, J. (1986). Immunologic Analysis of the Acetylcholine Receptor. In: Miller, C. (eds) Ion Channel Reconstitution. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-1361-9_9

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  • DOI: https://doi.org/10.1007/978-1-4757-1361-9_9

  • Publisher Name: Springer, Boston, MA

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