Incorporation of Ion Channels by Fusion
From the time when Mueller and colleagues (1962) first developed the planar lipid bilayer, a major goal has been to incorporate biological membrane transport proteins into these electrically accessible model membranes. One important group of transporters is that of the ion-channel proteins. Many different ion channels have been identified in biological membranes, and it is important to investigate them in single-channel experiments. Just as patch recording (Hamill et al., 1981) is the only method for observing single channels in their native membranes, the planar bilayer has thus far proven to be the system of choice for studying single channels in model membranes of defined composition. The insertion of channel proteins into planar bilayers is thus the major problem that must be solved before such reconstitution experiments can be undertaken.
KeywordsFusion Process Fusion Event Planar Lipid Bilayer Planar Bilayer Patch Recording
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