Analysis and Chemical Modification of Bacterial Porins

  • Roland Benz
Chapter

Abstract

The cell envelope of gram-negative bacteria such as Escherichia coli, Salmonella typhimurium, and Pseudomonas aeruginosa consists of three different layers, the outer membrane, the peptidoglycan (murein) layer, and the inner membrane (Nikaido, 1979a; Beveridge, 1981). The inner membrane acts as a real diffusion barrier and contains, in addition to the respiration chain and the H+ -ATPase, a large number of different transport systems for substrates. The peptidoglycan or murein layer consists of repeating N-acetylglucosaminyl-N-acetylmuramyl dimers, which form a macromolecule surrounding the cell (Beveridge, 1981). This layer protects the cells from lysis. The outer membrane is a molecular filter with defined exclusion limits for hydrophilic substrates (Nikaido and Nakae, 1979). In enteric bacteria, the outer membrane acts also as a barrier for hydrophobic compounds such as detergents (Nikaido, 1979b). The active substrates of the molecular sieving properties for hydrophilic substances are a major class of proteins called matrix proteins (Rosenbusch, 1974) or porins (Nakae, 1976).

Keywords

Outer Membrane Outer Membrane Protein Lipid Bilayer Membrane Selectivity Filter General Diffusion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer Science+Business Media New York 1986

Authors and Affiliations

  • Roland Benz
    • 1
  1. 1.Department of BiologyUniversity of KonstanzKonstanzWest Germany

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